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日用化学工业（中英文）

日用化学工业 ›› 2022, Vol. 52 ›› Issue (3): 309-315.doi: 10.3969/j.issn.1001-1803.2022.03.012

• 专论与综述 • 上一篇    下一篇

微生物快速计数方法及其在化妆品检验中的应用分析

刘丰,王晓冲,罗俊,王晓炜(),应国红()   

  1. 深圳市药品检验研究院 国家药品监督管理局化妆品监测评价重点实验室,广东 深圳 518057
  • 收稿日期:2021-05-25 修回日期:2022-02-25 出版日期:2022-03-22 发布日期:2022-03-21
  • 通讯作者: 王晓炜,应国红

Application analysis of rapid microbial counting method in the detection of aerobic bacterial count in cosmetics

Liu Feng,Wang Xiaochong,Luo Jun,Wang Xiaowei(),Ying Guohong()   

  1. NMPA Key Laboratory for Monitoring and Evaluation of Cosmetics, Shenzhen Institute for Drug Control, Shenzhen, Guangdong 518057, China
  • Received:2021-05-25 Revised:2022-02-25 Online:2022-03-22 Published:2022-03-21
  • Contact: Xiaowei Wang,Guohong Ying

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摘要：

国内外的化妆品菌落总数检验标准方法基本一致,采用的是经典的平板计数法,该方法操作简单,但完成微生物检验过程所需的时间较长。随着微生物学技术的发展,逐渐开发出了基于各种原理的微生物快速计数方法。通过对比平板计数法和各种微生物快速计数方法在微生物检测中的优缺点,综合考虑检验时间、检验准确性以及微生物快速计数方法在化妆品微生物检验中的可行性,得出荧光显微镜计数法和流式细胞仪计数法在化妆品菌落总数检验中具有潜在的应用价值。荧光显微镜计数法和流式细胞仪计数法通过直接计数样品中的微生物数量,检验时间较短;不需要进行微生物培养,无需考虑化妆品中的防腐剂对微生物生长的抑制性,检验准确性较高。将微生物快速计数方法纳入化妆品菌落总数检测标准体系中,作为现行标准的补充,在化妆品菌落总数检验中具有重要的意义。

关键词: 化妆品, 菌落总数, 平板计数法, 微生物快速计数方法

Abstract:

The plate count method has been used as a classical method at home and abroad for the aerobic bacterial count in cosmetics, which is simple to operate, but it takes a long time to complete the entire detection process. With the development of microbiological technology, a variety of rapid microbial counting methods based on various principles have been gradually developed. By comparing the advantages and disadvantages of the plate count methods and various rapid microbial counting methods for the aerobic bacterial count in cosmetics, the length of detection time, the accuracy of the aerobic bacterial count, and the feasibility of rapid microbial counting method in the detection of aerobic bacterial count in cosmetics were comprehensively considered. It can be concluded that the fluorescence microscopy counting method and flow cytometry counting method have potential in the detection of aerobic bacterial count in cosmetics. The reasons are as follows, TEMPO® counting method, molecular biology counting method, ELISA （enzyme-linked immunosorbent assay） counting method, optical biosensors counting method, and mass spectrometry counting method have high specificity for bacteria and are only suitable for quantification of certain microorganisms, while microbial metabolism counting method, microfluidics counting methods, MicroSnapTM counting method, and SimplateTM counting method require microbial culture, thus preservatives in cosmetics will affect the accuracy of the total number of microorganisms in the sample. Although the fluorescence intensity counting method is suitable for the aerobic bacterial count in cosmetics, its sensitivity is quite low. The fluorescence microscopy counting method and flow cytometry counting method are suitable for the aerobic bacterial count in cosmetics for two reasons. The first reason is that the fluorescence microscopy counting method and flow cytometry counting method directly count the number of microorganisms in the sample so that the detection time is shorter. The second reason is that there is no need for microbial culture. Therefore, there is no need to consider the inhibitory effect of preservatives in cosmetics on microbial growth, so that the detection accuracy is higher. The introduction of rapid microbial counting methods into the standard system of the aerobic bacterial count in cosmetics, as a supplement to the current standard, is of great significance for aerobic bacterial count in cosmetics.

Key words: cosmetics, aerobic bacterial count, plate count method, rapid microbial counting method

中图分类号: 

  • TQ658