Abstract:

In this study, water-soluble ceramide NP liposomes （CNPL） were prepared using high-pressure homogeneous microfluidization technology, and the physical and chemical properties of CNPL were thoroughly investigated. The repair mechanism and efficacy of CNPL were assessed utilizing a UVB-injured 3D epidermal skin model, alongside an examination of the repair effects on physical injury through the caudal fin excision model in zebrafish embryos. The experimental results indicate that CNPL has high transparency and stability, and the transmittance of CNPL aqueous solution within 10.00% mass fraction is close to that of the water. The average particle size is 22.25 nm （PDI=0.241）, and the pH range is 6.70-6.90. The permeability increases 3.8 times after 12 h. The improvement rates of FLG and Claudin-1 in 3.00% volume fraction CNPL are 128.21% and 73.68%, respectively. While the improvement rates of FLG and Claudin-1 in 5.00% volume fraction CNPL are 194.87% and 100.00%, respectively. The higher the concentration of CNPL, the stronger the improvement effect of FLG and Claudin-1, with 0.01 g/L and 0.03 g/L CNPL promoting the repair of zebrafish embryonic caudal fin by 3.05% and 8.60%, respectively. Overall, the findings suggest that CNPL possesses excellent water solubility, stability, transmittance, as well as superior transdermal absorption efficiency and bioavailability characteristics. It demonstrates significant reparative effects against UVB-induced light damage as well as physical injuries, thereby aiding in skin barrier restoration, delaying photoaging processes, promoting wound healing efforts, and presenting itself as an advantageous component for broader applications within cosmetic formulations.

Key words: ceramide liposomes, repair, light injury, 3D epidermal skin model, caudal fin repair of zebrafish embryos