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日用化学工业（中英文）

日用化学工业（中英文） ›› 2023, Vol. 53 ›› Issue (10): 1227-1232.doi: 10.3969/j.issn.2097-2806.2023.10.015

• 分析与检测 • 上一篇    下一篇

基于扩增子测序分析益生菌牙膏中菌群多样性

吴渊1,2,梁媛媛1,2,周志南1,2,黄甜甜1,2,夏琪琪1,2,刘鹏鹏1,2,*()   

  1. 1.浙江方圆检测集团股份有限公司，浙江 杭州 310020
    2.浙江省市场监管生物安全重点实验室，浙江 杭州 310020
  • 收稿日期:2022-10-07 修回日期:2023-09-28 出版日期:2023-10-22 发布日期:2023-10-27
  • 基金资助:
    浙江省市场监督管理局科研计划项目(ZC2021A029);浙江省市场监督“雏鹰计划”核心项目(CY2022229)

Analysis of bacterial diversity in probiotic toothpaste based on amplicon sequencing

Wu Yuan1,2,Liang Yuanyuan1,2,Zhou Zhinan1,2,Huang Tiantian1,2,Xia Qiqi1,2,Liu Pengpeng1,2,*()   

  1. 1. Zhejiang Fangyuan Testing Group Co., Ltd., Hangzhou, Zhejiang 310020, China
    2. Key Laboratory of Biosafety Detection for Zhejiang Market Regulation, Hangzhou, Zhejiang 310020, China
  • Received:2022-10-07 Revised:2023-09-28 Online:2023-10-22 Published:2023-10-27
  • Contact: *Tel.: +86-18858125369, E-mail: 410563321@qq.com.

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摘要：

通过常规方法检测益生菌牙膏中的活性菌，并采用高通量测序技术对20种市售益生菌牙膏16S rDNA的V3、V4区进行测序，扩增子测序分析不同牙膏中的菌群结构并进行α多样性分析，最后基于特异性引物，使用PCR方法检测20种牙膏中副干酪乳杆菌成分。结果表明：1）20种益生菌牙膏菌落总数均<10 CFU/g，乳酸菌总数均<10 CFU/g；2）通过扩增子测序结果分析，牙膏中还添加了干酪乳杆菌以外的乳酸菌，有18种牙膏中添加了发酵乳杆菌、嗜酸乳酸杆菌和德氏乳杆菌亚种，20种牙膏中添加了植物乳杆菌，19种牙膏中添加了罗伊氏乳杆菌。高通量测序对原核微生物的α多样性分析，表明牙膏中原核微生物丰度和菌落多样性较高；3）牙膏DNA提取物通过PCR及测序，结果表明20种牙膏均按标签标识添加了副干酪乳杆菌。

关键词: 益生菌牙膏, 扩增子测序, 副干酪乳杆菌

Abstract:

The active bacteria in 20 commercially available probiotic toothpastes were detected by conventional methods, and high-throughput sequencing of the V3 and V4 regions of 16S rDNA. The amplicon analysis was used for α Diversity and Microbial Community Structure analysis. Finally, based on the specific primers, the PCR method was used to detect Lactobacillus paracasei in 20 toothpastes. 1） The results show that the total bacterial count of 20 probiotic toothpastes is <10 CFU/g, and the total lactic acid bacteria is <10 CFU/g. 2） Through analyzing the amplicon PCR sequencing results, lactobacillus other than Lactobacillus casei are added to the toothpaste, 18 toothpastes add Lactobacillus fermentans, Lactobacillus acidophilus and Lactobacillus delbrueckii subspecies, 20 toothpastes add Lactobacillus plantarum, and 19 toothpastes add Lactobacillus reuteri. The sequencing diversity analysis show that the abundance of prokaryotic microorganisms and the diversity of colonies in the toothpaste are high. 3） The results of PCR and sequencing of toothpaste DNA extracts show that Lactobacillus paracasei is added to 20 toothpastes according to the label.

Key words: probiotic toothpaste, amplicon sequencing, Lactobacillus paracasei

中图分类号: 

  • TQ658