发酵法制备沙棘籽粕总黄酮的初步纯化及抗衰老活性评价

doi:10.3969/j.issn.1001-1803.2019.10.006

日用化学工业 ›› 2019, Vol. 49 ›› Issue (10): 661-667.doi: 10.3969/j.issn.1001-1803.2019.10.006

发酵法制备沙棘籽粕总黄酮的初步纯化及抗衰老活性评价

由士权¹,王昌涛^1,²(),张佳婵¹,李萌¹,赵丹¹,安全³

1. 北京工商大学植物资源研究开发北京市重点实验室,北京 100048
2. 北京工商大学北京食品营养与人类健康高精尖创新中心,北京 100048
3. 云南白药集团健康产品有限公司,云南昆明 650500

收稿日期:2018-07-09 修回日期:2019-08-23 出版日期:2019-10-22 发布日期:2019-10-24
通讯作者: 王昌涛
作者简介:由士权（1994-）,男,硕士研究生,电话：13269182262,E-mail： 13269182262@163.com。
基金资助:
北京工商大学两科基金培育项目(LKJJ2016-09);北京工商大学学位与研究生教育研究项目(2017XWYJS014)

Preliminary purification and anti-aging activity evaluation of total flavonoids prepared from sea-buckthorn seed meal by fermentation

YOU Shi-quan¹,WANG Chang-tao^1,²(),ZHANG Jia-chan¹,LI Meng¹,ZHAO Dan¹,AN Quan³

1. Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing 100048, China
2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China
3. Yunnan Baiyao Group Health Products Co. Ltd., Kunming, Yunnan 650500, China

Received:2018-07-09 Revised:2019-08-23 Online:2019-10-22 Published:2019-10-24
Contact: Chang-tao WANG

摘要/Abstract

摘要：

研究大孔吸附树脂纯化沙棘籽粕发酵提取液的工艺条件以及抗衰老活性。对3种大孔吸附树脂分离纯化的效果进行了比较,考察了X-5大孔树脂分离的较佳工艺条件,并通过MTT法评价了提取物对细胞生长的影响,采用DPPH法、提取物对细胞内活性氧和MMP-1活性的抑制、促进胶原蛋白合成来评价提取物的抗衰老活性。结果表明,较佳工艺条件为：吸附时间2 h,吸附温度15 ℃,洗脱剂体积分数60%,洗脱时间2.5 h,洗脱温度45 ℃。在此条件下吸附量可达2.33 mg/g,解析率可达94.75%;MTT实验表明对成纤维细胞基本无毒副作用;对DPPH自由基清除率达到50%时的质量浓度（IC₅₀）为1.06 mg/L,活性氧水平显著下降,MMP-1活性降低,胶原蛋白含量有所提高。沙棘籽粕发酵液有较好的抗衰老活性。

关键词: 化妆品添加剂, 沙棘籽粕, 黄酮, 发酵, 抗衰老

Abstract:

The fermentation extract of sea-buckthorn seed meal was purified by macroporous adsorption resin. The technological conditions in the purification process were studied, as well as the anti-aging activity of the extract. The effects of separation and purification of three macroporous adsorption resins were compared. The optimum separation conditions with X-5 macroporous resin were investigated. The effect of the extract on cell growth was evaluated by MTT method. The anti-aging activity of the extract was evaluated by DPPH method, the inhibition of the activities of intracellular reactive oxygen species and MMP-1, and the promotion of collagen synthesis. The results show that the optimum process conditions: adsorption time of 2 h, adsorption temperature of 15 ℃, eluent volume fraction of 60%, elution time of 2.5 h, and elution temperature of 45 ℃. Under such conditions, the adsorption amount could reach 2.33 mg/g, and the resolution rate could reach 94.75%. The MTT experiment showed that it had no toxic side effects on fibroblasts; the mass concentration when the DPPH radical scavenging rate reaches 50%（IC₅₀）was 1.06 mg/L; the level of reactive oxygen species decreased significantly; the MMP-1activity was decreased; the collagen content was increased. The fermentation broth of sea-buckthorn seed meal showed good anti-aging activity.

Key words: additive of cosmetics, sea-buckthorn seed meal, flavonoid, fermentation, anti-aging

中图分类号:

TQ658

由士权,王昌涛,张佳婵,李萌,赵丹,安全. 发酵法制备沙棘籽粕总黄酮的初步纯化及抗衰老活性评价[J]. 日用化学工业, 2019, 49(10): 661-667.

YOU Shi-quan,WANG Chang-tao,ZHANG Jia-chan,LI Meng,ZHAO Dan,AN Quan. Preliminary purification and anti-aging activity evaluation of total flavonoids prepared from sea-buckthorn seed meal by fermentation[J]. China Surfactant Detergent & Cosmetics, 2019, 49(10): 661-667.

图/表 14

表 1

表 2

图 1

表 3

表 4

图 2

图 3

图 4

图 5

图 6

图 7

图 8

图 9

图 10

参考文献 19

[1]	Zhao E L, Zhang H R, Gai Q Q , et al. Determination of seabuckthorn flavonoids and its anti-aging effect[J]. Chemical Research and Application, 2003,15(2):284-285.
[2]	Ushakova N A, Brodskii E S, Kozlova A A , et al. Anaerobic solid-phase fermentation of plant substrates by Bacillus subtilis[J]. Applied Biochemistry and Microbiology, 2007,45(1):61-67.
[3]	An J L, An L J, Liu J H . Study on active and medicinal ingredients in seabuckthorn oil[J]. Journal of Chemical Industry & Engineering, 2004,25(3):1-3.
[4]	Peng L, Tang M, Hu X M , et al. Progress of flavonoids ectraction from seabuckthorn[J]. Natural Product Research and Development, 2007,45(1):61-67.
[5]	Liu L S . Differential response of NHEKs and HaCaT cells to hydrogen peroxide-induced oxidative stress and the defense mechanism study of against oxidative stress-incluced senescence in NHSFs[D]. Changsha: Central South University, 2012.
[6]	Michel T, Destandaua E, Lucchesi M E , et al. Antimicrobial, antioxidant and phytochemical investigations of sea buckthorn(Hippophaë rhamnoides L.) leaf, stem, root and seed[J]. Food Chemistry, 2012,131(3):754-760.
[7]	Górnaś P, Šnē E, Siger A , et al. Sea buckthorn (Hippophae rhamnoides L.) vegetative parts as an unconventional source of lipophilic antioxidants[J]. Saudi Journal of Biological Sciences, 2016,23(4):512-516.
[8]	Rodrigues M J, Neves V, Martins A , et al. In vitro antioxidant and anti-inflammatory properties of Limonium algarvense flowers’ infusions and decoctions: A comparison with green tea (Camellia sinensis)[J]. Food Chemistry, 2016,200:322-329.
[9]	Shi D D. Precess Optimization of the extraction process of flavonoids from sea buckthorn seed meal of microorganisms and analysis of the antioxidant activity[C]. Beijing: China Association of Fragrance Flavour and Cosmetic Industris, 2016.
[10]	Liu X J, Wang Y H, Li P , et al. Extration of total flavones from marc of sea buckthorn[J]. Journal of Beijing University of Chemical Technology, 2004,31(1):19-21.
[11]	Wang J G, Yao Y J, Liu J M , et al. Study about the adsorption of refined sea buckthorn seed flavonoids by macroporous resin[J]. Seabuckthorn Hippophae, 2007,20(1):16-17.
[12]	Liu X J, Wang Y H, Ma R Y . Refinement of total flavonoids of hippophae with macroporous adsorption resins[J]. China Oils and Fats, 2004,29(5):37-39.
[13]	He Y X . Ultrasonic extraction of flavonoids from Xinjiang jujube and purification of macroporous resin[D]. Tianjin: Tianjin University, 2009.
[14]	Zhang J C, Xie Y F, Yu D . Extraction of flavonoids from rose and flower residue and its antioxidant activity[J]. Science and Technology of Food Industry, 2014,35(22):226-230.
[15]	Tang G E . Technological conditions and antioxidant activities of the flavonoid extracted from rose and rose residue[D]. Yanbian: Yanbian University, 2006.
[16]	Darzynkiewicz Z, Zhao H, Halicka H D , et al. In search of antiaging modalities: evaluation of Mtor and ROS/DNA damage-signaling by cytometry[J]. Cytometry Part A, 2014,85(5):386-399.
[17]	Xiao W S, Li J Y, Yong W S , et al. Research on micro-structure, collagen, matrix metalloproteinase-1 and tissue inhibitors of metalloproteinase-1 in rats’ achilles tendon with enthesiopathy[J]. Chinese Journal of Sports Medicine, 2014.
[18]	Oh E J, Kwon J H, Kim S Y , et al. Red pigment produced by Zooshikella ganghwensis inhibited the growth of human cancer cell lines and MMP-1 gene expression[J]. Applied Biological Chemistry, 2016,59(4):567-571.
[19]	Feng B . Protective effects of plant ectracts on human dermal cells injury induced by H₂O₂[D]. Wuxi: Jiangnan University, 2012.

θ/℃	15	25	35	45	55	–
t/h	0.5	1	1.5	2	2.5	3
φ（洗脱剂）/%	40	50	60	70	80	–

水平	A 料液比/（g∶mL）	B 接菌量/%	C pH
1	1∶60	4	4
2	1∶70	6	5
3	1∶80	8	6

水平	因素				黄酮提取量/（mg·g^-1）
水平	A	空列	B	C	黄酮提取量/（mg·g^-1）
1	1	1	1	1	8.343
2	1	2	2	2	9.376
3	1	3	3	3	8.254
4	2	1	2	3	9.986
5	2	2	3	1	10.976
6	2	3	1	2	11.865
7	3	1	3	2	8.876
8	3	2	1	3	11.976
9	3	3	2	1	11.232
K₁	25.973	29.205	31.184	29.551
K₂	32.827	31.328	29.594	32.117
K₃	32.084	30.351	30.106	29.216
k₁	8.658	9.735	10.395	9.850
k₂	10.942	10.443	9.865	10.706
k₃	10.695	10.117	10.035	9.739
R	2.285	0.708	0.359	0.855

发酵法制备沙棘籽粕总黄酮的初步纯化及抗衰老活性评价

Preliminary purification and anti-aging activity evaluation of total flavonoids prepared from sea-buckthorn seed meal by fermentation

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 14

参考文献 19

相关文章 15

Metrics

本文评价

推荐阅读 10

[1]	李瑶瑶. 异橙黄酮的抗衰老及抗氧化功效研究[J]. 日用化学工业（中英文）, 2024, 54(3): 313-319.
[2]	方婷欢, 郑婷, 蒋晴, 李晓霞, 唐礼荣. 蚕丝丝肽嗜热栖热菌发酵液对皮肤抗炎抗衰功效的增效作用[J]. 日用化学工业（中英文）, 2023, 53(9): 1057-1064.
[3]	郭芳钰, 韩婷婷, 王晓娜, 陈玉荣, 王晓梅, 杨素珍. 中药双向发酵液制备及其抗衰老、保湿、美白功效研究[J]. 日用化学工业（中英文）, 2023, 53(5): 523-531.
[4]	郝晴, 吴二敏, 毕永贤, 刘金俊, 孔德承, 成志伟. 莲子心总黄酮多元醇提取工艺筛选及其性能研究[J]. 日用化学工业（中英文）, 2023, 53(4): 398-407.
[5]	任洁洁. 逆境胁迫处理后山楂叶总黄酮含量变化及对心肌缺血性大鼠分子机制的研究[J]. 日用化学工业（中英文）, 2023, 53(4): 430-436.
[6]	戴丽云, 魏玉洁, 陆震, 王玉玲, 颜欢, 吴越. 糙米发酵滤液—— 一种具有抗衰老潜力的物质[J]. 日用化学工业（中英文）, 2023, 53(3): 292-299.
[7]	马铃, 沈胡驰, 陈殿松, 杨井国, 王靖. 超声辅助酶法提取茶麸黄酮及其对Ⅱ型5α-还原酶抑制活性[J]. 日用化学工业（中英文）, 2023, 53(3): 308-315.
[8]	刘小幸, 陈春宇, 李丽, 易帆. 适应原植物在皮肤健康领域研究现状及展望[J]. 日用化学工业（中英文）, 2023, 53(3): 325-332.
[9]	徐雅妮, 杨凯业, 朱溶滔, 刘光荣, 韩萍, 杜志云. 复合多肽延缓小鼠皮肤衰老的功效研究[J]. 日用化学工业（中英文）, 2023, 53(12): 1421-1428.
[10]	王玥, 李俊, 顾仕红, 王曼, 邹岳, 吴建铭. 一种酿酒酵母和副干酪乳杆菌发酵产物滤液复合物对面部微生态的影响[J]. 日用化学工业（中英文）, 2023, 53(11): 1241-1249.
[11]	牛文霞,何先喆,韩兴艳. 一款含死海水乳液的抗衰功效评价研究[J]. 日用化学工业, 2022, 52(8): 851-857.
[12]	刘丰,罗俊,王晓炜,应国红. 含发酵产物类化妆品微生物检查方法适用性研究[J]. 日用化学工业, 2022, 52(8): 839-843.
[13]	李海军,马双双,郑德强,张英华,王庆波,薛泽. 小球藻发酵产物抗衰老功效研究及安全性评价[J]. 日用化学工业, 2022, 52(5): 534-538.
[14]	王冬冬,孙倩茹,王子文,方嘉璇,李萌,王昌涛. 葡萄籽发酵液抗衰老及美白功效研究[J]. 日用化学工业, 2022, 52(5): 545-552.
[15]	张玉芝,赵丹,王冬冬,张佳婵,王昌涛,李萌. 枸杞枝发酵液中黄酮类成分的活性研究[J]. 日用化学工业, 2022, 52(4): 390-395.