欢迎访问《日用化学工业（中英文）》，今天是
日用化学工业（中英文）

日用化学工业 ›› 2017, Vol. 47 ›› Issue (6): 317-321.doi: 10.13218/j.cnki.csdc.2017.06.004

• 开发与应用 • 上一篇    下一篇

茯苓提取物对酪氨酸酶抑制动力学及刺激性研究

陈宇霞1, 张凯1, 龚盛昭1,2   

  1. 1.广州环亚化妆品科技有限公司,广东 广州 510663;
    2.广东轻工职业技术学院 精细化工研发中心,广东 广州 510300
  • 收稿日期:2016-12-21 发布日期:2019-03-20
  • 通讯作者: 龚盛昭,教授,博士,电话:(020)32098888,E-mail:328415887@qq.com。
  • 作者简介:陈宇霞(1982-),女,广东人,助理工程师,电话:(020)32098888,E-mail:357582159@qq.com。
  • 基金资助:
    广东省高等职业院校珠江学者岗位计划资助项目(2013);广东省高等学校高层次人才项目(2013QY02); 2017年广州市科技计划项目产学研协同创新重大专项(201604016111)

Tyrosinase activity inhibition kinetics and skin irritation of Poria cocos extract

CHEN Yu-xia1, ZHANG Kai1, GONG Sheng-zhao1,2   

  1. 1.Guangzhou Huanya Cosmetics Technology Co.,Ltd.,Guangzhou,Guangdong 510663,China;
    2.Research Centre of Fine Chemical Technique,Guangdong Industry Technical College,Guangzhou,Guangdong 510300,China
  • Received:2016-12-21 Published:2019-03-20

PDF (PC)

428

赞

摘要： 研究了茯苓提取物对酪氨酸酶单酚酶和二酚酶活力的影响及其抑制动力学,测试结果表明:茯苓提取物对酪氨酸酶单酚酶和二酚酶的活力均有抑制作用,单酚酶和二酚酶活力下降50%的茯苓提取物质量浓度(IC50)分别为0.008和0.040 g/L。酪氨酸酶单酚酶的催化反应存在迟滞时间,茯苓提取物对酪氨酸酶二酚酶活力的抑制作用表现为可逆效应。Lineweaver-Burk图显示茯苓提取物对二酚酶的作用表现为非竞争性与竞争性混合的抑制类型。在此基础上采用红细胞溶血实验初步评估茯苓提取物对皮肤的刺激性,结果显示含2.000 g/L茯苓提取物的样品无刺激性,质量浓度远大于单酚酶和二酚酶活力下降50%的茯苓提取物质量浓度IC50,茯苓提取物在0.200~0.800 g/L的质量浓度范围内可保证其安全添加且具有美白功效。

关键词: 美白化妆品添加剂, 茯苓提取物, 酪氨酸酶, 混合型抑制, 刺激性

Abstract: The kinetics of inhibitory effect of Poria cocos extract on monophenolase and diphenolase activity of mushroom tyrosinase was studied.Test results show that Poria cocos extract efficiently inhibits both monophenolase and diphenolase activities of mushroom tyrosinase.Concentrations of Poria cocos extract leading to 50% activity lost (IC50) on monophenolase and diphenolase were calculated to be 0.008 and 0.040 g/L respectively.There is a lag time in the catalytic reaction process of the monophenolase.Inhibition of Poria cocos extract on activity of diphenolase of tyrosinase indicates a reversible effect.Lineweaver-Burk plots indicates that the inhibitor binds the enzyme reversibly other than reacts with it destructively.Lineweaver-Burk plots show that the inhibition type is a composite of competitive and non-competitive inhibition.On this basis,Poria cocos extract stimulus preliminary assessment was conducted with RBC hemolysis test,and results show that Poria cocos extract with a mass concentration of 2.000 g/L is non-stimulus while such concentration is far higher than IC50 of Poria cocos extract on monophenolase and diphenolase activity.It further indicates that Poria cocos extract displays whitening effect under the condition of the security.This suggests that Poria cocos extract with mass concentration range of 0.200~0.800 g/L is safe,for whitening cosmetic use.

Key words: additive of whitening cosmetics, Poria cocos extract, tyrosinase, mixed inhibition, stimulus

中图分类号: 

  • TQ658