EGCG柔性脂质体的制备及其透皮性能研究

doi:10.3969/j.issn.1001-1803.2021.05.003

摘要/Abstract

摘要：

采用边缘活化剂修饰EGCG脂质体,以粒径、包封率和产率为指标,考察边缘活化剂种类和添加量对EGCG柔性脂质体的影响。结果表明,以Tween 80为边缘活化剂,添加量为50%（占卵磷脂质量比）时,粒径、包封率、产率较优,分别为（77.8±1.40） nm、85.18%±1.22%、96.77%±1.47%。进一步通过皮肤渗透实验证明,EGCG柔性脂质体拥有比脂质体更好的透皮效果,24 h透皮含量为21.13 μg/cm²,明显高于EGCG脂质体,此结果与荧光显微镜观察的结果一致。最终储藏稳定性实验表明,相比EGCG脂质体,EGCG柔性脂质体稳定性更佳,4 ℃避光保存60 d保留率高达88.24%。

关键词: EGCG, 柔性脂质体, 透皮运输, 稳定性

Abstract:

EGCG flexible liposomes were prepared by modifying the EGCG liposomes with edge activators. The effects of the type and dosage of edge activators on the particle size, encapsulation rate and yield of EGCG flexible liposomes were investigated. The results showed that when Tween 80 was used as the edge activator and the dosage was 50% （the mass ratio to lecithin）, the particle size, encapsulation rate and yield were better, which were （77.8±1.40） nm, 85.18%±1.22% and 96.77%±1.47%, respectively. The transdermal experiment showed that the EGCG flexible liposomes exhibited better transdermal effect than the liposomes. The transdermal content of EGCG flexible liposomes was 21.13 μg/cm² after 24 h, which was significantly higher than EGCG liposomes. It was consistent with the results of fluorescence labeling experiment. Storage stability experiments showed that, compared with EGCG liposomes, the EGCG flexible liposomes were more stable, and the retention rate was up to 88.24% after being stored in the dark at 4 ℃ for 60 days.

Key words: EGCG, flexible liposome, skin penetration, stability

中图分类号:

TQ658

陈凤凤,曹振大,梁蓉. EGCG柔性脂质体的制备及其透皮性能研究[J]. 日用化学工业, 2021, 51(5): 390-395.

CHEN Feng-feng,CAO Zhen-da,LIANG Rong. Study on the preparation and transdermal performance of EGCG flexible liposome[J]. China Surfactant Detergent & Cosmetics, 2021, 51(5): 390-395.

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