欢迎访问《日用化学工业(中英文)》,今天是

日用化学工业 ›› 2022, Vol. 52 ›› Issue (4): 383-389.doi: 10.3969/j.issn.1001-1803.2022.04.006

• 开发与应用 • 上一篇    下一篇

表皮葡萄球菌发酵提取物对人体皮肤屏障的影响

帖航1,4,吕瑜峰2,张阳3,徐亮4,*(),闫妍1,*()   

  1. 1. 中国检验检疫科学研究院,北京 100176
    2. 天津商业大学,天津 300000
    3. 上海时垠电子商务有限公司,上海 200000
    4. 天津医科大学 药学院,天津 300070
  • 收稿日期:2021-06-21 修回日期:2021-03-23 出版日期:2022-04-22 发布日期:2022-04-25
  • 通讯作者: 徐亮,闫妍
  • 基金资助:
    中国检验检疫科学研究院基础科研业务费项目(2019JK036)

Effect of Staphylococcus epidermidis fermented extract on human skin barrier

Tie Hang1,4,Lv Yufeng2,Zhang Yang3,Xu Liang4,*(),Yan Yan1,*()   

  1. 1. Chinese Academy of Inspection and Quarantine, Beijing 100176, China
    2. Tianjin University of Commerce, Tianjin 300000, China
    3. SHIN ( Shanghai) E-commerce Co., Ltd., Shanghai 200000, China
    4. School of Pharmacy, Tianjin Medical University, Tianjin 300070, China
  • Received:2021-06-21 Revised:2021-03-23 Online:2022-04-22 Published:2022-04-25
  • Contact: Liang Xu,Yan Yan

摘要:

从健康人体皮肤中分离获取一株表皮葡萄球菌(SE),并进行培养发酵,制备SE发酵提取物;通过实时无标记动态细胞分析技术(RTCA)检测SE发酵提取物对人原代角质形成细胞(KC)的活性影响;使用聚肌苷酸胞苷酸(poly(I:C))和脂多糖(LPS)分别刺激KC细胞,造成Toll样受体(TLR)3和TLR 4介导的炎症反应模型,然后加入SE发酵提取物,研究SE发酵提取物对KC细胞炎症反应的抑制作用;最后使用十二烷基硫酸钠(SLS)刺激3D表皮模型,构建受损皮肤模型,并研究SE发酵提取物对损伤皮肤模型中丝聚蛋白(FLG)和兜甲蛋白(LOR)表达影响的作用。结果显示,体积分数为1% SE发酵提取物作用于KC细胞100 h,并未见明显细胞毒性作用,且具有一定的保护作用,SE发酵提取物可以抑制由TLR3介导的炎症反应,而对TLR4介导的炎症反应无明显抑制作用,并且可以有效地提高受损屏障中的FLG和LOR含量。表皮葡萄球菌作为人体皮肤的常驻菌,对皮肤的屏障具有重要的积极作用。

关键词: 表皮葡萄球菌, 炎症因子, 皮肤屏障, 3D皮肤模型

Abstract:

A large number of studies have shown that Staphylococcus epidermidis (SE), one of the normal microflora colonized in human skin and mucous membrane, plays a positive role in human skin barrier health. Here we reveal a new mechanism which a product of Staphylococcus epidermidis can enhance the skin barrier function. This enhancement is mediated by a Staphylococcus epidermidis fermentation extract isolated from healthy human skin in our laboratory, and acts on keratinocytes (KC) as well as 3D epidermal model triggered through Toll-like receptor (TLR) 3, filaggrin (FLG) and loricin (LOR), respectively. The effect of SE fermentation extract on human keratinocytes was detected by Real Time Cellular Analysis (RTCA). Polyinosinic-polycytidylic acid (poly (I:C)) and lipopolysaccharide (LPS) were used to stimulate KC cells respectively, causing TLR3 and TLR4-mediated inflammatory response models, and then added SE fermentation extract to study the inhibitory effects of SE fermentation extract on the inflammatory KC cells. Finally, sodium lauryl sulfate (SLS) was used to stimulate the 3D epidermal model to construct a damaged skin model, and to study the effect of SE fermentation extract on the expression of filaggrin (FLG) and loricin (LOR) in the damaged skin model. The results show that 1% (φ) SE fermentation extract acts on KC cells for 100 hours, and no obvious cytotoxicity is seen, which has a certain protective effect on cell activity. From this we can predict that SE may have some effect on the function of human epidermis, for example, enhancing the skin barrier function. SE fermentation extract can inhibit the inflammatory response mediated by TLR3, but has no obvious inhibitory effect on the inflammatory response mediated by TLR4. We speculate that Staphylococcus epidermidis fermentation extract may affect the inflammatory response of keratinocytes through the negative regulation of TLR signal. The results of the 3D skin model show that the SE fermented extract can effectively increase the FLG and LOR content in the damaged barrier. These findings show that Staphylococcus epidermidis, as a resident bacteria of human skin, can modulate specific cutaneous inflammatory responses and enhance the skin barrier function through research on keratinocytes and 3D epidermal model after wounding.

Key words: Staphylococcus epidermidis, inflammatory cytokines, skin barrier, 3D skin model

中图分类号: 

  • TQ658