三七不同提取物舒缓功效对比研究

doi:10.3969/j.issn.2097-2806.2026.08.010

摘要/Abstract

摘要：

使用体外生物学实验方法对三七不同提取物舒缓功效及其功效差异进行研究。采用红细胞溶血实验、透明质酸酶抑制实验，检测细胞溶血率及透明质酸酶抑制率，评价其对细胞保护作用及抗过敏活性；采用UVB诱导原代人角质形成细胞（KC）炎症模型，检测上清中IL-6、TNF-α的含量，评价其抗炎活性；采用十二烷基硫酸钠（SLS）诱导KC细胞屏障损伤模型，检测KC细胞活力、迁移率及ZO-1、TGM1、Claudin-1蛋白的表达，评价其屏障修复功效；采用辣椒素诱导的KC细胞模型，检测KC细胞中Ca²⁺荧光强度及TRPV1、TRPM2蛋白的表达，评价其改善神经过敏性反应的功效。结果显示，三七不同提取物均能显著抑制红细胞溶血率及透明质酸酶的活性，降低炎症细胞中IL-6、TNF-α炎症因子的含量，抑制细胞中Ca²⁺的内流；三七不同提取物能显著提高经UVB照射后KC细胞的存活率，促进经SLS损伤后的KC细胞迁移及相关屏障修复相关蛋白ZO-1、Claudin-1的表达，能显著抑制经辣椒素诱导后TRPV1、TRPM2蛋白的表达。表明三七不同提取物有较好的舒缓功效，且三七总皂苷舒缓功效优于三七总黄酮、三七总多糖。

关键词: 舒缓功效评价, 三七提取物, 炎症, 屏障修复, 神经反应

Abstract:

In this study, we investigated the effects of different extracts of Panax notoginseng （PNT） on cell protection, barrier repair, and the alleviation of inflammatory response and neurological response, and evaluated the soothing efficacy of these extracts. The results show that different extracts of PNT significantly inhibit the red blood cell hemolysis rate and hyaluronidase activity, reduce the levels of the inflammatory cytokines IL-6 and TNF-α in stimulated cells, and inhibit intracellular Ca²⁺ influx. Furthermore, total saponins of PNT and total polysaccharides of PNT significantly increase the survival rate of KC cells after UVB irradiation, promote KC migration following SLS-induced injury, upregulate the expression of key barrier repair proteins （ZO-1 and claudin-1）, and significantly inhibit the capsaicin-induced expression of TRPV1 and TRPM2 proteins. In conclusion, various PNT extracts exhibit strong soothing properties, with PNT saponins demonstrating superior soothing efficacy compared to PNT flavonoids and PNT polysaccharides.

Key words: soothing efficacy evaluation, Panax notoginseng extract, inflammation, skin barrier repair, neurogenic response

中图分类号:

TQ658

何志明, 黄加文, 詹易, 吴德松, 查雨锋. 三七不同提取物舒缓功效对比研究[J]. 日用化学工业（中英文）, 2026, 56(8): 1053-1061.

Zhiming He, Jiawen Huang, Yi Zhan, Desong Wu, Yufeng Zha. Comparative study on the soothing efficacy of different extracts of Panax notoginseng[J]. China Surfactant Detergent & Cosmetics, 2026, 56(8): 1053-1061.

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