表没食子儿茶素没食子酸酯脂质体的制备及其透皮性能研究

doi:10.3969/j.issn.1001-1803.2020.09.005

摘要/Abstract

摘要：

采用大豆卵磷脂、胆固醇为壁材,通过薄膜-超声分散法制备了表没食子儿茶素没食子酸酯（EGCG）脂质体（EGCG Liposome,EL）,研究了胆固醇用量、水化温度、超声时间、EGCG添加量对EL粒径、PDI、包封率以及产率的影响。进一步对EL的透皮效果进行研究,并测定了其储藏稳定性。结果表明,在卵磷脂和胆固醇的质量比为1∶0.25,超声时间为6 min,水化温度为50 ℃,EGCG和卵磷脂的质量比为0.075∶1时,EL包封率、产率和粒径都较优,包封率和产率分别为84.75%±2.12%和93.78%±2.16%,粒径为（132.74±1.03） nm,PDI为0.245±0.009。通过透皮和储藏稳定性实验可知,与EGCG水溶液相比,脂质体能显著提高EGCG的透皮运输能力及储藏稳定性。

关键词: 表没食子儿茶素没食子酸酯脂质体, 制备, 透皮运输, 稳定性

Abstract:

Epigallocatechin gallate （EGCG） liposomes （EL） were prepared by membrane-ultrasonic dispersion using soybean lecithin and cholesterol as encapsulation materials. The effects of the amount of cholesterol, hydration temperature, ultrasonic time, EGCG addition amount on the particle size, PDI, entrapment efficiency and yield of EL were studied. Also, the transdermal performance and the storage stability of EL were measured. The results show that, when the mass ratio of lecithin to cholesterol is 1∶0.25, the ultrasonic time is 6 min, the hydration temperature is 50 ℃, and the mass ratio of EGCG to lecithin is 0.075∶1, the high encapsulation efficiency and yield are achieved as 84.75%±2.12% and 93.78%±2.16%, and the particle size is （132.74±1.03） nm, the PDI is 0.245±0.009. According to the storage stability and transdermal experiments, liposomes can improve the transdermal transport and stability of EGCG significantly compared with the EGCG aqueous solution.

Key words: epigallocatechin gallate liposomes, preparation, skin penetration, stability

中图分类号:

TQ658

曹振大,梁蓉. 表没食子儿茶素没食子酸酯脂质体的制备及其透皮性能研究[J]. 日用化学工业, 2020, 50(9): 609-614.

CAO Zhen-da,LIANG Rong. Study on the preparation and transdermal performance of epigallocatechin gallate liposome[J]. China Surfactant Detergent & Cosmetics, 2020, 50(9): 609-614.

图/表 6

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