植物复合舒缓剂（CAP）对外源性皮肤刺激的保护作用

doi:10.3969/j.issn.2097-2806.2023.07.004

摘要/Abstract

摘要：

作为抵御外部环境的第一道防线，皮肤不仅是一种物理屏障，而且是一种具有多种免疫细胞的免疫器官。这些免疫细胞在不同的皮肤层中组织结构良好，并与非免疫结构细胞（如角质形成细胞）建立通信网络，参与先天和适应性免疫反应，以确保皮肤免疫稳态。通过探讨皮肤免疫反应的起始感知、初始传递、初级效应阶段下不同类型细胞所产生的关键调节剂来研究植物复合舒缓剂（CAP）对外源性皮肤刺激的潜在保护作用及机制。同时，利用三维（3D）重建的人类表皮模型（EpiKutis）来模拟弱屏障下的皮肤免疫反应研究CAP在组织层面的作用。结果显示，在外源性刺激时，关键免疫调节因子如胸腺基质淋巴细胞生成素（TSLP）、白细胞介素-8（IL-8）、前列腺素E2（PGE-2）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）在角质形成细胞、3D EpiKutis模型、THP-1衍生细胞和肥大细胞中含量均显著升高，而CAP同步处理下可显著降低这些因子的表达量。此外，CAP剂量依赖性地改善由Poly（I:C）和LPS诱导导致的丝聚蛋白降低。结果表明，CAP通过促进皮肤屏障修复，调控皮肤免疫反应的起始、传递和效应阶段发挥重要作用。

关键词: 植物复合舒缓剂, 胸腺基质淋巴细胞生成素, 皮肤刺激性反应, 角质形成细胞, 单核细胞, 肥大细胞

Abstract:

The skin contains a myriad of immune cells and acts as the first line of defense system against the external environment both as a physical and immune barrier. The cutaneous immune system begins to work even before birth and continuously evolves throughout the lifetime to achieve optimal function by adapting to environmental cues. This study explored the potential mechanism of multi-herb extract CAP against exogenous cutaneous stimulation and discussed the key modulators that drove productive immune responses in skin epidermis and dermal layer immune cells, including keratinocytes, monocytes, and mast cells. Also, a three-dimensional （3D） reconstructed human epidermis model （EpiKutis） was used to perform relevant assays. CAP reduced the expressions of thymic stromal lymphopoietin （TSLP）, interleukin-8 （IL-8）, prostaglandin E2 （PGE-2）, interleukin-6 （IL-6）, and tumor necrosis factor-alpha （TNF-α） that were increased by exogenous stimuli in keratinocytes, 3D EpiKutis, THP-1- derived cells or mast cells. Furthermore, CAP dose-dependently promoted filaggrin level which was decreased by poly （I:C） and LPS induction. These results suggest that CAP might have regulatory functions on keratinocytes, monocytes, and mast cells, which play important roles on the sense, initial and primary stages of the skin inflammatory reactions, respectively.

Key words: multi-herb extract CAP, thymic stromal lymphopoietin, skin inflammatory reactions, keratinocytes, monocytes, mast cells

中图分类号:

TQ658

周利丹, 卢伊娜, 杨继峰, 施雪梅, 熊玥, 张磊. 植物复合舒缓剂（CAP）对外源性皮肤刺激的保护作用[J]. 日用化学工业（中英文）, 2023, 53(7): 757-764.

Zhou Lidan, Lu Yina, Yang Jifeng, Shi Xuemei, Xiong Yue, Zhang Lei. The protection mechanism of multi-herb extract CAP against exogenous cutaneous stimulation[J]. China Surfactant Detergent & Cosmetics, 2023, 53(7): 757-764.

图/表 4

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