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日用化学工业（中英文）

日用化学工业（中英文） ›› 2026, Vol. 56 ›› Issue (9): 1214-1221.doi: 10.3969/j.issn.2097-2806.2026.09.010

• 开发与应用 • 上一篇    下一篇

肌肽、绿原酸和姜黄素在HFF-1糖化损伤细胞模型中的功效作用研究

加裕盈,曹晨灿,刘蕾*()   

  1. 北京工商大学 轻工科学与工程学院 北京市植物资源研究开发重点实验室， 北京 102488
  • 收稿日期:2026-01-06 修回日期:2026-08-18 出版日期:2026-09-22 发布日期:2026-09-30

Study on the efficacy of carnosine, chlorogenic acid, and curcumin in an HFF-1 glycation-injured cell model

Yuying Jia,Chencan Cao,Lei Liu*()   

  1. Beijing Key Laboratory of Plant Resources Research and Development, School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 102488, China
  • Received:2026-01-06 Revised:2026-08-18 Online:2026-09-22 Published:2026-09-30
  • Contact: *E-mail: liulei@btbu.edu.cn.

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摘要：

为了探究三种原料基于人包皮成纤维细胞（HFF-1）糖化损伤细胞模型的抗糖化作用，并验证模型的有效性。根据文献调研选用已知具有抗糖化作用且作用途径不同的肌肽（Car）、绿原酸（CGA）、姜黄素（Cur）三种原料，将其应用到阶段性糖化损伤模型中。细胞模型分为三个阶段，分别由葡萄糖（Glu）溶液、乙二醛（GO）溶液和晚期糖基化终产物（AGEs）溶液刺激HFF-1构建。利用荧光探针法测定胞内活性氧（ROS）平均荧光强度，利用ELISA法测定胞内羧甲基赖氨酸（CML）含量和细胞上清液弹性蛋白（ELN）含量。结果显示，姜黄素的胞内自由基清除能力最佳，其次为绿原酸、肌肽。对CML生成的抑制能力在早期模型中绿原酸最佳，其次为肌肽、姜黄素；在中、晚期模型中姜黄素最佳，其次为肌肽、绿原酸。本研究进一步得到具有创新性的阶段性差异结论：绿原酸对细胞内CML的调控优势主要集中于糖化早期与末期，肌肽主要集中于中晚期。对于细胞上清液中ELN的保护作用肌肽效果最佳，绿原酸与姜黄素效果相近。肌肽、绿原酸、姜黄素的抗糖化作用具有阶段性差异，在评价效果时要考虑阶段性综合考量。此外验证了三种阶段性糖化损伤模型可有效检验原料功效，为后续功效评价提供一定的参考价值。

关键词: 糖化损伤, 抗糖化, 体外细胞模型, 功效作用

Abstract:

In this study, three cosmetic ingredients with reported anti-glycation activity—carnosine, chlorogenic acid, and curcumin—were evaluated across glycation models representing different stages of glycation-related damage. Three stage-specific glycation injury models were established in HFF-1 human dermal fibroblasts via stimulation with a glucose solution （early stage）, a glyoxal solution （middle stage）, and an advanced glycation end products （AGEs） solution （late stage）, respectively. Intracellular reactive oxygen species （ROS） levels were quantified using a fluorescent probe assay by measuring mean fluorescence intensity, while intracellular Nε-carboxymethyllysine （CML） content and elastin （ELN） levels in the cell culture supernatant were determined by ELISA. The results show that, in terms of intracellular free-radical scavenging capacity, curcumin exhibits the highest potency, followed by chlorogenic acid and carnosine. With respect to the inhibition of CML formation, chlorogenic acid is most effective in the early-stage model, followed by carnosine and curcumin; in the middle- and late-stage models, curcumin shows the strongest inhibitory effect, followed by carnosine and chlorogenic acid. Regarding the protection of ELN in the culture supernatant, carnosine exhibits the greatest protective effect, while chlorogenic acid and curcumin showed similar effect. Overall, these findings indicate that the anti-glycation effects of carnosine, chlorogenic acid, and curcumin differ in a stage-dependent manner. In addition, the three stage-specific glycation damage models effectively discriminate the efficacy of the tested ingredients, thereby providing a useful reference for subsequent efficacy evaluations.

Key words: glycation damage, anti-glycation, in vitro cell model, efficacy

中图分类号: 

  • TQ658