复方紫草凝胶外用制剂的抗炎与抑菌作用研究

doi:10.3969/j.issn.1001-1803.2020.12.006

摘要/Abstract

摘要：

采用三元相图法筛选复方紫草凝胶外用制剂（CRAGTP）处方;使用3种急性炎症模型：二甲苯致小鼠耳肿胀、二甲苯和醋酸增加毛细血管通透性研究CRAGTP的抗炎作用;K-B纸片扩散法研究了CRAGTP对金黄色葡萄球菌（Staphylococcus aureus, SA）、大肠杆菌（Escherichia coli, E.coli）、铜绿假单胞菌（Pseudomonas areuginas, PA）的体外抑菌作用。结果显示,CRAGTP处方为复方紫草提取物0.5%、油相26.0%、表面活性剂40.2%、助表面活性剂（甘油）3.3%、水30.0%,外观澄清透明,pH=6.49,粒径为22.64 nm。CRAGTP能抑制二甲苯致急性炎症（P<0.01）、醋酸致急性炎症（P<0.05）;CRAGTP对SA和E.coli有抑制作用,对PA几乎没有抑制作用。CRAGTP抗炎作用良好,有一定的抑菌作用。

关键词: 复方紫草凝胶外用制剂, 表面活性剂, 炎症, 抑菌, 二甲苯

Abstract:

The optimal formulation of compound radix arnebiae gel topical preparation （CRAGTP） was screened by ternary phase diagram. Three acute inflammation models （xylene-induced ear swelling, xylene-induced abdominal skin capillary permeability increase, and acetic acid-induced abdominal cavity capillary permeability increase） were used to study the anti-inflammatory effects of CRAGTP. Disc diffusion test （K-B method） was used to study the inhibitory effect of CRAGTP on Staphylococcus aureus （SA）, Escherichia coli （E.coli） and Pseudomonas areuginas （PA） in vitro. Preliminary results show that the optimal composition of CRAGTP formulation is 0.5% of compound radix arnebiae extract, 26.0% of oil phase mixture, 40.2% of surfactants, 3.3% of glycerin and 30.0% of water. The formulated gel is clear and transparent, with pH of 6.49 and particle size of 22.64 nm. CRAGTP shows great ability to inhibit the xylene-induced ear swelling （P<0.01）, xylene-induced increasing of abdominal skin capillary permeability （P<0.01）, and acetic acid-induced increasing of abdominal cavity capillary permeability （P<0.05）. It has an inhibitory effect on SA and E.coli, but has little inhibitory effect on PA. CRAGTP has a good anti-inflammatory effect and a certain antibacterial activity.

Key words: compound radix arnebiae gel topical preparation, surfactant, inflammation, antibacterial activity, xylene

中图分类号:

TQ423

贾颖,王佳敏,王世晖,刘凯丽,杨大伟,王艳. 复方紫草凝胶外用制剂的抗炎与抑菌作用研究[J]. 日用化学工业, 2020, 50(12): 848-853.

JIA Ying,WANG Jia-min,WANG Shi-hui,LIU Kai-li,YANG Da-wei,WANG Yan. Study on the anti-inflammatory and antibacterial effects of compound radix arnebiae gel topical preparation[J]. China Surfactant Detergent & Cosmetics, 2020, 50(12): 848-853.

图/表 9

表 1

表 2

图 1

表 3

表 4

图 2

表 5

表 6

表 7

参考文献 16

[1]	Zhang Manning, Liu Jiangmei, Liu Huimin, et al. Optimization of extraction process of natural dye from arnebiae euchroma (royle) johnst[J]. Progress in Textile Science & Technology, 2017 (5) : 9-22.
[2]	Chinese Pharmacopoeia Commission. The pharmacopoeia of People’s Republic of China, volume 1, 2015 [S]. Beijing: China Medical Science Press, 2015: 340.
[3]	Zhao Xianmin, Li Nan, Song Yanli, et al. Chemical constituents of arnebiae radix[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016,22(15) : 47-50.
[4]	Zhao Yuan, Wan Kaihua, Wen Youwu, et al. Study on the pharmacodynamic of Zi-Cao-San-Huang suppository[J]. Journal of Xinjiang Medical University, 2018,41(7) : 883-885.
[5]	Zheng Hengguang, Weng Minjie, Tang Baosha, et al. Research progress on health promotion and disease prevention of olive oil[J]. Food Science and Technology, 2019,44(10) : 196-199.
[6]	Li Chunxia. Application of jojoba oil in cosmetics[J]. Detergent & Cosmetics, 2007 (2) : 35-36.
[7]	Huang Ying. Research progress of natural drug gels[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy, 2014,23(15) : 35-36, 40.
[8]	Yang Ming. P-PGBL can inhibit the inflammatory response through TLR4/NF-κB signaling pathway[D]. Changchu: Jilin University, 2014.
[9]	Li Tan, Chen Hong, Bai Shufen, et al. Immunomodulatory activity of artesunate to T iymphocytes from the spleens of delayed-type hypersensitivity mice[J]. Chinese Journal of Tissue Engineering Research, 2012,16(5) : 843-846.
[10]	Li Chen, Xiao Fei, Chang Ke, et al. Effect of compound comeria medica oil on acute inflammation in young mice[J]. Pharmacology and Clinics of Chinese Materia Medica, 2018,34(3) : 172-175.
[11]	Yang Qiaofang, Meng Qinggang. Discuss the animal model about inflammation[J]. Chinese Archives of Traditional Chinese Medicine, 2008 (3) : 516-517.
[12]	Jia Ying, Zhao Jing, Feng Qianjin, et al. Study on extraction, antioxidant activity and antibacterial activity of total flavonoids from Compound Astragalus[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2020,35(8) : 3858-3863.
[13]	Zhang Zesheng, Gao Shan, Guo Qing, et al. Processing and comprehensive utilization of palmitoleic acid[J]. China Food Additives, 2016 (9) : 198-202.
[14]	Yang Tongqun, Zhang Qian, Geng Xilin, et al. Transcriptomic analysis of gene alteration related to the regulation of inflammation/immune responses after acute myoinjury[J]. Chinese Journal of Clinical Anatomy, 2015,33(2) : 171-175.
[15]	Du lijun, Gao Yunfeng, Jin Guoliang, et al. Anti-infection effect of Broussonetia papyrifera leaves extraction on xylene-induced inflammation in mice[J]. Journal of Community Medicine, 2018,16(22) : 1642-1645.
[16]	Meng Ning, Huang Shan, Hu Dandan, et al. Anti-inflammatory and analgesic effects of different solvent extractions of Nepeta Angustifolia C. Y.Wu [J]. Chinese Archives of Traditional Chinese Medicine, 2018,36(8) : 1874-1877.

类别	辅料
油相	橄榄油、霍霍巴油
表面活性剂	聚氧乙烯40氢化蓖麻油（RH 40）、司班80（Span 80）
助表面活性剂	无水乙醇、甘油、PEG-400

无抑菌作用	有抑菌作用	敏感程度
	6≤d<10	低度敏感
d<6	10≤d<15	中度敏感
	15≤d	高度敏感

组别	剂量/mg	耳肿胀度/mg	耳肿胀抑制率/%
空白组	40	0.25±0.01^**	—
模型组	40	3.29±0.81^△△	—
阳性药组	40	1.49±0.76^**	54.71
空白凝胶组	40	1.90±0.69^**	42.25
CRAGTP组	40	0.83±0.45^**#	74.77

组别	剂量/（mg·cm^-2）	吸光度值	组别	剂量/（mg·cm^-2）	吸光度值
空白组	45	0.042±0.008^**	空白凝胶组	45	0.065±0.006^**
模型组	45	0.224±0.079^△△	CRAGTP组	45	0.052±0.018^**
阳性药组	45	0.051±0.017^**

组别	剂量/（mg·cm^-2）	吸光度值
空白组	45	0.290±0.031^*
模型组	45	0.496±0.099^△
阳性药组	45	0.352±0.071
空白凝胶组	45	0.385±0.089
CRAGTP组	45	0.212±0.109^*