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China Surfactant Detergent & Cosmetics ›› 2016, Vol. 46 ›› Issue (7): 414-418.doi: 10.13218/j.cnki.csdc.2016.07.011

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Separation and purification of polyphenols from Ampelopsis grossedentata and its inhibition effect on oral mutating streptococci

JIANG Xu-dong1,YANG Wei-hao2,ZHOU Chuan-jian2,LU Kai-ling2,LIN Cui-wu3,4   

  1. 1.School of Medicine,Guangxi University of Science and Technology,Liuzhou,Guangxi 545005,China;
    2.Liuzhou LMZ Co.,Ltd.,Liuzhou,Guangxi 545006,China;
    3.School of Chemistry and Chemical Engineering, Guangxi University,Nanning,Guangxi 530004,China;
    4.Guangxi Colleges and Universities Key Laboratory of Applied Chemistry Technology and Resource Development,Nanning,Guangxi 530004,China
  • Online:2016-07-22 Published:2019-04-18

Abstract: Via the determination of polyphenol mass concentration by Folin-Ciocaileu colorimetry,calculation of the adsorption and elution rate,as well as comparison of effect of five kinds of macroporous resin on static adsorption and desorption rates of polyphenols from Ampelopsis grossedentata,HPD-100 resin was selected to conduct the optimization of purification technology by single factor tests.Results showed that the optimum purification conditions are:extract of polyphenol mass concentration of feed sample,2.4 g/L of 6.5 BV,flow rate,2.0 BV/h;elution with 7.5 BV of 60% ethanol at a flow rate of 3 BV/h.The mass fraction of polyphenols can be enhanced from 29.9% to 69.8%.The results showed that the inhibition activity of polyphenols from Ampelopsis grossedentata on growth of oral mutating streptococci is very obvious and the MIC value achieves 3.13 g/L.

Key words: oral care, polyphenols from Ampelopsis grossedentata, purification, mutating streptococci

CLC Number: 

  • TQ658.4+9