海藻酸钠对酪氨酸酶的抑制作用

doi:10.3969/j.issn.1001-1803.2019.06.007

日用化学工业 ›› 2019, Vol. 49 ›› Issue (6): 388-392.doi: 10.3969/j.issn.1001-1803.2019.06.007

海藻酸钠对酪氨酸酶的抑制作用

郑曦¹,陈智多²,张德蒙³,秦益民³,易彩霞¹,黄啸^1,³()

1. 铜仁学院大健康学院,贵州铜仁 554300
2. 铜仁学院材料与化学工程学院,贵州铜仁 554300
3. 青岛明月海藻集团有限公司海藻活性物质国家重点实验室,山东青岛 266400

收稿日期:2018-10-14 修回日期:2019-05-28 出版日期:2019-06-22 发布日期:2019-06-24
通讯作者: 黄啸
作者简介:郑曦（1988-）,女,重庆渝北人,助教,电话：（0856）8121809,E-mail： zhengxi8420@126.com。
基金资助:
海藻活性物质国家重点实验室开放基金资助项目(SKL-BASS1719);贵州省教育厅青年科技人才成长项目(黔教合KY字[2017]311);铜仁市科技计划项目(铜市科研[2018]19号)

Inhibitory effect of sodium alginate on the activity of tyrosinase

ZHENG Xi¹,CHEN Zhi-duo²,ZHANG De-meng³,QIN Yi-min³,YI Cai-xia¹,HUANG Xiao^1,³()

1. School of Sports and Health Science, Tongren University, Tongren, Guizhou 554300, China
2. College of Material and Chemical Engineering, Tongren University, Tongren, Guizhou 554300, China
3. State Key Laboratory of Bioactive Seaweed Substances, Qingdao Brightmoon Seaweed Group Co Ltd, Qingdao, Shandong 266400, China

Received:2018-10-14 Revised:2019-05-28 Online:2019-06-22 Published:2019-06-24
Contact: Xiao HUANG

摘要/Abstract

摘要：

通过酶抑制动力学实验考察了海藻酸钠在实验体系和黑素细胞内对酪氨酸酶和黑色素生成的抑制作用,利用荧光光谱分析海藻酸钠对酪氨酸酶抑制作用机理,同时考察海藻酸钠对黑素细胞的毒性。结果表明,在64 mmol/L浓度内,细胞活性均在90%以上,显示出良好的生物相容性。海藻酸钠对酪氨酸酶活性及黑色素产量均具有一定的抑制效果,且抑制类型在20 mmol/L浓度内呈明显的浓度依赖型。海藻酸钠与酪氨酸酶相互作用后,对其内源性荧光产生了淬灭作用。酪氨酸酶的最大发射波长出现了红移,表明海藻酸钠与酪氨酸酶相互作用时,改变了其氨基酸残基的疏水性环境。

关键词: 美白化妆品添加剂, 海藻酸钠, 酪氨酸酶, 抑制剂, 抑制机理

Abstract:

Enzyme inhibition kinetics experiments were carried out in vitro and in melanocytes to study the inhibitory effect of sodium alginate on the activity of tyrosinase and melanin deposition. The inhibition mechanism of sodium alginate on tyrosinase was analyzed by fluorescence spectroscopy, and the toxicity of sodium alginate on melanocyte was also investigated. The results show that within the concentration of 64 mmol/L, the cell viability was above 90%, indicating good biocompatibility. Sodium alginate had a certain inhibitory effect on tyrosinase activity and melanin production, which was in a concentration-dependent manner below the concentration of 20 mmol/L. The endogenous fluorescence of tyrosinase was partly quenched after interacting with sodium alginate. The redshift of the maximum emission wavelength appearing in the fluorescence spectrum of tyrosinase indicated that the interaction between sodium alginate and tyrosinase changed the hydrophobic environment of its amino acid residues.

Key words: whitening cosmetic additive, sodium alginate, tyrosinase, inhibitor, inhibitioon mechanism

中图分类号:

TQ658

郑曦,陈智多,张德蒙,秦益民,易彩霞,黄啸. 海藻酸钠对酪氨酸酶的抑制作用[J]. 日用化学工业, 2019, 49(6): 388-392.

ZHENG Xi,CHEN Zhi-duo,ZHANG De-meng,QIN Yi-min,YI Cai-xia,HUANG Xiao. Inhibitory effect of sodium alginate on the activity of tyrosinase[J]. China Surfactant Detergent & Cosmetics, 2019, 49(6): 388-392.

图/表 5

参考文献 23

[1]	Jeon N, Kim Y, Kim E , et al. Inhibitory effect of carvacrol on melanin synjournal via suppression of tyrosinase expression[J]. Journal of Functional Foods, 2018,45:199-205. doi: 10.1016/j.jff.2018.03.043
[2]	Promden W, Viriyabancha W, Monthakantirat O , et al. Correlation between the potency of flavonoids on mushroom tyrosinase inhibitory activity and melanin synjournal in melanocytes[J]. Molecules, 2018,23(6) : 1403. doi: 10.3390/molecules23061403
[3]	Hwang J, Park N, Na Y , et al. Coumestrol down-regulates melanin production in melan-a murine melanocytes through degradation of tyrosinase[J]. Biological & Pharmaceutical Bulletin, 2017,40(4) : 535-539.
[4]	Abbas Q, Raza H, Hassan M , et al. Acetazolamide inhibits the level of tyrosinase and melanin:An enzyme kinetic, in vitro, in vivo, and in silico studies[J]. Chemistry & Biodiversity, 2017,14(9) : 1700117.
[5]	Garcia-Gavin J, Gonzalez-Vilas D, Fernandez-Redondo V , et al. Pigmented contact dermatitis due to kojic acid: A paradoxical side effect of a skin lightener[J]. Contact Dermatitis, 2010,62(1) : 63-64. doi: 10.1111/cod.2010.62.issue-1
[6]	Mata T, Sanchez J, Oyanguren J . Allergic contact dermatitis due to kojic acid[J]. Dermatitis, 2005,16(2) : 89.
[7]	Chen C, Lin L, Yang W , et al. An updated organic classification of tyrosinase inhibitors on melanin biosynjournal[J]. Current Organic Chemistry, 2015,19(1) : 4-18. doi: 10.2174/1385272819666141107224806
[8]	Gasowska-Bajger B, Wojtasek H . Reactions of flavonoids with o-quinones interfere with the spectrophotometric assay of tyrosinase activity[J]. Journal of Agricultural and Food Chemistry, 2016,64(26) : 5417-5427. doi: 10.1021/acs.jafc.6b01896
[9]	Lee H, Lee W, Chang S , et al. Hesperidin, a popular antioxidant inhibits melanogenesis via Erk1/2 mediated MITF degradation[J]. International Journal of Molecular Sciences, 2015,16(8) : 18384-18395. doi: 10.3390/ijms160818384
[10]	Magid A, Voutquenne-Nazabadioko L, Bontemps G , et al. Tyrosinase inhibitors and sesquiterpene diglyclosides from Guioa villosa[J]. Planta Medica, 2008,74(9) : 55-60. doi: 10.1055/s-2007-993780
[11]	Gardelly M, Trimech B, Belkacem M , et al. Synjournal of novel diazaphosphinanes coumarin derivatives with promoted cytotoxic and anti-tyrosinase activities[J]. Bioorganic & Medicinal Chemistry Letters, 2016,26(10) : 2450-2454.
[12]	Gomes A, Mano J, Queiroz J , et al. Incorporation of antimicrobial peptides on functionalized cotton gauzes for medical applications[J]. Carbohydrate Polymers, 2015,127:451-461. doi: 10.1016/j.carbpol.2015.03.089
[13]	Schleeh T, Madau M, Roessner D . Two competing reactions of tetrabutylammonium alginate in organic solvents:Amidation versus gamma-lactone synjournal[J]. Carbohydrate Polymers, 2016,138:244-251. doi: 10.1016/j.carbpol.2015.11.070
[14]	Kim H, Song M, Lee E , et al. Injectable hydrogels derived from phosphorylated alginic acid calcium complexes[J]. Materials Science and Engineering C, 2015,51:139-147. doi: 10.1016/j.msec.2015.02.031
[15]	Vaid U, Mittal S, Babu J . Influence of anion induced proton abstraction on Cu (II) adsorption by alginic acid[J]. Reactive and Functional Polymers, 2015,97(196) : 48-55. doi: 10.1016/j.reactfunctpolym.2015.10.006
[16]	Xie L, Chen Q, Huang H , et al. Inhibitory effects of some flavonoids on the activity of mushroom tyrosinase[J]. Biochemisty, 2003,68(4) : 481-491.
[17]	Aguero L, Zaldivar-Silva D, Pena L , et al. Alginate microparticles as oral colon drug delivery device:A review[J]. Carbohydrate Polymer, 2017,168:32-24. doi: 10.1016/j.carbpol.2017.03.033
[18]	Reakasame S, Boccaccini A . Oxidized alginate-based hydrogels for tissue engineering applications:A review[J]. Biomacromolecules, 2018,19(1) : 3-21. doi: 10.1021/acs.biomac.7b01331
[19]	Zhou Q, Kang H, Bielec M , et al. Influence of different divalent ions cross-linking sodium alginate-polyacrylamide hydrogels on antibacterial properties and wound healing[J]. Carbohydrate Polymers, 2018,197:292-304. doi: 10.1016/j.carbpol.2018.05.078
[20]	Da Costa J, Rodrigues A, Farias L , et al. Biological effects of kojic acid on human monocytes in vitro[J]. Biomedicine & Pharmacotherapy, 2018,101:100-106.
[21]	Higa Y, Kawabe M, Nabae K , et al. Kojic acid -absence of tumor-initiating activity in rat liver, and of carcinogenic and photo-genotoxic potential in mouse skin[J]. The Journal of Toxicological Science, 2007,32:143-159. doi: 10.2131/jts.32.143
[22]	Kus N, Dolinska M, Young K , et al. Membrane-associated human tyrosinase is an enzymatically active monomeric glycoprotein[J]. Plos One, 2018,13(6) : 198247.
[23]	Chai W, Lin M, Song F , et al. Rifampicin as a novel tyrosinase inhibitor:Inhibitory activity and mechanism[J]. International Journal of Biological Macromolecules, 2017,102:425-430. doi: 10.1016/j.ijbiomac.2017.04.058

海藻酸钠对酪氨酸酶的抑制作用

Inhibitory effect of sodium alginate on the activity of tyrosinase

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 23

相关文章 15

Metrics

本文评价

推荐阅读 10

[1]	李宁, 李恩念, 陈红波, 程芳, 邹衡芳, 陈鸿鹏. 祛斑美白类化妆品功效成分的研究现状[J]. 日用化学工业（中英文）, 2024, 54(1): 80-89.
[2]	王雪娇, 杜丽娟, 徐元喜. 液体洗涤蛋白酶稳定剂筛选方法开发[J]. 日用化学工业（中英文）, 2023, 53(7): 796-801.
[3]	郭芳钰, 韩婷婷, 王晓娜, 陈玉荣, 王晓梅, 杨素珍. 中药双向发酵液制备及其抗衰老、保湿、美白功效研究[J]. 日用化学工业（中英文）, 2023, 53(5): 523-531.
[4]	潘继飞, 王晓娜, 郭海姣, 杨素珍, 陈建英. 5α-还原酶抑制剂体外评价体系的建立及其应用[J]. 日用化学工业（中英文）, 2023, 53(11): 1280-1284.
[5]	毛金超,隋振全,徐桂云,范金石. 天然生物质材料的制备、性质与应用（Ⅱ）——pH敏感型功能海洋多糖：海藻酸钠[J]. 日用化学工业, 2022, 52(2): 124-133.
[6]	张凯强,许虎君. 二葡糖基没食子酸的合成及其性能研究[J]. 日用化学工业, 2022, 52(2): 140-146.
[7]	查雨锋,黄加文,詹易,李婷,颜宏,吴德松. 白梅花提取物抗氧化及美白功效评价[J]. 日用化学工业, 2022, 52(2): 172-179.
[8]	何瑞源,王硕,韦桂丽,龚小妹,吴佩莹,缪剑华. 壮药菲牛蛭提取物的美白作用及其机制初探[J]. 日用化学工业, 2022, 52(1): 35-43.
[9]	任倩倩,吴华,金建明. 化妆品植物原料（Ⅱ）——抑制酪氨酸酶活性的植物美白原料的研究与开发[J]. 日用化学工业, 2021, 51(3): 178-185.
[10]	王莹,唐寅,吴亚妮. 复方精油对小鼠B16细胞内黑色素生成影响[J]. 日用化学工业, 2021, 51(3): 214-219.
[11]	罗天骥,梅子坤,宋京九,祝钧. 异阿魏酸酰胺类衍生物的合成及其美白活性的研究[J]. 日用化学工业, 2021, 51(11): 1102-1108.
[12]	王勇恒,梁彦会,孙培冬. 川芎提取物美白双靶点活性研究[J]. 日用化学工业, 2020, 50(4): 249-254.
[13]	王飞飞,王敏竹,王晗,黄山,旦增江白,李斌. 藏荆芥提取物对酪氨酸酶活性及其动力学研究[J]. 日用化学工业, 2019, 49(5): 315-319.
[14]	李志英,王雪寒,高瑞苑. 微波提取白茯苓美白成分的工艺研究[J]. 日用化学工业, 2019, 49(4): 238-243.
[15]	郝谜谜, 王艳, 刘园园, 鲍炎, 李红艳, 郑琳. 苯乙基间苯二酚抑制黑色素形成的机理研究[J]. 日用化学工业, 2018, 48(5): 293-298.