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日用化学工业（中英文）

日用化学工业 ›› 2017, Vol. 47 ›› Issue (11): 655-658.doi: 10.13218/j.cnki.csdc.2017.11.012

• 分析与检测 • 上一篇    下一篇

高效液相色谱法检测氨基酸表面活性剂中脂肪酸的含量

李泽勇, 陈磊, 刘康, 汤仲标, 张利萍   

  1. 广州天赐高新材料股份有限公司,广东 广州 510700
  • 收稿日期:2017-07-11 出版日期:2017-11-22 发布日期:2019-03-21
  • 作者简介:李泽勇(1986-),男,重庆人,博士,电话:18824911846,E-mail:lizeyong@tinci.com。
  • 基金资助:
    广州市产学研协同创新重大专项(201604016008)

Detection of content of fatty acids in amino acid-based surfactants by
high performance liquid chromatography

LI Ze-yong, CHEN Lei, LIU Kang, TANG Zhong-biao, ZHANG Li-ping   

  1. Guangzhou Tinci Materials Technology Co.,Ltd.,Guangzhou,Guangdong 510700,China
  • Received:2017-07-11 Online:2017-11-22 Published:2019-03-21

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摘要： 建立了一种快速检测氨基酸表面活性剂中脂肪酸残留的高效液相色谱分析方法。采用Welch Ultimate AQ-C18色谱柱(250 mm×4.6 mm,5 μm)分离,以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,样品经过酸化、衍生化处理后用紫外可见可变波长检测器(VWD)检测。结果表明,该方法的线性范围为5~500 mg/L,相关系数均大于0.999,平均回收率为93.2%~103.0%,检出限为0.03%,定量限为0.1%。方法耗时短、操作简单、准确度高、通用性强,适用于氨基酸表面活性剂产品的脂肪酸残留检测。

关键词: 氨基酸表面活性剂, 高效液相色谱, 脂肪酸

Abstract: A high performance liquid chromatography (HPLC) method for the detection of content of fatty acids in amino acid-based surfactants was established.Separation is performed by a Welch Ultimate AQ-C18 column (250 mm×4.6 mm,5 μm) with acetonitrile-0.1% formic acid aqueous solution as the mobile phase for gradient elution.After acidification and derivatization,the sample is detected with a UV and visible light wavelength variable detector (VWD).Results show that,all linearity ranges of the fatty acids are within 5-500 mg/L with the correlation coefficients greater than 0.999.The spiked recoveries vary from 93.2%-103.0%.The limits of determination and quantification are 0.03% and 0.1%,respectively.The method is simple,time-saving and precise,which may be suitable for fatty acids determination in amino acid-based surfactants.

Key words: amino acid-based surfactant, high performance liquid chromatography, fatty acids

中图分类号: 

  • TQ423