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日用化学工业（中英文）

日用化学工业（中英文） ›› 2025, Vol. 55 ›› Issue (9): 1187-1197.doi: 10.3969/j.issn.2097-2806.2025.09.013

• 开发与应用 • 上一篇    下一篇

粕类蛋白发酵氨基酸/肽的活性及其护肤功效

谢思跃1,秦灿2,林俊敏1,罗晓春2,*()   

  1. 1.广东省轻工业技师学院 轻化工程学院，广东 广州 511330
    2.华南理工大学 生物科学与工程学院，广东 广州 510006
  • 收稿日期:2024-09-29 修回日期:2025-09-01 出版日期:2025-09-22 发布日期:2025-10-11
  • 基金资助:
    广东省乡村振兴战略专项资金种业振兴行动项目(2022-WPY-00-11);广州市科技计划项目(202206010137);广州省自然科学基金项目(2022A1515010568)

The activity and skin-caring efficacy of amino acids/peptides fermented from meal protein

Siyue Xie1,Can Qin2,Junmin Lin1,Xiaochun Luo2,*()   

  1. 1. School of Light Chemical Engineering, Guangdong Technician College of Light Industry, Guangzhou, Guangdong 511330, China
    2. School of Biological Sciences and Engineering, South China University of Technology, Guangzhou, Guangdong 510006, China
  • Received:2024-09-29 Revised:2025-09-01 Online:2025-09-22 Published:2025-10-11
  • Contact: *E-mail: xcluo@scut.edu.cn.

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摘要： 以链霉菌SCUT-3-3940为菌株进行固态发酵，得到豆粕、棉籽粕和菜籽粕三种粕类植物蛋白发酵后的氨基酸/肽（SAP、CAP、RAP），通过体外法与在体法系统地比较研究其生物活性和护肤功效。结果显示：三批试样稳定性好，不具有刺激性，小鼠皮肤对SAP中的氨基酸和多肽有很好的吸收效果。三种氨基酸/肽（SAP、CAP、RAP，添加量均为480 µg/mL）在全血清添加培养基中的HSF细胞生长有显著促进作用，细胞生长率分别提高了53.4%，30.1%，30.9%；同剂量添加，HSF细胞的划痕修复面积在24 h比对照组分别提高了98.4%，99.0%，54.7%；三种原料能有效减少紫外损伤造成的ROS产生，对光老化细胞有保护作用。人体试验表明，在640 µg/mL添加量下，受试者使用样品组2 h后的皮肤角质层含水量（MMV）显著高于基质空白组（P<0.05），其中SAP组的MMV值增加了21.58%；通过低敏胶带进行皮肤受损造模，受试者使用同添加量的精华乳3 h后的皮肤颜色a值和皮肤经皮水分流失量（TEWL）均明显低于基质空白组，其中SAP组受试者的a值降低了21.46%，TEWL值减少40.84%。研究表明，SAP、CAP、RAP具有多种生物活性和良好的保湿、抗光老化和皮肤屏障修复等护肤功效，为粕类副产物实现高值化利用提供新的方向和理论参考。

关键词: 粕类植物蛋白, 固态发酵, 氨基酸/肽, 生物活性, 护肤, 修复

Abstract:

This study examines the bioactivity and skincare efficacy of amino acids and peptides derived from the solid-state fermentation of soybean meal, cottonseed meal, and rapeseed meal by Streptomyces sp. SCUT-3-3940, referred to as SAP, CAP, and RAP, respectively. In vitro and in vivo methods were employed to comprehensively compare their effects. The results indicate that all three samples exhibit notable stability and are non-irritating, with SAP demonstrating excellent absorption of amino acids and peptides in mouse skin. At a concentration of 480 µg/mL, all three amino acid and peptide samples significantly enhance the growth of HSF cells in serum-supplemented media, increasing the growth rate by 53.4%, 30.1%, and 30.9%, respectively. Furthermore, at the same concentration, the scratch wound healing of HSF cells is enhanced by 98.4%, 99.0%, and 54.7% after 24 hours compared to the control group. The samples also effectively reduce ROS production induced by UV damage, thereby providing protection against photoaging. Human trials reveal that at a concentration of 640 µg/mL, the stratum corneum moisture content （MMV） in the sample group significantly increases 2 hours post-application compared to the blank group （P<0.05）, with SAP enhancing MMV by 21.58%. Moreover, in a skin damage model induced by low-sensitivity tape, skin redness （a value） and transepidermal water loss （TEWL） in the sample group are significantly lower 3 hours post-essence application compared to the blank group, with SAP reducing redness by 21.46% and TEWL by 40.84%. These findings indicate that SAP, CAP, and RAP exhibit diverse bioactivities, including moisturizing, anti-photoaging, and skin barrier repair properties, offering new insights and theoretical frameworks for the high-value utilization of plant meal by-products.

Key words: meal type plant protein, solid state fermentation, amino acids and peptides, biological activity, skincare, skin barrier repair

中图分类号: 

  • TQ658