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日用化学工业（中英文）

日用化学工业 ›› 2021, Vol. 51 ›› Issue (5): 396-401.doi: 10.3969/j.issn.1001-1803.2021.05.004

• 开发与应用 • 上一篇    下一篇

常用表面活性剂对唾液链球菌与食窦魏斯氏口腔益生菌的影响

王雁雯1(),王亚静1(),赵鑫2(),高迪1,叶相印1,黄赞扬1,王晓宇1,王宇1   

  1. 1.天津中医药大学 现代中药发现与制剂技术教育部工程研究中心,天津 301617
    2.天津中医药大学 方剂学教育部重点实验室,天津 301617
  • 收稿日期:2020-07-25 修回日期:2021-03-22 出版日期:2021-05-22 发布日期:2021-05-21
  • 通讯作者: 王亚静,赵鑫
  • 作者简介:王雁雯（1996-）,女,天津人,硕士研究生,电话：18822137690,E-mail： 18822137690@163.com。
  • 基金资助:
    国家“重大新药创制”科技重大专创(2018ZX09721-005);天津市科技计划项目(18ZXXYSY00130);天津市科技计划项目(19ZYPTJC00060);天津市自然基金青年项目(18JCQNJC83300)

Effects of commonly used surfactants on Streptococcus salivarius and Weissella oral probiotics

WANG Yan-wen1(),WANG Ya-jing1(),ZHAO Xin2(),GAO Di1,YE Xiang-yin1,HUANG Zan-yang1,WANG Xiao-yu1,WANG Yu1   

  1. 1. Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education,Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
    2. Key Research Laboratory of Prescription Compatibility among Components, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
  • Received:2020-07-25 Revised:2021-03-22 Online:2021-05-22 Published:2021-05-21
  • Contact: Ya-jing WANG,Xin ZHAO

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摘要：

以PEG-40氢化蓖麻油（RH-40）、PEG-60氢化蓖麻油（RH-60）、椰油酰胺丙基甜菜碱（CAB-35）、月桂酰肌氨酸钠（LS-97）、椰油酰甘氨酸钠（AK-301）、十二烷基硫酸钠（SDS） 6种口腔护理产品常用表面活性剂为研究对象,以食窦魏斯氏菌和唾液链球菌为实验益生菌菌株,探究不同质量浓度表面活性剂对口腔益生菌生长的影响。改良牛津杯法结果表明,益生菌对质量浓度10 g/L的6种表面活性剂均敏感;微量肉汤稀释法测定结果显示,不同质量浓度的不同表面活性剂对口腔益生菌的影响不同。质量浓度≤0.078 g/L的RH-60,质量浓度≤0.156 g/L的RH-40对益生菌的生长具有促进作用;0.156~0.625 g/L的RH-60,0.02 g/L的CAB-35,质量浓度≤0.039 g/L的LS-97、AK-301对益生菌的生长无显著影响;而质量浓度≥0.313 g/L的SDS、LS-97、AK-301,质量浓度≥1.25 g/L的CAB-35则完全抑制益生菌的生长。质量浓度>0.156 g/L的RH-60、RH-40比相同质量浓度其余表面活性剂对益生菌的抑制作用弱。低质量浓度的AK-301、CAB-35、LS-97,各质量浓度RH-60、RH-40可优先考虑应用于口腔清洁护理产品中。

关键词: 表面活性剂, 食窦魏斯氏菌, 唾液链球菌, 益生菌, 时间-生长曲线

Abstract:

The effects of different mass concentrations of surfactants on the growth of oral probiotics were studied. Six surfactants commonly used in oral care products, i.e. PEG-40 hydrogenated castor oil （RH-40） , PEG-60 hydrogenated castor oil （RH-60） , cocamidopropyl betaine （CAB-35） , sodium lauroyl sarcosinate （LS-97） ,sodium cocoyl glycinate （AK-301） and sodium dodecyl sulfate （SDS） , were used; Weissella cibaria and Streptococcus salivarius were used as experimental probiotic strains. The results of modified Oxford cup method showed that probiotics were all sensitive to the six surfactants with a mass concentration of 10 g/L; the results of broth micro-dilution method showed that different mass concentrations of different surfactants had different effects on oral probiotics. RH-60 with a mass concentration ≤0.078 g/L and RH-40 with a mass concentration ≤0.156 g/L could promote the growth of probiotics; RH-60 with a mass concentration of 0.156-0.625 g/L, CAB-35 with a mass concentration of 0.02 g/L, and LS-97 and AK-301 with mass concentrations ≤0.039 g/L had no significant effect on the growth of probiotics; SDS, LS-97 and AK-301 with mass concentrations ≥0.313 g/L and CAB-35 with a mass concentration ≥1.25 g/L completely inhibited the growth of probiotics. The inhibitory effects of RH-60 and RH-40 with mass concentrations >0.156 g/L on probiotics were weaker than other surfactants at the same mass concentration. Low mass concentrations of AK-301, CAB-35 and LS-97, and each mass concentration of RH-60 and RH-40, could be given priority to be used in oral care products.

Key words: surfactant, Weissella cibaria, Streptococcus salivarius, probiotic, time-growth curve

中图分类号: 

  • TQ423