Abstract:

A solid phase extraction- high performance liquid chromatographic（HPLC） method was developed for the simultaneous determination of 15 caine-type prohibited ingredients in cosmetics. The samples were extracted by different solvent, then purified by solid phase extraction. Chromatographic separation was achieved on an C₁₈ chromatographic column （250 mm×4.6 mm×5 μm）. The column temperature was 30 ℃ through gradient elution with methanol-aqueous solution of 0.01 moL/L Na₂HPO₄ （pH 7.0） as binary mobile phase at a flow rate of 1.0 mL/min, and the detection wavelength was 230 nm. The standard curve method was used for quantification. The results show that the linear correlation coefficients of the 15 compounds are all greater than 0.999. The LODs of 15 caine-type prohibited ingredients are in the range of 1-13 mg/kg, while the LOQs are in the range of 3-29 mg/kg. The average recoveries at two spiked levels of astringent, milk and oil matrix are in the range of 90.9%-108.0%, 87.5%-104.5% and 87.7%-105.0% respectively, with RSDs of 0.1%-3.6%, 1.1%-8.8% and 0.5%-6.0% respectively. This method is proved to be efficient, accurate and durable, which provids an effective detection technique for 15 caine-type prohibited ingredients in cosmetics

Key words: cosmetics, caine-type local anesthetics, high performance liquid chromatography, solid phase extraction