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日用化学工业（中英文）

日用化学工业 ›› 2017, Vol. 47 ›› Issue (6): 326-330.doi: 10.13218/j.cnki.csdc.2017.06.006

• 开发与应用 • 上一篇    下一篇

透骨草抑菌活性成分提取及抑菌机理研究

张美龄1, 梁蓉1,2, 曹光群1, 杨成1   

  1. 1.江南大学 化学与材料工程学院,江苏 无锡 214122;
    2.江南大学 食品胶体与生物技术教育部重点实验室,江苏 无锡 214122
  • 收稿日期:2016-12-29 发布日期:2019-03-20
  • 通讯作者: 曹光群,高工,电话:13706182565,E-mail:gqcao59@126.com。
  • 作者简介:张美龄(1992-),男,江西人,硕士研究生,电话:18352502536,E-mail:meiling_zhang@foxmail.com。
  • 基金资助:
    国家自然科学基金青年基金资助项目(31401533)

Extraction for antibacterial active ingredient of Phryma leptostachya L.and its mechanism for bacteria inhibition

ZHANG Mei-ling1, LIANG Rong1,2, CAO Guang-qun1, YANG Cheng1   

  1. 1.School of Chemical and Material Engineering,Jiangnan University,Wuxi,Jiangsu 214122,China;
    2.Key Laboratory of Food Colloids and Biotechnology,Ministry of Education,Jiangnan University,Wuxi,Jiangsu 214122,China
  • Received:2016-12-29 Published:2019-03-20

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摘要： 通过优化透骨草提取工艺,再经有机溶剂萃取,得到透骨草有效抑菌活性成分(PAI)。结果表明,在乙醇体积分数80%,温度60 ℃,料液比1∶30 (g∶mL),时间4 h的条件下提取效果较优,经氯仿萃取后剩余水相有较强抑菌作用,对金黄色葡萄球菌和大肠杆菌的抑菌圈直径分别为(24.32±0.65)和(25.44±1.10) mm,其最小抑菌浓度(MIC)分别为6.25和3.12 g/L。此外,还通过细菌生长抑制曲线、细胞膜通透性以及细胞形貌观察等手段,研究PAI对细菌的抑制机理。结果表明,PAI在细菌繁殖的对数生长期起主要抑制作用,同时破坏细胞膜的通透性和细胞壁的结构,通过栅栏效应达到抑菌作用。

关键词: 透骨草提取物, 提取工艺, 最小抑菌浓度, 抑菌机理

Abstract: Technological conditions for extraction of antibacterial active ingredient in Phryma leptostachya L.by ethanol was optimized.Then the extract was further extracted by a water immiscible organic solvent chloroform to enrich the effective antibacterial active ingredient (PAI).Extraction under the condition of ethanol volume fraction 80%,60 ℃,ratio of solid to liquid 1∶30 (g∶mL) and 4 h shows the optimal results,and the remaining aqueous phase raffinate after extraction with chloroform shows strong antibacterial activity.The diameter of inhibition zone for S.aureus and E.coli are (24.32±0.65) and (25.44±1.10) mm respectively,and the minimum inhibition concentration (MIC) are 6.25 and 3.12 g/L respectively.In addition,the bacterial growth curve,cell membrane permeability and cell morphology observation are applied to examine the inhibition mechanism on S.aureus and E.coli.Results show that PAI mainly effects on the logarithmic growth phase and destroys the permeability of the cell membrane as well as the structure of the cell wall;thus the bacteria are inhibited by multiple-hurdle effect.

Key words: Phryma leptostachya L.extract, extraction process, minimum inhibition concentration, antibacterial mechanism

中图分类号: 

  • TQ047.6