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日用化学工业（中英文）

日用化学工业 ›› 2019, Vol. 49 ›› Issue (10): 668-673.doi: 10.3969/j.issn.1001-1803.2019.10.007

• 开发与应用 • 上一篇    下一篇

利用HDF和HUVEC细胞模型评价4种蜂毒体外抗衰老及其抗炎的功效研究

刘印康1,2,张根生1(),黄国忠3,陈济宽3,张红城1,2   

  1. 1. 哈尔滨商业大学 食品工程学院,黑龙江 哈尔滨 150076
    2. 中国农业科学院 蜜蜂研究所,北京 100093
    3. 龙陵县黄氏蜂业有限公司,云南 保山 678300
  • 收稿日期:2019-01-29 修回日期:2019-09-02 出版日期:2019-10-22 发布日期:2019-10-24
  • 通讯作者: 张根生
  • 作者简介:刘印康（1993-）,男,河北人,硕士,电话：18303013479,E-mail： 994640253@qq.com。
  • 基金资助:
    云南省重大科技专项计划(2018ZF01204)

Evaluation of anti-aging and anti-inflammatory effects of four beevenoms in vitro by HDF and HUVEC cell models

LIU Yin-kang1,2,ZHANG Gen-sheng1(),HUANG Guo-zhong3,CHEN Ji-kuan3,ZHANG Hong-cheng1,2   

  1. 1. College of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150076, China
    2. Institute of Agricultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China
    3. Huangshi Bee Industry Co., Ltd., Baoshan, Yunnan 678300, China
  • Received:2019-01-29 Revised:2019-09-02 Online:2019-10-22 Published:2019-10-24
  • Contact: Gen-sheng ZHANG

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摘要：

为探究金黄虎头蜂、葫芦蜂、夜食蜂和意蜂蜂毒体外抗衰老的功效,检测了4种蜂毒对人真皮成纤维细胞（HDF）模型分泌的I型胶原蛋白（Col-I）和基质金属蛋白酶（MMP-1）的影响;为探究金黄虎头蜂、葫芦蜂、夜食蜂和意蜂蜂毒体外抗炎功效,检测了4种蜂毒对脂多糖（LPS） 诱导的人脐带静脉内皮细胞（HUVEC）炎症模型分泌的炎症因子一氧化氮（NO）、人白细胞介素6（IL-6）和人白细胞介素8（IL-8）的影响。结果表明,当金黄虎头蜂和葫芦蜂蜂毒质量浓度为10 mg/L时,HDF细胞分泌的MMP-1的含量分别降低了26.03%和43.64%,而Col-I的含量分别增加了24.62%和30.31%;4种蜂毒刺激HUVEC细胞后,均能降低细胞分泌的炎症因子的含量,意蜂蜂毒质量浓度为5 mg/L时,IL-6的含量与模型组相比降低了62%;意蜂蜂毒质量浓度为10 mg/L时,其抑制细胞分泌IL-8的作用效果最明显,降低了模型组IL-8含量的68%。4种蜂毒均具有抗炎功效,金黄虎头蜂和葫芦蜂蜂毒具有抗衰老功效。

关键词: 蜂毒, 体外, 抗衰老, 抗炎

Abstract:

To investigate the anti-aging effects of Vespa mandarinia Smith, Vespa bicolor Fabricius, Xylocopa（Nyctomelitta） tranquebarica and Apis mellifera venom in vitro, type I collagen（Col-I） and matrix metalloproteinase（MMP-1） secreted by human dermal fibroblasts（HDF） was detected. And in order to explore the anti-inflammatory effects of Vespa mandarinia Smith, Vespa bicolor Fabricius, Xylocopa（Nyctomelitta） tranquebarica and Apis mellifera venom in vitro, the inflammatory factors nitric oxide（NO）, human interleukin-6（IL-6） and human interleukin 8（IL-8） secreted by lipopolysaccharide（LPS）-induced human umbilical vein endothelial cells（HUVEC） were detected. The results show that when the concentration of Vespa mandarinia Smith and Vespa bicolor Fabricius venom is 10 mg/L, the contents of MMP-1 secreted by HDF cells decrease by 26.03% and 43.64%, while the contents of Col-I increase by 24.62% and 30.31%, respectively. After stimulation of HUVEC cells by four bee venoms, the contents of inflammatory cytokines secreted by cells are reduced. At 5 mg/L, the content of IL-6 is reduced by 62% compared with the model group. When the concentration of bee venom is 10 mg/L, its inhibitory effect on IL-8 secretion is the most obvious, which reduces the content of IL-8 by 68% in the model group. In summary, four bee venoms have anti-inflammatory effects, but only Vespa mandarinia Smith and Vespa bicolor Fabricius venom have anti-aging effects.

Key words: bee venom, in vitro, anti-aging, anti-inflammatory

中图分类号: 

  • TQ658