0~2岁口周轻度湿疹患儿皮肤细菌多样性及润肤霜使用对菌群的影响

doi:10.3969/j.issn.1001-1803.2020.09.006

摘要/Abstract

摘要：

采用16SrRNA基因高通量测序技术,研究了0~2岁婴幼儿轻度湿疹皮肤与健康皮肤及使用润肤霜前后细菌组成差异。结果显示,相比健康组,湿疹组放线菌属、固氮螺菌属、红球菌属等显著性上升,而[Prevotella]、湖沉积杆菌属、农杆菌属和乳酸菌属等的丰度显著下降（P<0.05）。种水平上金黄色葡萄球菌、Hyphomicrobium zavarzinii、咽峡炎链球菌显著性升高,而溶血葡萄球菌、墨西哥假单胞菌等丰度显著性下降（P<0.05）。湿疹皮肤使用润肤剂1周后,临床症状有所改善,细菌OTUs数量增加,厌氧芽胞杆菌（Anoxybacillus kestanbolensis）、微球菌（藤黄微球菌）、Mycoplana等丰度显著升高,而不动杆菌、固氮螺菌属、生丝微菌属（Hyphomicrobium zavarzinii）、伯克霍尔德菌（Burkholderia bryophila）等显著降低（P<0.05）。结果表明轻度口周湿疹样皮肤细菌组成与健康皮肤不同,与金黄色葡萄球等的显著上升相关,润肤霜等保湿化妆品可以缓解轻度湿疹,与不动杆菌、金黄色葡萄球菌等致病菌的丰度减少相关。

关键词: 湿疹, 口周皮肤, 皮肤细菌, 润肤霜

Abstract:

16SrRNA gene high-throughput sequencing technique was used to study the difference of bacterial composition between mild eczema skin and healthy skin of 0-2 year-old children as well as before and after using emollient. It was found that compared with the healthy group, the abundance of Actinomycetes, Azospirillum and Rhodococcus increase significantly in eczema group, while the abundance of [Prevotella], Limnobacter, Agrobacterium and Lactobacillus decrease significantly （P<0.05）. At the species level, the abundance of Staphylococcus aureus （P<0.01）, Hyphomicrobium zavarzinii, and Streptococcus anginosus （P<0.05） are significantly higher, while the abundance of Staphylococcus haemolyticus （P<0.05） and Pseudoxanthomonas mexicana （P<0.001） are significantly lower. After the use of emollients for one week, the clinical symptoms of eczema are improved, and the number of bacteria operational taxonomic units （OTUs） shared by the healthy and post-treatment mild eczema groups increase compared with those shared by the healthy and mild eczema groups. Taxonomic composition analysis reveals that Anoxybacillus （Anoxybacillus kestanbolensis）, Micrococcus （Micrococcus luteus）, and Mycoplana levels increase significantly after applying emollients, while Actinomyces, Azospirillum, Hyphomicrobium （H.zavarzinii）, and Burkholderia （Burkholderia bryophila） decrease significantly （P<0.05）. The results show that the bacterial composition of mild perioral eczema-like skin is different from that of healthy skin, which is related to the significant increase of Staphylococcus aureus. Moisturizing cosmetics such as emollients can relieve mild eczema and it is related to the decrease of the abundance of pathogenic bacteria such as Actinomycetes and Staphylococcus aureus.

Key words: eczema, perioral skin, skin bacteria, emollients

中图分类号:

TQ658

郑玉梅,王茜,马来记,陈圆圆,高莹,宋丽雅. 0~2岁口周轻度湿疹患儿皮肤细菌多样性及润肤霜使用对菌群的影响[J]. 日用化学工业, 2020, 50(9): 615-619.

ZHENG Yu-mei,WANG Qian,MA Lai-ji,CHEN Yuan-yuan,GAO Ying,SONG Li-ya. Diversity of skin microbiome in children aged 0-2 years old with mild perioral eczema and the effects of emollients treatment on the microflora[J]. China Surfactant Detergent & Cosmetics, 2020, 50(9): 615-619.

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分组	总人数/人	平均年龄/月	性别（女）	生产方式（剖腹产）	父母过敏史
健康组（H组）	27	9.15±7.57	17	8	6例
湿疹组（L组）	22	11.93±8.45	10	8	4例