京尼平交联的丝素蛋白-壳聚糖微球的制备及表征

doi:10.13218/j.cnki.csdc.2014.06.073

日用化学工业 ›› 2014, Vol. 44 ›› Issue (6): 328-332.doi: 10.13218/j.cnki.csdc.2014.06.073

京尼平交联的丝素蛋白-壳聚糖微球的制备及表征

叶漫文¹, 曾曙光¹, 高文峰¹, 容明灯¹, 郭泽鸿¹, 石勇¹, 干辉勇²

1.南方医科大学附属口腔医院广东省口腔医院,广东广州 510220;
2.东莞市三局医院,广东东莞 523710

收稿日期:2014-05-04 出版日期:2014-06-22 发布日期:2019-04-10
通讯作者: 曾曙光,教授,主任医师,硕士生导师,E-mail:sunrisez@tom.com。
作者简介:叶漫文(1988-),男,江西人,硕士研究生,E-mail:spiritsealove@126.com。
基金资助:
广东省科技计划社会发展项目(2011B031300022);广东省卫生厅基金资助项目(B2012031)

Preparation and characterization of genipin-crosslinked silk fibroin/chitosan microspheres

YE Man-wen¹, ZENG Shu-guang¹, GAO Wen-feng¹, RONG Ming-deng¹, GUO Ze-hong¹, SHI Yong¹, GAN Hui-yong²

1.The Affiliated Stomatological Hospital of Southern Medical University & Guangdong Provincial Stomatological Hospital, Guangzhou,Guangdong 510220,China;
2.San Ju Hospital,Dongguan,Guangdong 523710,China

Received:2014-05-04 Online:2014-06-22 Published:2019-04-10

摘要/Abstract

摘要： 以乳化交联法制备京尼平交联的丝素蛋白(SF)-壳聚糖(CS)微球,考察了SF,CS和京尼平的用量对微球的表观形态、粒径、溶胀率和物理化学性质的影响。结果表明:以京尼平1.0 g,CS和SF各0.2 g;京尼平0.05 g,CS和SF各0.2 g;京尼平0.5 g,CS 0.2 g,SF 0.4 g;京尼平0.5 g,CS 0.2 g,SF 0.1 g;京尼平0.1 g,CS 0.2 g,SF 0.1 g等5种组合制备出的微球,形态较为规则,表面较光滑,粒径分布均匀,平均粒径分别为71.13,146.60,74.09,89.00和109.83 μm。经FTIR和XRD证实,京尼平使得SF和CS的氨基发生交联,SF的结构从α螺旋状或者无序蜷曲状改变为β折叠状。

关键词: 丝素蛋白, 壳聚糖, 微球, 制备, 交联

Abstract: Genipin-crosslinked silk fibroin (SF)/chitosan (CS) microspheres was prepared by emulsion crosslinking technique.Effect of dosage of CS,SF and genipin on surface morphology,size distribution,swelling ratio and physicochemical property of the microspheres were examined.Results showed that the microspheres which prepared by 1.0 g genipin,CS 0.2 g and SF 0.2 g;0.05 g genipin,CS 0.2 g and SF 0.2 g;0.5 g genipin,CS 0.2 g and SF 0.4 g;0.5 g genipin,CS 0.2 g and SF 0.1 g;0.1 g genipin,CS 0.2 g and SF 0.1 g,are rather regularly spherical and well distributed in shape,and their average diameters are about 71.13,146.60,74.09,89.00 and 109.83 μm,respectively.Characterization from FTIR and XRD results confirmed that genipin causes the crosslinking reaction of the amino groups of SF and CS.Further,genipin could induce structure of the SF from random coil shape or α-helix shape to β-folded sheet shape.

Key words: silk fibroin, chitosan, microspheres, preparation, crosslink

中图分类号:

TQ658

叶漫文, 曾曙光, 高文峰, 容明灯, 郭泽鸿, 石勇, 干辉勇. 京尼平交联的丝素蛋白-壳聚糖微球的制备及表征[J]. 日用化学工业, 2014, 44(6): 328-332.

YE Man-wen, ZENG Shu-guang, GAO Wen-feng, RONG Ming-deng, GUO Ze-hong, SHI Yong, GAN Hui-yong. Preparation and characterization of genipin-crosslinked silk fibroin/chitosan microspheres[J]. China Surfactant Detergent & Cosmetics, 2014, 44(6): 328-332.

参考文献

[1] CHIELLINI F,PIRAS A M,ERRICO C,et al.Micro/nanostructured polymeric systems for biomedical and pharmaceutical applications [J].Nanomed,2008,3(3):367-393.
[2] LIU J,LIU C,LIU Y,et al.Study on the grafting of chitosan-gelatin microcapsules onto cotton fabrics and its antibacterial effect [J].Colloids Surf B Biointerfaces,2013,109(1):103-108.
[3] ELEANOR M P,DAVID L K.Silk fibroin biomaterials for controlled release drug delivery [J].Expert Opin.Drug Deliv,2011,8(6):797-811.
[4] WEN X,PENG X,FU H,et al.Preparation and in vitro evaluation of silk fibroin microspheres produced by a novel ultra-fine particle processing system [J].International Journal of Pharmaceutics,2011,416(1):195-201.
[5] ZHANG J,TANG Q,XU X,et al.Development and evaluation of a novel phytosome-loaded chitosan microsphere system for curcumin delivery [J].International Journal of Pharmaceutics,2013,448(1):168-174.
[6] 叶漫文,曾曙光.基于丝素蛋白和壳聚糖的骨组织工程材料研究进展[J].广东牙病防治,2013,21(2):108-112.
[7] KAWADKAR J,CHAUHAN M K.Intra-articular delivery of genipin cross-linked chitosan microspheres of flurbiprofen:Preparation,characterization,in vitro and in vivo studies [J].Eur J Pharm Biopharm,2012,8(13):563-572.
[8] LIANG H C,CHANG W H,LIN K J,et al.Genipin-crosslinked gelatin microspheres as a drug carrier for intramuscular administration:in vitro and in vivo studies [J].J Biomed Mater Res,2003,65(2):271-282.
[9] ZHANG K,QIAN Y,WANG H,et al.Genipin-crosslinked silk fibroin/hydroxybutyl chitosan nanofibrous scaffolds for tissue-engineering application [J].J Biomed Mater Res A,2010,95(3):870-881.
[10] 张光宇,迟美玉,倪静,等.星点设计-效应面法优化去甲斑蝥素壳聚糖-丝素蛋白栓塞微球的制备[J].中国新药杂志,2011,20(17):1624-1630.
[11] CHIU Y T,CHIU C P,CHIEN J T,et al.Encapsulation of lycopene extract from tomato pulp waste with gelatin and poly(gamma-glutamic acid) as carrier [J].J Agric Food Chem,2007,55(13):5123-5130.
[12] LAMPRECHT A,SCHAÈFER U,LEHR C M,et al.Influences of process parameters on preparation of microparticle used as a carrier system for x3 unsaturated fatty acid ethyl esters used in supplementary nutrition [J].Journal of Microencapsulation,2001,18(3):347-357.
[13] NAKAGAWA K,IWAMOTO S,NAKAJIMA M,et al.Microchannel emulsification using gelatin and surfactant-free coacervate microencapsulation [J].Journal of Colloid and Interface Science,2004,278(1):198-205.
[14] SILVA D F,FÁVARO-TRINDADE C S,ROCHA G A,et al,Microencapsulation of lycopene by gelatin-pectin complex coacervation [J].Journal of Food Processing and Preservation,2012,36(2):185-190.
[15] ROCHA-SELMI G A,BOZZA F T,THOMAZINI M A,et al.Microencapsulation of aspartame by double emulsion followed by complex coacervation to provide protection and prolong sweetness [J].Food Chem,2013,15;139:72-78.
[16] 刘纯,金梦瑶,张学农.丝素蛋白-壳聚糖双氯芬酸钠缓释微球的制备与特性研究[J].中国新药杂志,2009,18(16):1566-1571.
[17] WANG X,YUCEL T,LU Q,et al.Silk nanospheres and microspheres from silk/pva blend films for drug delivery [J].Biomaterials,2010,31(6):1025-1035.

京尼平交联的丝素蛋白-壳聚糖微球的制备及表征

Preparation and characterization of genipin-crosslinked silk fibroin/chitosan microspheres

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