表面活性剂辅助超声萃取杜仲叶总多酚及其抗氧化活性研究

doi:10.3969/j.issn.1001-1803.2020.03.005

日用化学工业 ›› 2020, Vol. 50 ›› Issue (3): 164-170.doi: 10.3969/j.issn.1001-1803.2020.03.005

表面活性剂辅助超声萃取杜仲叶总多酚及其抗氧化活性研究

李陆军^1,²,王志宏¹,霍延平²,王翔¹,杨秋玲¹,彭密军¹()

^1. 广东省测试分析研究所广东省分析测试技术公共实验室,广东广州 510070
^2. 广东工业大学轻工化工学院,广东广州 510006

收稿日期:2019-08-02 修回日期:2020-03-06 出版日期:2020-03-22 发布日期:2020-04-02
通讯作者: 彭密军
作者简介:李陆军（1993-）,男,河南人,硕士研究生,电话：13113962986,E-mail： 570522940@qq.com。
基金资助:
广东省科学院发展专项资金项目资助(2019GDASYL-0502003);国家自然科学基金资助项目(31660181);杜仲综合利用技术国家地方联合工程实验室项目资助(NLE201602);杜仲综合利用技术国家地方联合工程实验室项目资助(NLE201704)

Study on surfactant-assisted ultrasonic extraction of total polyphenols from Eucommia ulmoides leaves and their antioxidant activity

Lu-jun LI^1,²,Zhi-hong WANG¹,Yan-ping HUO²,Xiang WANG¹,Qiu-ling YANG¹,Mi-jun PENG¹()

^1. Guangdong Provincial Public Laboratory of Analysis and Testing Technology, Guangdong Institute of Analysis, Guangzhou, Guangdong 510070, China
^2. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, Guangdong 510006, China

Received:2019-08-02 Revised:2020-03-06 Online:2020-03-22 Published:2020-04-02
Contact: Mi-jun PENG

摘要/Abstract

摘要：

优化了表面活性剂辅助超声提取杜仲叶总多酚工艺,并比较了不同提取工艺的杜仲叶提取物的体外抗氧化能力。首先以总多酚为指标,在单因素试验的基础上,通过正交试验优化杜仲叶总多酚的提取条件。然后以提取物的羟基自由基清除率、ABTS自由基清除率、DPPH自由基清除率和还原力来评价杜仲叶提取物的体外抗氧化能力。实验结果表明较佳提取工艺为SDS用量0.1 g/L,超声时间5 min,乙醇体积分数50%,料液比1∶20 （g∶mL）,温度45 ℃,在该条件下,杜仲叶总多酚得率为6.770%。杜仲叶提取物具有一定的体外抗氧化能力。

关键词: 杜仲叶, 总多酚, 提取工艺, 表面活性剂, 抗氧化

Abstract:

The surfactant-assisted ultrasonic extraction of total polyphenols from Eucommia ulmoides leaves was optimized. The antioxidant activity in vitro was compared between the extracts from Eucommia ulmoides leaves with different extraction processes. Firstly, on the basis of the single-factor test, the total polyphenols were used as indicators to optimize the extraction conditions by the orthogonal experiment. Then the antioxidant activity of the extract from Eucommia ulmoides leaves was evaluated in vitro in terms of the hydroxyl radical scavenging rate, ABTS free radical scavenging rate, DPPH free radical scavenging rate and reducing power. The experimental results showed that the optimal extraction process was SDS dosage of 0.1 g/L, ultrasonic extraction time of 5 min, ethanol volume fraction of 50%, solid-liquid ratio of 1∶20 （g∶mL）, and temperature of 45 °C. Under this condition, the total polyphenol yield of Eucommia ulmoides leaves was 6.770%. The extract from Eucommia ulmoides leaves had certain antioxidant activity in vitro.

Key words: Eucommia ulmoides leaf, total polyphenols, extraction process, surfactant, antioxidation

中图分类号:

TQ658

李陆军,王志宏,霍延平,王翔,杨秋玲,彭密军. 表面活性剂辅助超声萃取杜仲叶总多酚及其抗氧化活性研究[J]. 日用化学工业, 2020, 50(3): 164-170.

Lu-jun LI,Zhi-hong WANG,Yan-ping HUO,Xiang WANG,Qiu-ling YANG,Mi-jun PENG. Study on surfactant-assisted ultrasonic extraction of total polyphenols from Eucommia ulmoides leaves and their antioxidant activity[J]. China Surfactant Detergent & Cosmetics, 2020, 50(3): 164-170.

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参考文献 12

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水平	A 乙醇体积分数/%	B 料液比/（g·mL^-1）	C 温度/℃
1	30	1∶15	35
2	50	1∶20	40
3	70	1∶25	45

表面活性剂	类型	杜仲总多酚得率/%
吐温20	非离子型表面活性剂	6.224
聚乙二醇	非离子型表面活性剂	6.219
十二烷基硫酸钠	阴离子型表面活性剂	6.463
十二烷基磺酸钠	阴离子型表面活性剂	6.445
十六烷基三甲基溴化铵	阳离子型表面活性剂	6.342
空白		6.217

因素	A	B	C		得率/%
实验1	1	1	1	1	5.220
实验2	1	2	2	2	5.430
实验3	1	3	3	3	5.560
实验4	2	1	2	3	6.100
实验5	2	2	3	1	6.770
实验6	2	3	1	2	6.450
实验7	3	1	3	2	6.600
实验8	3	2	1	3	6.360
实验9	3	3	2	1	6.580
均值1	5.403	5.973	6.010	6.190
均值2	6.440	6.187	6.037	6.160
均值3	6.513	6.197	6.310	6.007
极差水平	1.110 A₃	0.224 B₃	0.300 C₃	0.183 D₁

因素	偏差平方和	自由度	F比	F临界值	显著性
乙醇体积分数	2.312	2	39.86	19.00	*
料液比	0.095	2	1.638	19.00
温度	0.165	2	2.845	19.00

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表面活性剂辅助超声萃取杜仲叶总多酚及其抗氧化活性研究

Study on surfactant-assisted ultrasonic extraction of total polyphenols from Eucommia ulmoides leaves and their antioxidant activity

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