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2020年, 第50卷, 第9期　刊出日期：2020-09-22 上一期    下一期

基础研究

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十二醇邻苯二甲酸单酯钠的合成与性能研究 Synthesis and properties of sodium dodecyl phthalate monoester 张志东,方银军,刘雪锋 2020 (9):  581-587.  doi: 10.3969/j.issn.1001-1803.2020.09.001 摘要 ( 279 )   HTML ( 87 )   PDF(1421KB) ( 460 )   采用十二醇和邻苯二甲酸酐经酯化和中和反应得到十二醇邻苯二甲酸单酯钠（PAS12）,并通过 1H NMR确定了其分子结构。PAS12的Krafft温度为16.4 ℃,与十二酸钠（SL,15.5 ℃）相近,明显低于十六酸钠（SP,57.4 ℃）;与SL相比,cmc（PAS12） ＜ cmc（SL）,γcmc（PAS12） ＜ γcmc（SL）,σcmc（PAS12） ＜ σcmc（SL）,PAS12的乳液稳定性、耐受Ca 2+、发泡力和泡沫稳定性更好,其原因可能是PAS12分子结构中位于亲水基（-COONa）和疏水基（C12H25-）之间的苯环和酯键片段增加了PAS12疏水基的有效长度,且苯环和酯键片段同时兼具部分亲水性和疏水性,为PAS12分子本身的极性从强极性（-COONa）向弱极性（C12H25-）转换提供过渡,有利于PAS12有效地降低气-液和液-液界面张力;再加上苯环上二羧酸邻位取代结构使得PAS12更好地耐受Ca 2+。因此,PAS12具有较好的低温水溶性、较低的表（界）面张力、良好的乳化性能、泡沫性能和一定的耐硬水性质。 Abstract: Sodium dodecyl phthalate monoester （PAS12） was synthesized by esterification of dodecyl alcohol and phthalic anhydride and then neutralization, and its molecular structure was confirmed by 1H NMR. The Krafft temperature of PAS12 was 16.4 ℃, which was similar to sodium laurate （SL, 15.5 ℃） and significantly lower than that of sodium palmitate （SP, 57.4 ℃）. Compared with SL, results of cmc（PAS12） ＜ cmc（SL）, γcmc（PAS12） ＜ γcmc （SL）, σcmc（PAS12） ＜ σcmc（SL）, better emulsion stability, Ca 2+-tolerance to foaming performance and foam stability for PAS12 were observed. The segment of benzene ring and ester between the hydrophilic group （-COONa） and the hydrophobic group （C12H25-） might increase the effective length of hydrophobic chain. And this segment had both partial hydrophilicity and hydrophobicity, which could provide a transition from strong polarity （-COONa） to weak polarity （C12H25-） for the PAS12. It was therefore beneficial for PAS12 to reduce gas-liquid and liquid-liquid interfacial tensions; furthermore the structure of the ortho-substituted benzene ring might give PAS12 a better Ca 2+-tolerance. Therefore, PAS12 exhibited better low-temperature water solubility, lower surface/interfacial tension, better emulsifying properties, foam properties and hard water resistance. 数据和表 | 参考文献 | 相关文章 | 计量指标

科技讲座

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两亲聚合物设计合成及其增效体系研究(IX)— 流变性能 Study on the design and synthesis of amphiphilic polymers and their synergistic systems (IX)Rheological properties 康万利,何瑛琦,杨红斌,王芳,Bauyrzhan Sarsenbekuly 2020 (9):  588-595.  doi: 10.3969/j.issn.1001-1803.2020.09.002 摘要 ( 256 )   HTML ( 8 )   PDF(1069KB) ( 438 )   综述了两亲聚合物及其增效体系的流变特性的研究进展。首先概述了两亲聚合物的流变特性,总结了其黏浓关系、稳态与动态剪切条件下的流变性能以及流变模式。其次探讨了温度、矿化度和时间对两亲聚合物流变性能的影响规律。最后对3种增效体系,即两亲聚合物静电作用增效体系、两亲聚合物疏水缔合作用增效体系和两亲聚合物主客体包合作用增效体系的流变特性进行了阐述。 The rheological properties of amphiphilic polymers and their synergistic systems are reviewed. Firstly, the rheological properties of amphiphilic polymers are summarized, including the viscosity relationship, rheological properties under steady and dynamic shear conditions and rheological model. Secondly, the effects of temperature, salinity and time on the rheological properties of amphiphilic polymers are discussed. Finally, the rheological properties of three synergetic systems containing amphiphilic polymers are described, which are electrostatic synergetic system, hydrophobic associative synergetic system and host/guest package synergetic system. 数据和表 | 参考文献 | 相关文章 | 计量指标

开发与应用

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N-酰基氨基酸型表面活性剂与牛血清蛋白相互作用研究 Study on the interaction between N-acyl amino acid surfactants and bovine serum albumi 惠蒙蒙,张晨龙,杨许召,白亚榕,王军 2020 (9):  596-602.  doi: 10.3969/j.issn.1001-1803.2020.09.003 摘要 ( 356 )   HTML ( 9 )   PDF(1326KB) ( 463 )   通过荧光光谱和紫外吸收光谱研究了牛血清蛋白（BSA）与N-月桂酰基肌氨酸钠（SLS）、N-月桂酰基谷氨酸钠（SLGLU）和N-月桂酰基甘氨酸钠（SLG） 3种N-酰基氨基酸型表面活性剂之间的相互作用。随着3种表面活性剂浓度增加,BSA中色氨酸残基的内源荧光光谱波长都发生了蓝移,色氨酸残基所处微环境极性减弱。荧光探针法测得3种表面活性剂在蛋白质表面上形成聚集体所需的临界浓度（cac）分别为：cacSLS=0.4 mmol/L,cacSLG=0.1 mmol/L,cacSLGLU=0.09 mmol/L。BSA与3种表面活性剂相互作用的结合等温线基本上都可以划分为特异性结合、非协同结合、协同结合和饱和4个特征区域。随着表面活性剂浓度增大,BSA中色氨酸残基的紫外特征吸收波长红移,氨基酸残基所处微环境极性减弱,而酪氨酸和苯丙氨酸残基的紫外特征吸收波长蓝移,氨基酸残基所处微环境极性增强。利用苯基丁氮酮、布洛芬、氯化血红素探针分析,确定了3种表面活性剂与BSA相互作用时的结合位点为BSA结构域II A的Trp-213附近位置,并对不同表面活性剂浓度时AAS与BSA相互作用方式进行了分析。 The interaction between bovine serum albumi （BSA） and three N-acyl amino acid surfactants, including sodium lauroyl sarcosinate （SLS）, sodium lauroyl glutamate （SLGLU） and sodium lauroyl glycine （SLG） was studied by fluorescence spectrum and UV spectrum in this paper. The fluorescence peak wavelength of tryptophan residue in BSA showed a blue-shift with the increase of the concentration of the three surfactants, and the polarity of microenvironment around the tryptophan residue was decreased. The critical aggregation concentrations （cac） of the three surfactants on protein surface of BSA by fluorescence spectrum are: cacSLS=0.4 mmol/L, cacSLG=0.1 mmol/L, and cacSLGLU=0.09 mmol/L, respectively. The binding isotherms of BSA interacted with the three surfactants can be divided into four feature regions: specific binding, non-cooperative binding, cooperative binding and saturation. With the increase of surfactant concentration, the characteristic UV absorption wavelength of tryptophan residue in BSA is red shifted, and the polarity of amino acid residues in the microenvironment is decreased; while the characteristic UV absorption wavelengths of tyrosine and phenylalanine residues in BSA are blue-shift, and the polarity of amino acid residues in BSA is enhanced. The binding site of BSA with the three surfactants was confirmed at the vicinity of Trp-213 of BSA domain II A by probe molecules, such as phenylbutazone, ibuprofen and heme chloride. The interaction modes between AAS and BSA at different surfactant concentrations were analyzed. 数据和表 | 参考文献 | 相关文章 | 计量指标

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棕榈酸木薯淀粉酯的制备及性能研究 Preparation and properties of palmitate cassava starch 韦爱芬,于斌,韦莉敏 2020 (9):  603-608.  doi: 10.3969/j.issn.1001-1803.2020.09.004 摘要 ( 286 )   HTML ( 6 )   PDF(993KB) ( 334 )   以木薯淀粉为原料,棕榈酸为酯化剂,硅酸钠和醋酸为复合催化剂,采用干法工艺制备棕榈酸木薯淀粉酯。研究了棕榈酸用量、催化剂用量、反应时间、反应温度对淀粉酯取代度和表面张力的影响,研究了淀粉酯取代度与表面活性及乳化性能之间的关系。取代度的测试结果及红外光谱均表明淀粉已被成功酯化。当木薯淀粉用量为100 g,棕榈酸用量为15 g,复合催化剂(硅酸钠与醋酸质量比为5∶1)用量为7.5 g,反应温度为160 ℃,反应时间为4 h时,制得的棕榈酸木薯淀粉酯取代度(DS)为0.012 3,棕榈酸含量为2.39%(w/w),临界胶束浓度为30 g/L,对应的表面张力为25.87 mN/m,具有较好的表面活性和乳化性能。 Using cassava starch as raw material, palmitic acid as esterification agent, sodium silicate and acetic acid as composite catalysts, palmitate cassava starch was prepared by dry process. The effects of palmitate, catalyst, reaction time and reaction temperature on the degree of substitution and surface tension of starch ester were studied. The relationship between the degree of substitution of palmitate cassava starch and its surface activity and emulsifying property was studied. The results of the degree of substitution and infrared spectrum show that the starch has been esterified by palmitate acid. The optimum reaction conditions are as follows: the amount of cassava starch is 100 g, the amount of palmitic acid is 15 g, the amount of composite catalyst (the mass ratio of sodium silicate to acetic acid is 5∶1) is 7.5 g, the reaction temperature is 160 ℃, and the reaction time is 4 h. The DS of the product is 0.012 3, its palmitate content is 2.39% (w/w), the critical micelle concentration is 30 g/L, and its surface tension is 25.87 mN/m. The test results indicate that the palmitate cassava starch has good surface activity and emulsifying property. 数据和表 | 参考文献 | 相关文章 | 计量指标

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表没食子儿茶素没食子酸酯脂质体的制备及其透皮性能研究 Study on the preparation and transdermal performance of epigallocatechin gallate liposome 曹振大,梁蓉 2020 (9):  609-614.  doi: 10.3969/j.issn.1001-1803.2020.09.005 摘要 ( 252 )   HTML ( 16 )   PDF(1013KB) ( 422 )   采用大豆卵磷脂、胆固醇为壁材,通过薄膜-超声分散法制备了表没食子儿茶素没食子酸酯（EGCG）脂质体（EGCG Liposome,EL）,研究了胆固醇用量、水化温度、超声时间、EGCG添加量对EL粒径、PDI、包封率以及产率的影响。进一步对EL的透皮效果进行研究,并测定了其储藏稳定性。结果表明,在卵磷脂和胆固醇的质量比为1∶0.25,超声时间为6 min,水化温度为50 ℃,EGCG和卵磷脂的质量比为0.075∶1时,EL包封率、产率和粒径都较优,包封率和产率分别为84.75%±2.12%和93.78%±2.16%,粒径为（132.74±1.03） nm,PDI为0.245±0.009。通过透皮和储藏稳定性实验可知,与EGCG水溶液相比,脂质体能显著提高EGCG的透皮运输能力及储藏稳定性。 Epigallocatechin gallate （EGCG） liposomes （EL） were prepared by membrane-ultrasonic dispersion using soybean lecithin and cholesterol as encapsulation materials. The effects of the amount of cholesterol, hydration temperature, ultrasonic time, EGCG addition amount on the particle size, PDI, entrapment efficiency and yield of EL were studied. Also, the transdermal performance and the storage stability of EL were measured. The results show that, when the mass ratio of lecithin to cholesterol is 1∶0.25, the ultrasonic time is 6 min, the hydration temperature is 50 ℃, and the mass ratio of EGCG to lecithin is 0.075∶1, the high encapsulation efficiency and yield are achieved as 84.75%±2.12% and 93.78%±2.16%, and the particle size is （132.74±1.03） nm, the PDI is 0.245±0.009. According to the storage stability and transdermal experiments, liposomes can improve the transdermal transport and stability of EGCG significantly compared with the EGCG aqueous solution. 数据和表 | 参考文献 | 相关文章 | 计量指标

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0~2岁口周轻度湿疹患儿皮肤细菌多样性及润肤霜使用对菌群的影响 Diversity of skin microbiome in children aged 0-2 years old with mild perioral eczema and the effects of emollients treatment on the microflora 郑玉梅,王茜,马来记,陈圆圆,高莹,宋丽雅 2020 (9):  615-619.  doi: 10.3969/j.issn.1001-1803.2020.09.006 摘要 ( 428 )   HTML ( 7 )   PDF(1163KB) ( 518 )   采用16SrRNA基因高通量测序技术,研究了0~2岁婴幼儿轻度湿疹皮肤与健康皮肤及使用润肤霜前后细菌组成差异。结果显示,相比健康组,湿疹组放线菌属、固氮螺菌属、红球菌属等显著性上升,而[Prevotella]、湖沉积杆菌属、农杆菌属和乳酸菌属等的丰度显著下降（P<0.05）。种水平上金黄色葡萄球菌、Hyphomicrobium zavarzinii、咽峡炎链球菌显著性升高,而溶血葡萄球菌、墨西哥假单胞菌等丰度显著性下降（P<0.05）。湿疹皮肤使用润肤剂1周后,临床症状有所改善,细菌OTUs数量增加,厌氧芽胞杆菌（Anoxybacillus kestanbolensis）、微球菌（藤黄微球菌）、Mycoplana等丰度显著升高,而不动杆菌、固氮螺菌属、生丝微菌属（Hyphomicrobium zavarzinii）、伯克霍尔德菌（Burkholderia bryophila）等显著降低（P<0.05）。结果表明轻度口周湿疹样皮肤细菌组成与健康皮肤不同,与金黄色葡萄球等的显著上升相关,润肤霜等保湿化妆品可以缓解轻度湿疹,与不动杆菌、金黄色葡萄球菌等致病菌的丰度减少相关。 16SrRNA gene high-throughput sequencing technique was used to study the difference of bacterial composition between mild eczema skin and healthy skin of 0-2 year-old children as well as before and after using emollient. It was found that compared with the healthy group, the abundance of Actinomycetes, Azospirillum and Rhodococcus increase significantly in eczema group, while the abundance of [Prevotella], Limnobacter, Agrobacterium and Lactobacillus decrease significantly （P<0.05）. At the species level, the abundance of Staphylococcus aureus （P<0.01）, Hyphomicrobium zavarzinii, and Streptococcus anginosus （P<0.05） are significantly higher, while the abundance of Staphylococcus haemolyticus （P<0.05） and Pseudoxanthomonas mexicana （P<0.001） are significantly lower. After the use of emollients for one week, the clinical symptoms of eczema are improved, and the number of bacteria operational taxonomic units （OTUs） shared by the healthy and post-treatment mild eczema groups increase compared with those shared by the healthy and mild eczema groups. Taxonomic composition analysis reveals that Anoxybacillus （Anoxybacillus kestanbolensis）, Micrococcus （Micrococcus luteus）, and Mycoplana levels increase significantly after applying emollients, while Actinomyces, Azospirillum, Hyphomicrobium （H.zavarzinii）, and Burkholderia （Burkholderia bryophila） decrease significantly （P<0.05）. The results show that the bacterial composition of mild perioral eczema-like skin is different from that of healthy skin, which is related to the significant increase of Staphylococcus aureus. Moisturizing cosmetics such as emollients can relieve mild eczema and it is related to the decrease of the abundance of pathogenic bacteria such as Actinomycetes and Staphylococcus aureus. 数据和表 | 参考文献 | 相关文章 | 计量指标

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无水牙膏增稠剂甘油溶液的流变特性研究 Study on the rheological properties of glycerin solution of anhydrous toothpaste thickener 徐春生,陈丽娜,刘东辉 2020 (9):  620-623.  doi: 10.3969/j.issn.1001-1803.2020.09.007 摘要 ( 447 )   HTML ( 9 )   PDF(1150KB) ( 496 )   采用DV3T型流变仪测试无水牙膏增稠剂羟丙基决明胶、卡波姆的甘油溶液流变曲线,通过幂律方程拟合分析了相关的稠度系数、假塑性因子和黏度。试验数据显示,1.6%羟丙基决明胶与0.9%~1.0%卡波姆956的流变性能比较符合无水牙膏配方的要求。 DV3T rheometer was used to test the rheological curve of the glycerine solution of hydroxypropyl gelatine and carbomer. The relevant consistency coefficient, pseudoplastic factor and viscosity were analyzed by power-law equation fitting. The test data show that the rheological properties of 1.6% hydroxypropyl gelatine and 0.9%-1.0% carbomer 956 meet the requirements of anhydrous toothpaste formulation. 数据和表 | 参考文献 | 相关文章 | 计量指标

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阿魏酰氨基酸乙酯的合成及其抗氧化活性研究 Synthesis and anti-oxidant activity of feruloylamino acid ethyl ester 郭晓丹,宋京九,祝钧 2020 (9):  624-628.  doi: 10.3969/j.issn.1001-1803.2020.09.008 摘要 ( 309 )   HTML ( 9 )   PDF(956KB) ( 412 )   以阿魏酸和氨基酸乙酯盐酸盐为原料,1-（3-二甲基氨基丙基）-3-乙基碳二亚胺盐酸盐（EDC·HCl）和1-羟基苯并三唑（HOBt）为催化剂,采用EDC缩合法合成阿魏酰蛋氨酸乙酯（Ⅰ）、阿魏酰异亮氨酸乙酯（Ⅱ）、阿魏酰苏氨酸乙酯（Ⅲ）和阿魏酰苯丙氨酸乙酯（Ⅳ）。利用核磁共振氢谱（ 1H NMR）和高效液相色谱-质谱（HPLC-MS）对产物结构进行表征,并且比较了阿魏酰氨基酸乙酯和阿魏酸在清除DPPH自由基和ABTS自由基方面的活性。结果表明：合成的4种阿魏酰氨基酸乙酯能够较好的清除自由基,且清除率均大于阿魏酸单体;阿魏酰苯丙氨酸乙酯（Ⅳ）清除DPPH自由基和ABTS自由基能力最强,IC50分别为37.567和1.689 μmol/L。红细胞溶血实验表明：合成的阿魏酰氨基酸衍生物Ⅰ、Ⅲ、Ⅳ的刺激性均小于阿魏酸。 Ferulic methionine ethyl ester （Ⅰ）, feruli isoleucine ethyl ester （Ⅱ）, feruli threonine ethyl ester （Ⅲ） and feruli phenylalanine ethyl ester （Ⅳ） were synthesized by EDC condensation using ferulic acid and amino acid ethyl ester hydrochloride as raw materials, and 1-（3-dimethylaminopropyl）-3-ethylcarbodiimide hydrochloride （EDC·HCl） and 1-hydroxybenzotriazole （HOBt） as catalysts. The structure of the products was characterized by 1H NMR and HPLC-MS, and the activities of ferulic acid and ethyl ferulic acid in scavenging DPPH and ABTS free radicals were compared. The results show that the four kinds of ferulic acid ethyl ester scavenged free radicals better, and the scavenging rates are higher than that of ferulic acid monomer. The ferryl phenylalanine ethyl ester （Ⅳ） has the strongest ability to scavenge DPPH free radicals and ABTS free radicals, with IC50 of 37.567 and 1.689 μmol/L, respectively. The red blood cells hemolysis test show that the irritation of the synthesized derivatives of ferulic acid Ⅰ, Ⅲ and Ⅳ is less than that of ferulic acid. 数据和表 | 参考文献 | 相关文章 | 计量指标

专论与综述

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化妆品中性激素的检测方法研究进展 Research progress on detection of sex hormones in cosmetics 胡贝,李丽霞,刘红,黄伟,丁晓萍 2020 (9):  629-637.  doi: 10.3969/j.issn.1001-1803.2020.09.009 摘要 ( 359 )   HTML ( 10 )   PDF(843KB) ( 324 )   概述了化妆品中性激素的种类、安全风险,介绍目前检测标准和文献所涵盖的性激素种类。综述了高效液相色谱法、超高效液相色谱法、液相色谱-质谱法及凝胶渗透色谱-液相色谱-串联质谱法、超高效合相色谱法、聚合物整体柱微萃取与高效液相色谱联用法、固相萃取-微乳液相色谱法等检测方法在性激素检测上的应用及其优劣。最后对化妆品中性激素检测技术的发展趋势进行了介绍。 The categories and safety risks of sex hormones in cosmetics were presented. The detection standards and the cutting-edge methods reported referring to these categories of sex hormones in cosmetics were also introduced. The advantages and disadvantages of various detection methods were introduced, including HPLC, UPLC, HPLC-MS and other detection methods （GPC-LC-MS/MS, UPC2, PMME-HPLC, and SPE-MELC）. Finally, the development trend of detection methods for sex hormones in cosmetics was mentioned. 数据和表 | 参考文献 | 相关文章 | 计量指标

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敏感性头皮与微生态屏障 Sensitive scalp and microecologic barrier 任慧,汤小芹,陈明华 2020 (9):  638-642.  doi: 10.3969/j.issn.1001-1803.2020.09.010 摘要 ( 565 )   HTML ( 26 )   PDF(844KB) ( 1632 )   概述了近年来国内外关于敏感头皮及其微生态屏障的研究,包括头皮上常见微生物的分布,微生物和头皮的相互作用,微生态屏障对敏感性头皮的重要性。讨论了头皮微生物群落对头皮的积极以及负面作用的发生途径,分析了敏感性头皮常见症状,以及其对应现象下头皮微生物分布的变化。 This article reviews the recent studies on sensitive scalp and its relationship with microecological barriers, including the distribution of common microorganisms on the scalp, the interaction between microorganisms and scalp, and the importance of microecological barrier on sensitive scalp. This article discusses the pathway of the positive and negative effects of the microbial community on the scalp, and analyses the common symptoms of sensitive scalp and the corresponding changes of scalp microbiota. 数据和表 | 参考文献 | 相关文章 | 计量指标

分析与检测

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高效液相色谱-三重四极杆质谱法测定化妆品中的西咪替丁及雷尼替丁 Determination of cimetidine and ranitidine in cosmetics by high performance liquid chromatography-tandem mass spectrometry 王聪,李莉,王海燕,孙磊 2020 (9):  643-647.  doi: 10.3969/j.issn.1001-1803.2020.09.011 摘要 ( 301 )   HTML ( 5 )   PDF(1110KB) ( 412 )   建立可同时测定化妆品中西咪替丁及雷尼替丁的高效液相色谱-三重四极杆质谱方法。待测样品经甲醇超声提取,经0.2 μm滤膜过滤后进样检测,在优化的色谱、质谱条件下,经ZORBAX SB-Aq色谱柱分离。采用电喷雾离子源在正离子模式下进行多反应监测。用基质匹配外标曲线法进行定量分析。结果表明,西咪替丁、雷尼替丁的检出浓度分别为0.03和0.02 ng/g,在1~100 ng/mL范围内线性关系良好（r >0.999）,低、中、高3个质量浓度水平下的样品加标回收率为84.9%~116.2%,RSD不超过5%。建立的方法前处理过程简单,灵敏度、线性、回收率及重复性均满足方法学要求,可同时对化妆品中的西咪替丁和雷尼替丁进行测定。 A high performance liquid chromatography-tandem mass spectrometric method was established for determination of cimetidine and ranitidine in cosmetics. Samples were extracted by methanol ultrasonically, and then filtered through a 0.2 μm membrane. After separated by a ZORBAX SB-Aq column, samples were detected by MRM with electrospray ion source in positive mode. Matrix-matched external standard calibration curves were used for quantitative analysis. The concentration limits of detection of cimetidine and ranitidine were 0.03 and 0.02 ng/g, respectively. In the mass concentration range of 1-100 ng/mL, cimetidine and ranitidine showed good linear relationship with correlation coefficients all above 0.999．The recoveries for cimetidine and ranitidine at three spiked levels were in the range of 84.9%-116.2%, and the relative standard deviations of their recoveries were not more than 5%. This method was able to be applied in determination of cimetidine and ranitidine in cosmetics with simple pretreatment, good linearity and high recovery. 数据和表 | 参考文献 | 相关文章 | 计量指标

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离子色谱法和高效液相色谱法测定化妆品中的丁卡因及其盐类 Determination of tetracaine and its salts in cosmetics by IC and HPLC 郭项雨,赵卫哲,王锋,夏德富,李文涛,马强 2020 (9):  648-652.  doi: 10.3969/j.issn.1001-1803.2020.09.012 摘要 ( 375 )   HTML ( 7 )   PDF(1107KB) ( 344 )   建立了化妆品中丁卡因及其盐类的离子色谱和高效液相色谱分析方法,并对两种检测方法进行了比对分析。采用离子色谱法,丁卡因及其盐类的检出限和定量限分别为10和25 mg/kg,方法平均回收率为87.6%~102.7%,相对标准偏差为1.1%~4.7%。采用高效液相色谱法,丁卡因及其盐类的检出限和定量限分别为2和5 mg/kg,方法平均回收率为89.5%~105.6%,相对标准偏差为1.4%~5.8%。对于疑似阳性样品,进一步采用高效液相色谱-串联质谱法进行确证分析。经比较,两种方法检测化妆品中的丁卡因及其盐类的色谱保留时间均在5 min以内,方法学数据均满足分析要求。 Two analytical methods were developed for the determination of tetracaine and its salts in cosmetics based on ion chromatography （IC） and high-performance liquid chromatography （HPLC）. The two detection methods were compared. The limit of detection （LOD） and quantitation （LOQ） of tetracaine were 10 and 25 mg/kg for the IC method. The average recoveries ranged from 87.6% to 102.7% with the relative standard deviations （RSDs） of 1.1%-4.7%. The LOD and LOQ were 2 and 5 mg/kg for the HPLC method. The average recoveries ranged from 89.5% to 105.6% with the RSDs of 1.4%-5.8%. The suspected positive samples were subjected to further verification by high-performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）. The chromatographic retention time of tetracaine and its salts was both within 5 min and the experimental data of the two methods met the performance requirements. 数据和表 | 参考文献 | 相关文章 | 计量指标