壮药菲牛蛭提取物的美白作用及其机制初探

doi:10.3969/j.issn.1001-1803.2022.01.006

Abstract

Abstract:

This research was aimed to study the whitening effect and mechanism of Zhuang medicine Poecilobdella manillensis extract. MTT method was used to detect the inhibitory effect of Poecilobdella manillensis extract on the proliferation of melanoma cells （B16）. Spectrophotometry was used to detect the effect of Poecilobdella manillensis extract on TYR activity and melanin synthesis in B16 cells, and the scavenging ability of DPPH, ·OH and ·O₂^- free radicals and the total antioxidant capacity were also detected. Based on an ultraviolet-irradiated nude mice skin pigmentation model, Masson Fontana silver staining was used to show the distribution of melanin in skin, and the levels of TYR, SOD, MDA, α-MSH, bFGF, NGF, IL-1α, TNF-α and IFN-γ in serum were also measured. The results show that the IC₅₀ of B16 cells proliferation are 230.66, 109.70, 53.18 μg/mL after Poecilobdella manillensis extract treatment at 24, 48 and 72 h, respectively. The IC₅₀ of arbutin at 24, 48 and 72 h are 1.11, 1.40 and 1.30 times higher than those of Poecilobdella manillensis extract, respectively. The non-cytotoxic concentration of Poecilobdella manillensis extract could significantly increase the tyrosinase activity and melanin content of B16 cells. The Poecilobdella manillensis extract has strong antioxidant ability in vitro. The IC₅₀ of DPPH, ·OH and ·O₂^- free radicals are 0.37, 0.38 and 0.43 g/L, respectively. The total antioxidant capacity of 0.8 g/L Poecilobdella manillensis extract is equivalent to 1.0 g/L vitamin E. Poecilobdella manillensis extract could significantly reduce the number of melanocytes in skin of nude mice, increase the content of SOD in serum, decrease the contents of MDA, α-MSH, bFGF, NGF, IL-1α, TNF-α and IFN-γ and TYR activity. Zhuang medicine Poecilobdella manillensis extract has a good whitening effect, and its mechanism may be related to the inhibition of tyrosinase activity and antioxidation capacity.

Key words: Poecilobdella manillensis, whitening effect, melanoma cells, tyrosinase, antioxidant

CLC Number:

TQ658

He Ruiyuan,Wang Shuo,Wei Guili,Gong Xiaomei,Wu Peiying,Miao Jianhua. Study of whitening effect and the mechanism of Zhuang medicine Poecilobdella manillensis extract[J].China Surfactant Detergent & Cosmetics, 2022, 52(1): 35-43.

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References 21

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	质量浓度/（μg/mL）	抑制率/%				质量浓度/（μg/mL）	抑制率/%
	质量浓度/（μg/mL）	24 h	48 h	72 h		质量浓度/（μg/mL）	24 h	48 h	72 h
菲牛蛭提取物	10	1.16±0.45	5.46±0.23	13.86±0.75	熊果苷	10	1.89±1.03	7.21±1.68	10.71±0.05
	20	3.01±0.59	13.49±3.37	21.41±2.15		20	3.96±1.84	10.53±0.47	20.56±0.32
	40	15.96±3.48	30.87±3.03	44.99±1.68		40	12.23±2.81	23.15±0.46	40.73±2.12
	60	16.75±2.56	34.17±4.46	48.32±2.14		60	14.76±2.52	29.28±2.22	46.81±3.87
	80	17.34±2.17	38.72±2.78	55.82±1.38		80	18.88±1.12	30.60±2.05	48.88±3.12
	100	26.35±4.69	47.02±5.67	76.37±7.35		100	25.06±4.28	42.37±5.17	61.25±4.06
	IC₅₀	230.66	109.70	53.18		IC₅₀	255.40	153.19	68.96

	质量浓度/（μg/mL）	48 h抑制率/%		质量浓度/（μg/mL）	48 h抑制率/%
熊果苷	14	10.96±1.25	菲牛蛭提取物	14	21.63±1.44^△△
	7	7.13±0.94		7	11.19±1.11^△△
	3.5	5.64±0.54		3.5	8.24±1.20^△

	质量浓度/（μg/mL）	48 h抑制率/%		质量浓度/（μg/mL）	48 h抑制率/%
熊果苷	14	7.12±1.03	菲牛蛭提取物	14	9.90±0.93^△
	7	5.81±1.09		7	8.03±0.21^△
	3.5	4.61±0.36		3.5	4.98±0.39

	质量浓度/（g/L）	清除率/%				质量浓度/（g/L）	清除率/%
	质量浓度/（g/L）	DPPH	·OH	·O₂^-		质量浓度/（g/L）	DPPH	·OH	·O₂^-
菲牛蛭提取物	0.1	21.23±1.73	20.23±2.55	23.43±3.21	V_E	0.1	15.36±1.54	15.37±1.95	20.35±2.31
	0.2	30.32±1.44	30.78±2.22	35.2±2.13		0.2	21.05±2.57	27.69±2.38	25.67±1.58
	0.3	38.16±2.31	38.16±1.89	40.53±1.56		0.3	28.16±2.17	31.44±1.96	31.28±3.25
	0.4	46.76±1.42	44.58±2.07	48.78±1.77		0.4	36.55±3.53	39.08±2.39	36.25±3.50
	0.5	56.32±2.09	50.32±1.07	56.32±2.33		0.5	43.67±2.13	44.27±1.85	41.98±2.05
	0.6	67.19±2.19	63.73±1.87	66.77±1.99		0.6	50.37±3.45	56.38±2.37	47.38±2.66
	0.7	73.66±1.89	70.67±1.53	73.87±2.07		0.7	55.69±2.67	62.34±2.22	52.87±1.89
	0.8	75.39±2.33	72.72±2.07	75.56±1.53		0.8	68.25±3.41	66.02±3.57	59.13±2.43
	0.9	78.13±1.17	73.32±1.02	76.43±1.63		0.9	74.01±2.57	70.08±2.59	68.24±2.33
	1.0	80.12±1.87	78.21±1.89	77.25±2.78		1.0	76.25±3.74	70.39±2.58	69.52±1.66

	质量浓度/（g/L）	总抗氧化能力/mL		质量浓度/（g/L）	总抗氧化能力/mL
菲牛蛭提取物	0.1	1.25±0.31	V_E	0.1	0.58±0.38
	0.2	2.66±0.61		0.2	0.95±0.15
	0.3	3.18±0.58		0.3	1.28±0.34
	0.4	3.96±0.62		0.4	1.89±0.21
	0.5	4.51±0.45		0.5	2.38±0.47
	0.6	5.27±0.42		0.6	3.66±0.48
	0.7	5.83±0.47		0.7	4.58±0.34
	0.8	6.33±0.58		0.8	5.13±0.61
	0.9	6.99±0.61		0.9	5.97±0.32
	1.0	7.15±0.56		1.0	6.52±0.57

Study of whitening effect and the mechanism of Zhuang medicine Poecilobdella manillensis extract

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组别	给药质量浓度/（g/L）	MDA/（nmol/mL）	SOD/（nmol/mL）	TYR/（nmol/mL）
空白组	—	9.54±0.98	127.76±9.48	77.86±2.25
模型组	—	14.95±1.59^△	106.78±6.05^△	90.36±1.69^△
阳性组	0.100	10.38±1.39^**	120.92±10.10^**	80.12±2.31^**
低剂量组	0.025	14.70±0.59	105.35±8.98	87.22±2.09
中剂量组	0.050	12.79±1.57	111.55±5.87	81.09±3.31^**
高剂量组	0.100	9.89±1.13^**	122.98±8.26^**	79.87±1.49^**

组别	给药质量浓度/（g/L）	α-MSH/（nmol/mL）	bFGF/（nmol/mL）	NGF/（nmol/mL）
空白组	—	25.63±3.69	58.39±8.21	103.62±9.12
模型组	—	39.65±6.39^△△	89.36±7.01^△△	134.25±6.20^△△
阳性组	0.100	16.99±3.25^**	61.37±5.23^**	90.32±8.65^**
低剂量组	0.025	36.31±6.92	88.24±7.25	128.36±6.38
中剂量组	0.050	28.36±5.03^*	82.33±7.20	113.25±5.22^*
高剂量组	0.100	20.50±3.27^**	68.61±5.07^**	100.58±6.27^**

组别	给药质量浓度/（g/L）	IL-1α/（nmol/mL）	TNF-α/（nmol/mL）	IFN-γ/（nmol/mL）
空白组	—	38.88±5.37	22.22±5.48	160.62±10.85
模型组	—	79.65±5.21^△△	47.66±6.31^△△	199.45±10.65^△△
阳性组	0.100	41.31±3.63^**	27.11±3.15^**	137.61±12.15^**
低剂量组	0.025	74.32±6.17	43.39±5.92	189.24±11.93
中剂量组	0.050	70.08±8.67^*	38.71±6.71^*	176.46±10.26^**
高剂量组	0.100	45.40±3.90^**	26.86±3.29^**	152.38±11.93^**

组别	给药质量浓度/（g/L）	阳性面积比例（×100）	MOD（×100）	IOD（×100）
空白组	—	0.05±0.03	10.32±3.42	0.13±0.10
模型组	—	0.80±0.34^△	14.03±5.61^△	0.25±0.04^△
阳性组	0.100	0.65±0.24^*	12.09±4.37^*	0.20±0.09
低剂量组	0.025	0.78±0.31	13.99±7.52	0.23±0.8
中剂量组	0.050	0.66±0.12	13.78±2.61	0.18±0.05
高剂量组	0.100	0.30±0.23^*	11.02±6.32^*	0.15±0.04^*

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