馨肤白/壳聚糖皮克林乳液的制备及美白活性评价

doi:10.3969/j.issn.2097-2806.2025.11.002

Abstract

Abstract:

Chitosan nanoparticles （（212.2±3.2） nm） were synthesized via sol-gel method by adjusting the pH of the chitosan solution. Using polydimethylsiloxane （PDMS） as the oil phase, a chitosan-based Pickering emulsion （CS-PE） was prepared by high-speed shear homogenization, with solid particle mass fraction of 0.4% and water-to-oil volume ratio of 4∶4. Phenylethyl resorcinol （PR） was subsequently loaded into the emulsion to form the PR-loaded CS-PE （PR CS-PE）. The encapsulation efficiency and drug loading of PR in PR CS-PE were determined to be 97.2%±0.2% and 2.3%±0.01%, respectively. The radical scavenging rates of PR CS-PE for 2, 2-diphenyl-1-picrylhydrazyl （DPPH） and 2, 2′-azinobis（3-ethylbenzothiazoline-6-sulfonic acid）（ABTS） were measured to be 77.4% and 93.8%, respectively, indicative of significant antioxidant activity. The results of Franz diffusion cell test indicated that the PR within the emulsion exhibited sustained release behavior. Furthermore, PR CS-PE could effectively inhibit the melanogenesis in zebrafish models, suggesting notable skin-whitening efficacy. Results of the zebrafish embryonic development and cytotoxicity assay indicated that the PR-loaded chitosan-based Pickering emulsion exhibited lower toxicity compared with free PR. These findings suggest the potential application of the PR/chitosan Pickering emulsion in cosmetics.

Key words: chitosan nanoparticle, Pickering emulsion, phenylethyl resorcinol, zebrafish, cytotoxicity

CLC Number:

O648.2

Qiusi Yan, Hui Bi, Wenxin Dong, Aihua Zou. Preparation and skin-whitening activity evaluation of phenylethyl resorcinol/chitosan Pickering emulsion[J].China Surfactant Detergent & Cosmetics, 2025, 55(11): 1378-1387.

Figures/Tables 12

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References 43

[1]	Zhang Y, Sil B C, Kung C P, et al. Characterization and topical delivery of phenylethyl resorcinol[J]. Int J Cosmet Sci, 2019, 41 (5) : 479-488. doi: 10.1111/ics.v41.5
[2]	Schmaus G, Vielhaber G, Jacobs K, et al. 4- (1-phenylethyl) 1, 3-benzenediol: A new highly potent lightening agent[J]. J Cosmet Sci, 2006, 57 (2) : 197-198.
[3]	Sorg O, Kasraee B, Salomon D, et al. The combination of a retinoid, a phenolic agent and an antioxidant improves tolerance while retaining an optimal depigmenting action in reconstructed epidermis[J]. Dermatology, 2013, 227 (2) : 150-156. doi: 10.1159/000353578
[4]	于承仟, 徐学刚, 李远宏. 苯乙基间苯二酚的研究应用进展[J]. 中国皮肤性病学杂志, 2020, 34 (6) : 692-695.
[5]	Amnuaikit T, Limsuwan T, Khongkow P, et al. Vesicular carriers containing phenylethyl resorcinol for topical delivery system; liposomes, transfersomes and invasomes[J]. Asian J Pharm Sci, 2018, 13 (5) : 472-484. doi: 10.1016/j.ajps.2018.02.004 pmid: 32104421
[6]	Köpke D, Muller R H, Pyo S M. Phenylethyl resorcinol smartlipids for skin brightening-increased loading & chemical stability[J]. Eur J Pharm Sci, 2019, 137: 104992. doi: 10.1016/j.ejps.2019.104992
[7]	Kim B S, Na Y G, Choi J H, et al. The improvement of skin whitening of phenylethyl resorcinol by nanostructured lipid carriers[J]. Nanomaterials (Basel), 2017, 7 (9) : 241: 1-13. doi: 10.3390/nano7010001
[8]	Demina P A, Bukreeva T V. Pickering emulsion stabilized by commercial titanium dioxide nanoparticles in the form of rutile and anatase[J]. Nanotechnologies in Russia, 2018, 13 (7/8) : 425-429. doi: 10.1134/S1995078018040043
[9]	Tan J S J, Wong S L Y, Chen Z. Preparation of Janus titanium dioxide particles via ultraviolet irradiation of Pickering emulsions[J]. Advanced Materials Interfaces, 2020, 1901961.
[10]	Bao Y, Zhang Y, Liu P, et al. Novel fabrication of stable Pickering emulsion and latex by hollow silica nanoparticles[J]. Journal of Colloid and Interface Science, 2019, 553: 83-90. doi: S0021-9797(19)30681-2 pmid: 31195217
[11]	Gálvez-Vergara A, Fresco-Cala B, Cárdenas S. Switchable Pickering emulsions stabilized by polystyrene-modified magnetic nanoparticles[J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2020, 606.
[12]	Li W, Suzuki T, Minami H. The interface adsorption behavior in a Pickering emulsion stabilized by cylindrical polystyrene particles[J]. J Colloid Interface Sci, 2019, 552: 230-235. doi: 10.1016/j.jcis.2019.05.058
[13]	Bouhoute M, Taarji N, de Oliveira Felipe L, et al. Microfibrillated cellulose from argania spinosa shells as sustainable solid particles for O/W Pickering emulsions[J]. Carbohydr Polym, 2021, 251: 116990. doi: 10.1016/j.carbpol.2020.116990
[14]	Shi A, Feng X, Wang Q, et al. Pickering and high internal phase Pickering emulsions stabilized by protein-based particles: A review of synthesis, application and prospective[J]. Food Hydrocolloids, 2020, 109: 106117. doi: 10.1016/j.foodhyd.2020.106117
[15]	Zhu F. Starch based Pickering emulsions: Fabrication, properties, and applications[J]. Trends in Food Science & Technology, 2019, 85: 129-137.
[16]	Zhai X, Lin D, Liu D, et al. Emulsions stabilized by nanofibers from bacterial cellulose: New potential food-grade Pickering emulsions[J]. Food Res Int, 2018, 103: 12-20. doi: S0963-9969(17)30711-1 pmid: 29389597
[17]	Tiong A C Y, Tan I S, Foo H C Y, et al. Macroalgae-derived regenerated cellulose in the stabilization of oil-in-water Pickering emulsions[J]. Carbohydr Polym, 2020, 249: 116875. doi: 10.1016/j.carbpol.2020.116875
[18]	Jia Y, Zheng M, Xu Q, et al. Rheological behaviors of Pickering emulsions stabilized by tempo-oxidized bacterial cellulose[J]. Carbohydr Polym, 2019, 215: 263-271. doi: 10.1016/j.carbpol.2019.03.073
[19]	Ahmed R, Wang M, Qi Z, et al. Pickering emulsions based on the pH-responsive assembly of food-grade chitosan[J]. ACS Omega, 2021, 6 (28) : 17915-17922. doi: 10.1021/acsomega.1c01490 pmid: 34308026
[20]	Terescenco D, Hucher N, Picard C, et al. Sensory perception of textural properties of cosmetic Pickering emulsions[J]. Int J Cosmet Sci, 2020, 42 (2) : 198-207. doi: 10.1111/ics.v42.2
[21]	Almajidi Y Q, Gupta J, Sheri F S, et al. Advances in chitosan-based hydrogels for pharmaceutical and biomedical applications: A comprehensive review[J]. Int J Biol Macromol, 2023, 253 (6) : 127278.
[22]	Meng W, Sun H, Mu T, et al. Chitosan-based Pickering emulsion: A comprehensive review on their stabilizers, bioavailability, applications and regulations[J]. Carbohydr Polym, 2023, 304: 120491. doi: 10.1016/j.carbpol.2022.120491
[23]	Bhutto R A, Wang M, Iqbal S, et al. Curcumin-loaded Pickering emulsion stabilized by pH-induced self-aggregated chitosan particles: Effects of degree of deacetylation and molecular weight[J]. Food Hydrocolloids, 2024, 147: 109422. doi: 10.1016/j.foodhyd.2023.109422
[24]	Saiki P, Mello-Andrade F, Gomes T, et al. Sediment toxicity assessment using zebrafish (Danio rerio) as a model system: Historical review, research gaps and trends[J]. Sci Total Environ, 2021, 793: 148633. doi: 10.1016/j.scitotenv.2021.148633
[25]	董思颖, 陈亮, 张璐, 等. 基于斑马鱼实验模型评价人参玫瑰膏改善气血健康作用[J]. 时珍国医国药, 2021, 32 (9) : 2295-2298.
[26]	Bai L, Huan S, Xiang W, et al. Pickering emulsions by combining cellulose nanofibrils and nanocrystals: Phase behavior and depletion stabilization[J]. Green Chemistry, 2018, 20 (7) : 1571-1582. doi: 10.1039/C8GC00134K
[27]	Ye F, Miao M, Cui S W, et al. Characterisations of oil-in-water Pickering emulsion stabilized hydrophobic phytoglycogen nanoparticles[J]. Food Hydrocolloids, 2018, 76: 78-87. doi: 10.1016/j.foodhyd.2017.05.003
[28]	Mession J L, Assifaoui A, Lafarge C, et al. Protein aggregation induced by phase separation in a pea proteins-sodium alginate-water ternary system[J]. Food Hydrocolloids, 2012, 28 (2) : 333-343. doi: 10.1016/j.foodhyd.2011.12.022
[29]	Argatov I, Iantchenko A, Kocherbitov V. How to define the storage and loss moduli for a rheologically nonlinear material? A constructive review of nonlinear rheological measures[J]. Continuum Mechanics and Thermodynamics, 2017, 29: 1375-1387. doi: 10.1007/s00161-017-0584-8
[30]	Costa A L R, Gomes A, Cunha R L. One-step ultrasound producing O/W emulsions stabilized by chitosan particles[J]. Food Research International, 2018, 107: 717-725. doi: S0963-9969(18)30150-9 pmid: 29580539
[31]	Ahmed Bhutto R, Hira Bhutto N U A, Wang M, et al. Curcumin-loaded Pickering emulsion stabilized by pH-induced self-aggregated chitosan particles: Effects of degree of deacetylation and molecular weight[J]. Food Hydrocolloids, 2024, 147: 109422. doi: 10.1016/j.foodhyd.2023.109422
[32]	Lv H, Wang Z, An J, et al. Preparation and emulsifying properties of carbon-based Pickering emulsifier[J]. Processes, 2023, 11: 1070. doi: 10.3390/pr11041070
[33]	Elmastas M, Turkekul I, Ozturk L, et al. Antioxidant activity of two wild edible mushrooms (Morchella vulgaris and Morchella esculanta) from north turkey[J]. Combinatorial Chemistry & High Throughput Screening, 2006, 9 (6) : 443-448.
[34]	Chen W, Pan H, Sheng Y, et al. Pickering emulsion stabilized by sugarcane leaf polyphenols-zein covalent nanoparticles for curcumin delivery: In-vitro and inhibition of oxidative hemolytic activity evaluation[J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2024, 687: 133539. doi: 10.1016/j.colsurfa.2024.133539
[35]	Woo K, Shih J, Fraser S E. Fate maps of the zebrafish embryo[J]. Curr Opin Genet Dev, 1995, 5 (4) : 439-443. pmid: 7580134
[36]	Brönnimann D, Annese T, Gorr T A, et al. Splitting of circulating red blood cells as an in vivo mechanism of erythrocyte maturation in developing zebrafish, chick and mouse embryos[J]. J Exp Biol, 2018, 221 (15) : jeb184564-jeb184576.
[37]	Jong J L, Zon L I. Use of the zebrafish system to study primitive and definitive hematopoiesis[J]. Annu Rev Genet, 2005, 39: 481-501. pmid: 16285869
[38]	Caro M, Iturria I, Martinez-Santos M, et al. Zebrafish dives into food research: Effectiveness assessment of bioactive compounds[J]. Food Funct, 2016, 7 (6) : 2615-2623. doi: 10.1039/c6fo00046k pmid: 27109696
[39]	Behar R Z, Luo W, Lin S C, et al. Distribution, quantification and toxicity of cinnamaldehyde in electronic cigarette refill fluids and aerosols[J]. Tob Control, 2016, 25 (S2) : ii94-ii102.
[40]	Stockert J C, Horobin R W, Colombo L L, et al. Tetrazolium salts and formazan products in cell biology: Viability assessment, fluorescence imaging, and labeling perspectives[J]. Acta Histochem, 2018, 120 (3) : 159-167. doi: S0065-1281(17)30474-9 pmid: 29496266
[41]	管淑玉. 黑色素相关研究中动物模型的应用进展[J]. 中国实验动物学报, 2009, 17 (6) : 475-477.
[42]	Zhang J, Chambers I, Yun S, et al. Hrg1 promotes heme-iron recycling during hemolysis in the zebrafish kidney[J]. PLoS Genet, 2018, 14 (9) : e1007665.
[43]	Logan D W, Burn S F, Jackson I J. Regulation of pigmentation in zebrafish melanophores[J]. Pigment Cell Res, 2006, 19 (3) : 206-213. doi: 10.1111/pcr.2006.19.issue-3

样品名称	DPPH乙醇溶液/mL	PR CS-PE乳液/mL	PE乳液/ mL	PR-TW80乳液/mL	TW80乳液/ mL	PR乙醇乳液/ mL	乙醇/ mL
DPPH乙醇溶液（A₀）	2	0	0	0	0	0	0
PR CS-PE乳液（A₁）	2	2	0	0	0	0	0
PR CS-PE乳液（A₂）	0	2	0	0	0	0	2
PE乳液（A₁）	2	0	2	0	0	0	0
PE乳液（A₂）	0	0	2	0	0	0	2
PR-TW80乳液（A₁）	2	0	0	2	0	0	0
PR-TW80乳液（A₂）	0	0	0	2	0	0	2
TW80乳液（A₁）	2	0	0	0	2	0	0
TW80乳液（A₂）	0	0	0	0	2	0	2
PR乙醇溶液（A₁）	2	0	0	0	0	2	0
PR乙醇溶液（A₂）	0	0	0	0	0	2	2

名称	PR CS-PE乳液/mL	CS-PE乳液/mL	PR乙醇溶液/mL	乙醇/mL	ABTS母液/mL
PR CS-PE乳液（A₁）	1	0	0	0	3
CS-PE乳液（A₁）	0	1	0	0	3
PR乙醇溶液（A₁）	0	0	1	0	3
乙醇（A₁）	0	0	0	1	3

Preparation and skin-whitening activity evaluation of phenylethyl resorcinol/chitosan Pickering emulsion

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