天麻素对UVB损伤皮肤的修复作用

doi:10.3969/j.issn.2097-2806.2025.06.012

Abstract

Abstract:

In order to investigate the reparative effect of gastrodin （GAS） in the skin damaged by medium-wave ultraviolet （UVB） rays, human keratinocytes （HaCaT） was used to build UVB radiation damage cell model. The inhibitory effect of GAS on the secretion of TNF-α and PGE₂ in UVB-irradiated cells, and the modulation of the mRNA expression levels of AQP3 and FLG, as well as the effects on the content of the two proteins, were investigated by enzyme-linked immunosorbent assay （ELISA）, real-time quantitative fluorescence PCR （qRT-PCR）, and immunofluorescent assays. The results show that GAS solution at a mass concentration of 200 µg/mL can repair the inflammatory damage produced by UVB, inhibit the secretion of TNF-α and PGE2 （P<0.05 and P<0.001）, up-regulate the mRNA expression levels of FLG and AQP3 （P<0.01 and P<0.05）, and increase the protein content of FLG and AQP3 （P<0.01 and P<0.001）. It elevates the skin hydration and promotes the repair of the skin barrier function, thus reinforcing the barrier structure, which can provide scientific experimental basis for the application of GAS in restorative cosmetics.

Key words: gastrodin, UVB, HaCaT, skin repair

CLC Number:

TQ658

Haodong Li, Xingyi Wu, Ying Zhang, Weilian Wang, Wei Wang, Li Li. Repairing effect of gastrodin on UVB-damaged skin[J].China Surfactant Detergent & Cosmetics, 2025, 55(6): 779-784.

Figures/Tables 13

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References 12

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基因名称	引物方向	引物序列
GAPDH	F	GGAGCGAGATCCCTCCAAAAT
GAPDH	R	GGCTGTTGTCATACTTCTCATGG
AQP3	F	CCGTGACCTTTGCCATGTG
AQP3	R	CGAAGTGCCAGATTGCATCATAA
FLG	F	GGACAGGAACAATCATCGGGG
FLG	R	CAACCTCTCGGAGTCGTCTG

Repairing effect of gastrodin on UVB-damaged skin

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