四物汤美白功效评价及其药效关系研究

doi:10.13218/j.cnki.csdc.2015.01.011

摘要/Abstract

摘要： 利用水提法提取四物汤,采用DPPH(1,1-二苯基-2-三硝基苯肼)分析法测定四物汤的抗氧化活性,用紫外分光光度法测定四物汤对酪氨酸酶的抑制作用,综合评价四物汤的美白功效。同时以对DPPH自由基的清除率和对酪氨酸酶的抑制率为评价指标,运用均匀设计法研究四物汤剂量比例与美白功效的关系。结果表明,用水提法提取的四物汤具有较强清除DPPH自由基的能力,对酪氨酸酶活性呈高质量浓度抑制,低质量浓度激活的作用。均匀设计实验中,当四物汤采用m(当归)∶m(川芎)∶m(白芍)∶m(熟地)=85∶66∶10∶199时,对酪氨酸酶活性的抑制率为24.3%,对DPPH自由基的清除率为95.2%,具有较好的美白效果。

关键词: 美白, 四物汤, 1,1-二苯基-2-三硝基苯肼, 酪氨酸酶, 均匀设计

Abstract: Siwu-tang was prepared by water extraction of four kinds of Chinese traditional herb medicine.DPPH (1,1-diphenyl-2-picryl-hydrazyl) method was adopted to measure the antioxidant activity of the Siwu-tang and ultraviolet spectrophotometry was adopted to measure the tyrosinase inhibitory activity.Then the whitening efficacy of Siwu-tang was comprehensively evaluated.Meanwhile,taking DPPH free radical scavenging ratio and tyrosinase inhibitory ratio as assessment indices,as well as adopting uniform design method,the relationship between dosage ratio of the Siwu-tang and the whitening efficacy was investigated.Results showed that,the Siwu-tang displays rather high DPPH free radical scavenging capacity.With respect to activity of tyrosinase,it displays inhibitory action at high mass concentration,while at lower mass concentration it displays activation effect.In the uniform designed experiment,the results showed that when the mass ratio in the composition of the Siwu-tang Radix Angelica sinensis,Rhizoma Chuanxiong,Radix Paeoniae Alba and Radix Rehmanniae Praeparata is 85∶66∶10∶199,the inhibitory ratio of tyrosinase activity achieves 24.3%,and the scavenging ratio of DPPH free radicals achieves 95.2%.

Key words: whitening, Siwu-tang, 1,1-diphenyl-2-picryl-hydrazyl, tyrosinase, uniform design

中图分类号:

TQ658.5

尤卓莹, 何秋星, 李馨恩, 黄永红, 林春雨. 四物汤美白功效评价及其药效关系研究[J]. 日用化学工业, 2015, 45(1): 45-48.

YOU Zhuo-ying, HE Qiu-xing, LI Xin-en, HUANG Yong-hong, LIN Chun-yu. Study on the whitening efficacy of Siwu-tang and the relationshipwith its pharmacodynamic function[J]. China Surfactant Detergent & Cosmetics, 2015, 45(1): 45-48.

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