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日用化学工业 ›› 2022, Vol. 52 ›› Issue (8): 839-843.doi: 10.3969/j.issn.1001-1803.2022.08.007

• 开发与应用 • 上一篇    下一篇

含发酵产物类化妆品微生物检查方法适用性研究

刘丰,罗俊,王晓炜*(),应国红*()   

  1. 深圳市药品检验研究院 国家药品监督管理局化妆品监测评价重点实验室,广东 深圳 518057
  • 收稿日期:2021-11-18 修回日期:2022-08-03 出版日期:2022-08-22 发布日期:2022-08-24
  • 通讯作者: 王晓炜,应国红

Discussion on the applicability of microbiological test method for cosmetics containing fermented ingredients

Liu Feng,Luo Jun,Wang Xiaowei*(),Ying Guohong*()   

  1. NMPA Key Laboratory for Monitoring and Evaluation of Cosmetics, Shenzhen Institute for Drug Control, Shenzhen, Guangdong 518057, China
  • Received:2021-11-18 Revised:2022-08-03 Online:2022-08-22 Published:2022-08-24
  • Contact: Xiaowei Wang,Guohong Ying

摘要:

为了考察含发酵产物类化妆品的卫生质量及探讨其防腐体系对微生物检查方法的影响,按照《化妆品安全技术规范》(2015年版)对30批含发酵产物类化妆品进行微生物检查,同时参考《中国药典》(2020年版)控制菌方法适用性及微生物计数方法适用性试验,对含发酵产物类化妆品进行微生物检查方法适用性研究。结果表明,含发酵产物类化妆品的卫生质量较好,合格率达到了96.67%。30批含发酵产物类化妆品中,有3批化妆品需采用供试液稀释法才能通过控制菌方法适用性试验;有4批化妆品的抑菌性非常强,即使采用1:100供试液,试验菌的回收率依然<0.5,需采用薄膜过滤法才能通过微生物计数方法适用性试验。30批含发酵产物类化妆品中共有5批化妆品具有较强的抑菌性,可能会影响化妆品微生物检验结果的准确性,因此很有必要在化妆品微生物检验之前进行微生物方法适用性试验。

关键词: 含发酵产物类化妆品, 微生物检验, 适用性, 抑菌性

Abstract:

For surveillance of the cosmetic hygiene for cosmetics containing fermented ingredients, 30 batches of cosmetics containing fermented ingredients were subjected to microbiological tests according to the Safety and Technical Standards for Cosmetics (2015 edition). Additionally, to investigate the influence of the antibacterial system therein on the applicability of bacteria control method and the applicability of microbial counting method, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Candida albicans and Aspergillus niger were used for test according to Chinese Pharmacopoeia (2020 edition). The results showed that, the hygienic quality of cosmetics containing fermented ingredients was good, with a pass rate of 96.67%. Among the 30 batches, the positive control of Escherichia coli did not grow in two batches of cosmetics, and the Staphylococcus aureus did not grow in one batch of cosmetics, and Pseudomonas aeruginosa grew normally in all cosmetics. A total of three batches did not pass the applicability of the control bacteria inspection method, and test-solution dilution method was needed to meet the requirements. There were four batches that had very strong antibacterial activity toward Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans, i.e., even using 1:100 test solution, the recovery rate of the test bacteria was still less than 0.5, which required the use of membrane filtration method to make the recovery rate of test bacteria reach 0.5-2.0. Among the 30 batches of cosmetics containing fermented ingredients, 5 batches of cosmetics had different degrees of antibacterial activity, which might affect the accuracy of microbial testing results. Currently, commercially available cosmetics containing fermented ingredients are added with chemical preservatives. However, probiotic fermentation ingredients are not only functional ingredients but also good biological preservatives. Thus, cosmetics containing fermented ingredients may seriously affect the detection rate of microorganisms therein. Therefore, it is necessary to conduct a suitability test before microbiological test for such cosmetics.

Key words: cosmetics containing fermented ingredients, microbiological test, applicability, microbial inhibition

中图分类号: 

  • TQ658