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日用化学工业 ›› 2020, Vol. 50 ›› Issue (5): 309-313.doi: 10.3969/j.issn.1001-1803.2020.05.005

• 开发与应用 • 上一篇    下一篇

氨基酸型复合纳米乳抑菌性能的研究

赵志娟1,2,公培龙3,崔晓东1,王转花1()   

  1. 1. 山西大学生物技术研究所 化学生物学与分子工程教育部重点实验室,山西 太原 030006
    2. 山西医科大学 药学院,山西 太原 030001
    3. 中国日用化学研究院有限公司,山西 太原 030001
  • 收稿日期:2020-03-13 修回日期:2020-04-29 出版日期:2020-05-22 发布日期:2020-05-21
  • 通讯作者: 王转花
  • 作者简介:赵志娟(1980-),女,博士研究生,电话:13934511829,E-mail: zhaozhijuan82@hotmail.com。
  • 基金资助:
    山西省应用基础研究计划项目(201801D121192)

Bacteriostatic properties of amino acid composite nanoemulsion

ZHAO Zhi-juan1,2,GONG Pei-long3,CUI Xiao-dong1,WANG Zhuan-hua1()   

  1. 1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, Shanxi 030006, China
    2. Department of Pharmacy, Shanxi Medical University, Taiyuan, Shanxi 030001, China
    3. China Research Institute of Daily Chemical Co., Ltd., Taiyuan, Shanxi 030001, China
  • Received:2020-03-13 Revised:2020-04-29 Online:2020-05-22 Published:2020-05-21
  • Contact: Zhuan-hua WANG

摘要:

以氨基酸型表面活性剂月桂酰肌氨酸钠与PEG-40氢化蓖麻油为复合乳化剂,1,2-丙二醇为助乳化剂,蓖麻油为油相,构建荞麦黄酮纳米乳体系,并对其抑菌活性进行评价。结果表明,纳米乳外观透明澄清,粒径分布均匀,其均匀分散指数(PDI)、表面张力、pH值等均表明形成的纳米乳体系良好。体外释放性能显示24 h内,纳米乳累积释放量明显优于对照混悬液。纳米乳及混悬液对金黄色葡萄球菌和大肠杆菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)测定结果表明,纳米乳的抑菌活性明显高于混悬液。悬液定量法测定纳米乳对金黄色葡萄球菌、大肠杆菌的抑菌率分别为92.1%±1.93%和93.2%±1.26%;同浓度混悬液对金黄色葡萄球菌、大肠杆菌的抑菌率分别为42.0%±1.58%和45.6%±2.33%。

关键词: 氨基酸表面活性剂, 纳米乳, 体外释放性, 抑菌性

Abstract:

The buckwheat flavone nanoemulsion system was constructed with amino acid surfactant sodium lauroyl sarcosinate and PEG-40 hydrogenated castor oil as the composit emulsifiers, 1,2-propylene glycol as co-emulsifier and castor oil as oil phase, and its bacteriostatic activity was evaluated. The results showed that the nanoemulsion was transparent and clear in appearance, with uniform particle size distribution. The PDI, surface tension and pH value all showed that the nanoemulsion system was good. In vitro release performance showed that the cumulative release of nanoemulsion was significantly better than that of control suspension within 24 h. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of nanoemulsion and suspension to Staphylococcus aureus and Escherichia coli showed that the bacteriostatic activity of nanoemulsion was significantly higher than that of suspension. The bacteriostatic rates of nanoemulsion against Staphylococcus aureus and Escherichia coli were 92.1%±1.93% and 93.2%±1.26%, respectively, and those of the suspension of the same concentration against Staphylococcus aureus and Escherichia coli were 42.0%±1.58% and 45.6%±2.33%, respectively.

Key words: amino acid surfactant, nanoemulsion, in vitro release, bacteriostatic properties

中图分类号: 

  • TQ423