三白草提取物的抗皱和紧致功效研究

doi:10.3969/j.issn.2097-2806.2026.08.009

摘要/Abstract

摘要：

文章旨在通过分子对接、细胞学验证、人体皮肤功效验证来探究三白草提取物的抗皱和紧致功效。分子对接结果显示，三白草提取物中的主要成分三白草酮与内皮素A受体（EDNRA）结合力能达-6.619 kcal/mol，结合活性良好。内皮素-1 （ET-1）刺激过表达EDNRA的HaCaT细胞结果显示，三白草提取物可显著降低EDNRA激活后升高的细胞内钙离子浓度，降低基质金属蛋白酶-9 （MMP-9）表达量。人体单盲半脸对照试验结果显示，与基质组相比，三白草提取物在使用后7天、14天、28天均显著提高皮肤高密度回声值，在28天显著降低鱼尾纹最大深度、皮肤回弹时间及皮肤红度a^*值、皮肤粗糙度参数R_a和R_z。三白草提取物可能通过调控ET-1-EDNRA通路，影响细胞因子MMP-9表达量来发挥抗皱和紧致功效。

关键词: 三白草提取物, EDNRA, MMP-9, 抗皱功效, 紧致功效

Abstract:

This study aimed to investigate the anti-wrinkle and firming efficacy of Saururus chinensis extract （SC） using molecular docking, cell-based assays, and a single-blind, half-face controlled human trial. The results show that sauchinone, a characteristic bioactive component of SC, exhibits strong binding affinity for EDNRA, with a binding energy of -6.619 kcal/mol. In EDNRA-overexpressing HaCaT cells, SC significantly reduces the elevation of intracellular Ca²⁺ level after EDNRA activation and decreases the expression level of MMP-9. In the half-face controlled clinical trial, SC significantly increases the ultrasound high-intensity echogenicity of the skin at 7, 14, and 28 days compared with the vehicle control. By Day 28, SC treatment significantly reduced crow’s feet depth, skin retraction time, skin redness a^* value, and skin roughness （R_a and R_z values）. Therefore, SC has anti-wrinkle and firming efficacy by modulating MMP-9 expression through the ET-1/EDNRA signaling pathway.

Key words: Saururus chinensis extract, EDNRA, MMP-9, anti-wrinkle efficacy, firming efficacy

中图分类号:

TQ658

周菲, 刘宗艳, 喻明英, 张力川, 郭静怡, 卢伊娜. 三白草提取物的抗皱和紧致功效研究[J]. 日用化学工业（中英文）, 2026, 56(8): 1044-1052.

Fei Zhou, Zongyan Liu, Mingying Yu, Lichuan Zhang, Jingyi Guo, Yina Lu. Study on the anti-aging and firming efficacy of Saururus chinensis extract[J]. China Surfactant Detergent & Cosmetics, 2026, 56(8): 1044-1052.

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相	中文名称	w/%
相	中文名称	基质	0.5% SC	2% SC
A	甘油硬脂酸酯、PEG-100硬脂酸酯	2	2	2
	鲸蜡硬脂醇	5	5	5
	凡士林	3	3	3
	矿油	10	10	10
	水	69.2	70.7	69.2
B	甘油	6	6	6
	1, 3-丙二醇	2	2	2
	对羟基苯乙酮	0.4	0.4	0.4
	1, 2-己二醇	0.4	0.4	0.4
C	辛酸/癸酸甘油三酯、三白草（Saururus chinensis）提取物	0	0.5	2
C	辛酸/癸酸甘油三酯	2	0	0