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日用化学工业(中英文) ›› 2025, Vol. 55 ›› Issue (5): 668-676.doi: 10.3969/j.issn.2097-2806.2025.05.016

• 分析与检测 • 上一篇    

化妆品微生物ATP生物荧光增幅检测技术的双培养体系构建及可行性研究

李婷1,马紫英1,刘吉泉2,崔生辉3,景宇1,白飞荣1,姚粟1,*()   

  1. 1.中国食品发酵工业研究院有限公司,北京 100015
    2.Procter & Gamble International Operations,新加坡 138547
    3.中国食品药品检定研究院,北京 100050
  • 收稿日期:2024-05-11 修回日期:2025-04-30 出版日期:2025-05-22 发布日期:2025-06-11
  • 基金资助:
    化妆品微生物ATP生物荧光增幅24 h检测方法建立及验证研究

Establishment and feasibility study of a dual enrichment system for amplified ATP bioluminescence microbial detection assay in cosmetics

Ting Li1,Ziying Ma1,Jiquan Liu2,Shenghui Cui3,Yu Jing1,Feirong Bai1,Su Yao1,*()   

  1. 1. China National Research Institute of Food & Fermentation Industries Co., Ltd., Beijing 100015, China
    2. Procter & Gamble International Operations, Singapore 138547, Singapore
    3. National Institutes for Food and Drug Control, Beijing 100050, China
  • Received:2024-05-11 Revised:2025-04-30 Online:2025-05-22 Published:2025-06-11
  • Contact: *E-mail: Su.yao@china-cicc.org.

摘要:

建立了基于双培养体系的化妆品微生物ATP生物荧光增幅24 h检测方法,开展了富集培养体系促生长能力测试和产品适用性试验。选择11株生长缓慢的丝状真菌、酵母菌和细菌代表菌株,针对培养温度、增菌培养基配方等参数进行优化,开发了促进真菌生长的培养体系为TLE培养基,培养温度25 ℃;细菌培养体系为MTAT培养基、培养温度32.5 ℃,建立了ATP生物荧光增幅24 h检测方法。收集的32株与化妆品原料及生产环境相关的菌株在富集培养体系中生长良好,ATP生物荧光增幅24 h检测方法检测结果均为阳性,符合促生长试验要求。分别选取了不同功能宣称、使用部位、生产剂型、使用方法的6种化妆品,接种6株《中国药典》(2020版)推荐菌株,进行了产品适用性试验。结果表明,供试产品背景值符合方法要求,接菌后的供试样品检测结果均为阳性。因此,ATP生物荧光增幅24 h检测方法在化妆品及相关领域开展微生物快速筛选具有良好应用前景。

关键词: ATP生物荧光增幅, 化妆品, 双培养体系, 促生长能力, 产品适用性

Abstract:

To improve the microbial detection efficiency in cosmetics, an amplified ATP bioluminescence 24 h assay with dual enrichment system was established. Meanwhile, growth promotion test of an enrichment medium and product suitability test was carried out. 11 of slow-growing fungi, yeasts and bacteria were selected as representative strains, and the key parameters such as culture temperature and enrichment medium formula were optimized. The results show that the enrichment system that promoted fungal growth is TLE medium incubated at 25 ℃, and the bacterial enrichment system is MTAT medium incubated at 32.5 ℃. 32 representative strains relevant to raw materials and production environment of cosmetics are collected and tested. They all grow well in the enrichment system,and the results of amplified ATP bioluminescence 24 h assay are all positive. Subsequently, six kinds of cosmetics with different functional claims, use parts, production dosage forms and use methods are selected and inoculated with six reference strains recommended by Chinese Pharmacopoeia (2020) for product suitability test. The background values of the tested cosmetics meet the requirements of the detection method. Results of spiking test show that all the samples tested are positive. Therefore, amplified ATP bioluminescence 24 h assay has a promising application prospect in the rapid screening of microorganisms in cosmetic fields.

Key words: amplified ATP bioluminescence, cosmetics, dual enrichment system, growth promotion test, product suitability test

中图分类号: 

  • TQ658