钙离子与碱性蛋白酶的相互作用及机理研究

doi:10.13218/j.cnki.csdc.2018.01.001

摘要/Abstract

摘要： 研究了Ca²⁺与碱性蛋白酶的相互作用,并通过动态光散射(DLS)实验和荧光光谱法探讨其作用机理。实验结果表明,Ca²⁺对碱性蛋白酶的活性具有一定的激活作用,其中CaCl₂较Ca(HCO₂)₂对碱性蛋白酶酶活的激活作用更加显著。动态光散射实验表明,Ca²⁺与碱性蛋白酶主要通过静电作用力结合,Ca²⁺的加入使得碱性蛋白酶分子的平均粒径减小,疏水结构逐渐向酶分子内部转移,有利于碱性蛋白酶活性的提高。利用荧光光谱研究Ca²⁺与碱性蛋白酶的结合作用,结果表明Ca²⁺对碱性蛋白酶的猝灭机制属于静态猝灭,Ca²⁺与碱性蛋白酶形成稳定的复合物,且结合能力很强,结合过程能够自发进行。

关键词: 碱性蛋白酶, 钙离子, 活性, 动态光散射, 荧光光谱

Abstract: The interaction of calcium ions and alkaline protease was studied.The mechanism of action was investigated by dynamic light scattering (DLS) and fluorescence spectroscopy.Results show that calcium ions have a certain activation effect on the activity of alkaline protease,in which calcium chloride activates the activity of alkaline protease more significantly than calcium formate.DLS experiments show that calcium ion and alkaline protease are mainly combined by electrostatic force.With the addition of calcium ions,the average particle size of the alkaline protease molecules decreased,and the hydrophobic structure gradually shift to the interior of the enzyme molecule,which is beneficial to the improvement of alkaline protease activity.The binding of calcium ions to alkaline protease was studied by fluorescence spectroscopy.Results show that the quenching mechanism of calcium ions to alkaline protease is a static quenching.Calcium ions form a stable complex with alkaline protease.The binding ability is strong,and the process can be spontaneous.

Key words: alkaline protease, calcium ion, activity, dynamic light scattering, fluorescence spectroscopy

中图分类号:

O629.8

李静, 张剑, 赵永祥. 钙离子与碱性蛋白酶的相互作用及机理研究[J]. 日用化学工业, 2018, 48(1): 1-7.

LI Jing, ZHANG Jian, ZHAO Yong-xiang. Interaction between calcium ions and alkaline protease and study of its mechanism[J]. China Surfactant Detergent & Cosmetics, 2018, 48(1): 1-7.

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