欢迎访问《日用化学工业（中英文）》，今天是
日用化学工业（中英文）

日用化学工业 ›› 2022, Vol. 52 ›› Issue (1): 20-27.doi: 10.3969/j.issn.1001-1803.2022.01.004

• 开发与应用 • 上一篇    下一篇

黄杜中药漱口液对牙周病相关菌的影响及作用机理研究

王晓宇1,王亚静1,*(),赵鑫2,*(),王雁雯1,王宇1,黄赞扬1   

  1. 1.天津中医药大学 现代中药发现与制剂技术教育部工程研究中心,天津 301617
    2.天津中医药大学 方剂学教育部重点实验室,天津 301617
  • 收稿日期:2021-04-06 修回日期:2021-12-06 出版日期:2022-01-22 发布日期:2022-01-19
  • 通讯作者: 王亚静,赵鑫
  • 基金资助:
    国家自然科学基金资助项目(81803959);天津市科技计划项目(19ZYPTJC00060);天津市科技计划项目(18ZXXYSY00130)

Study on the effect and mechanism of Huangdu Chinese herbal mouthwash on periodontal disease-related bacteria

Wang Xiaoyu1,Wang Yajing1,*(),Zhao Xin2,*(),Wang Yanwen1,Wang Yu1,Huang Zanyang1   

  1. 1. Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
    2. Key Research Laboratory of Prescription Compatibility among Components, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
  • Received:2021-04-06 Revised:2021-12-06 Online:2022-01-22 Published:2022-01-19
  • Contact: Yajing Wang,Xin Zhao

RichHTML

9

PDF (PC)

236

赞

摘要：

探究了黄杜中药漱口液对牙周病相关菌活性的影响及作用机理。分别以牙周病相关致病菌牙龈卟啉单胞菌（Porphyromonas gingivalis,P. gingivalis）和益生菌食窦魏斯氏菌（Weissella cibaria,W. cibaria）为受试菌,采用微量肉汤稀释法考察黄杜中药漱口液对P. gingivalis、W. cibaria活性的影响,结果显示其对致病菌P. gingivalis具有显著的抑制作用（稳定期生长率<20%）,对益生菌W. cibaria基本无抑制（稳定期生长率>80%）。通过考察细胞壁、细胞膜的完整性及能量代谢酶的活性进一步探究黄杜中药漱口液对受试菌的作用机理,结果显示,P. gingivalis胞外碱性磷酸酶（AKP）、K+、核酸、蛋白质含量显著升高（P<0.01）,能量代谢酶琥珀酸脱氢酶（SDH）、苹果酸脱氢酶（MDH）活性显著下降（P<0.01）,而对W. cibaria无显著影响（P>0.05）。推断出黄杜中药漱口液通过破坏致病菌P. gingivalis细胞膜、细胞壁完整性,扰乱能量代谢系统发挥抑菌作用,而对益生菌W. cibaria基本无影响,提示其可提高口腔益生菌的相对占比,改善口腔菌群,对牙周病的防治发挥积极作用。

关键词: 中药漱口液, 牙周病, 抑菌机理, 牙龈卟啉单胞菌, 食窦魏斯氏菌, 益生菌

Abstract:

The effect and mechanism of Huangdu Chinese herbal mouthwash on the activity of periodontal disease-related bacteria were explored. Periodontal disease-related pathogen Porphyromonas gingivalis and probiotic Weissella cibaria were selected as the indicator bacteria. The effect of Huangdu Chinese herbal mouthwash on the activity of P. gingivalis and W. cibaria was investigated by microbroth dilution method. From the perspective of the cell structure, the regulatory mechanism of Huangdu mouthwash on these two indicators was further investigated by detecting the integrity of cell wall and cell membrane and the activity of energy-metabolism enzymes. The results show that Huangdu mouthwash has significant inhibitory effect on pathogenic bacteria P. gingivalis （growth rate in stable period <20%）, and almost no inhibition on probiotic W. cibaria （growth rate in stable period >80%）. While cetylpyridinium chloride mouthwash significantly inhibits the growth of pathogen P. gingivalis and probiotic W. cibaria, which is almost in the lag phase （growth rate <10%） within 24 hours. Compared with the control group, the content of extracellular AKP, K+, nucleic acid and protein of pathogenic bacteria P. gingivalis is significantly increased （P<0.01）, while the activities of energy-metabolism enzymes SDH and MDH are significantly decreased by 19.84% and 10.31% （P<0.01）, but no significant effect on W. cibaria （P>0.05） is observed in the case of Huangdu mouthwash. It can be concluded that Huangdu mouthwash has bacteriostatic effect by destroying the integrity of cell membrane and cell wall of P. gingivalis and disturbing the energy-metabolism system, but it has little effect on probiotic W. cibaria. However, cetylpyridinium chloride mouthwash has both reduce the activities of pathogen and probiotic by destroying cell membrane and cell wall. The present work suggests that the Huangdu Chinese herbal mouthwash can increase the relative proportion of oral probiotics, improve oral flora, and thus provide a feasible reference for the prevention and treatment of periodontal disease.

Key words: Chinese herbal mouthwash, periodontal disease, antibacterial mechanism, Porphyromonas gingivalis, Weissella cibaria, probiotic

中图分类号: 

  • TQ658