Table of Content

22 March 2023, Volume 53 Issue 3 Previous Issue    Next Issue

Basic research

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Study on Pickering emulsion stabilized by hydroxyethyl cellulose/nanocellulose and its rheological properties Ding Zhengqing, Wu Yingyi, Wang Weiyun, Huang Xujuan, Cai Zhaosheng 2023, 53 (3):  245-252.  doi: 10.3969/j.issn.1001-1803.2023.03.001 Abstract ( 433 )   HTML ( 43 )   PDF (4471KB) ( 341 )   Nanocellulose （CNC） was prepared from α-Cellulose by acid hydrolysis method, and its structure and micromorphology were characterized. Pickering emulsion was prepared by using soybean oil as the oil phase and hydroxyethyl cellulose （HEC） and CNC as mixed emulsifiers （HEC/CNC）. The emulsion type was determined to be W/Oemulsion by dilution method. The thermal stability of the Pickering emulsion was tested, and the results showed that the stability of the Pickering emulsion stabilized by HEC/CNC was stronger than that of the Pickering emulsion prepared by CNC. The effects of CNC mass fraction, oil-water ratio, and HEC mass fraction on the stability of Pickering emulsion were studied. The rheological properties of Pickering emulsion stabilized by HEC/CNC were analyzed. The results showed that, with the increase of HEC mass fraction, the stability of Pickering emulsion was enhanced. When the oil-water ratio was 8∶2, the CNC mass fraction was 0.2% and the HEC mass fraction was 0.4%, the Pickering emulsion exhibited the strongest stability, and its emulsion droplet size was approximately 20 μm. The Pickering emulsion stabilized by HEC/CNC thus prepared could be stable for 100 days. The Pickering emulsion stabilized by HEC/CNC had stronger stability and smaller droplet diameter than that of the CNC-stabilized Pickering emulsion. The Pickering emulsion stabilized by HEC/CNC exhibited a rheological property of “shear-thinning”. When the HEC mass fraction in solution was increased, the shear viscosity of the emulsion decreased with the increase of shear rate. Figures and Tables | References | Related Articles | Metrics

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Effects of Mg2+, Al3+ and Fe3+ on the surface properties of fluorapatite Jiang Chunyan, Ao Xianquan, Cao Yang, Chen Hong, Li Songhong 2023, 53 (3):  253-259.  doi: 10.3969/j.issn.1001-1803.2023.03.002 Abstract ( 147 )   HTML ( 16 )   PDF (1351KB) ( 720 )   Phosphate rock flotation is a widely used method in phosphate rock processing in which aimed minerals are selectively separated from gangue minerals based on the difference in surface wettability. Due to its associated minerals in phosphate rock, there is always a lot of Mg2+, Al3+ and Fe3+ in phosphate rock slurry. The existence of metal ions, whose forms are changed with slurry pH, can affect the flotation operation by adsorption on the mineral surface. The surface properties of minerals are the main factors affecting their dispersion, aggregation, and flotation behavior. Herein, the influences of unavoidable metal ions, including Mg2+, Al3+ and Fe3+, on the surface properties of fluorapatite （FAP） in pulp were studied by solution chemical calculation, turbidity, contact angle and Zeta-potential test. The results showed that the forms of metal ions in solution vary with pH. FAP is a hydrophilic mineral, and the influence of adding Fe3+ ions on the wettability of FAP was greatly affected by pH. In acidic environment, Fe3+ ions could promote the hydrophilicity of FAP, while in neutral and alkaline environment, Fe3+ ions could inhibit the hydrophilicity of FAP. The effects of Mg2+ and Al3+ ions were less affected by pH, and both Mg2+ and Al3+ ions inhibited the wettability of FAP in neutral environment. In the range of pH 3-11, Mg2+ mainly existed as divalent ions, which compressed the double layer on the FAP surface. Al3+ and Fe3+ mainly generated hydroxide precipitation through hydrolysis to cover the surface of FAP. Mg2+, Al3+ and Fe3+ ions would destroy the dispersity of FAP and promote the coagulation of FAP. Figures and Tables | References | Related Articles | Metrics

Lecture of science and technology

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Scientific foundations of hair and scalp care （Ⅲ）Mechanical properties of human hair Wu Yuwen, Bian Marina, Yue Zoe, Chang Kuan, Wang Jing 2023, 53 (3):  260-270.  doi: 10.3969/j.issn.1001-1803.2023.03.003 Abstract ( 445 )   HTML ( 25 )   PDF (2916KB) ( 582 )   The strength of mechanical properties is one of the most important criteria to measure the health conditioner of the hair. In healthy hair bundles, hair fiber processes excellent mechanical properties, while damaged hair bundles can break during daily combing. However, hair fiber is not a homogeneous material, so it is complicated to characterize its mechanical properties systematically and scientifically. Based on this point, this paper summarizes the testing methods of hair mechanical properties and the meaning of tensile curves, describes the influence of hair internal structure and composition on tensile properties, lists several factors affecting hair tensile properties and introduces several commercially available ingredients to enhance hair tensile properties and their mechanisms of action. This review is expected to provide a reference for the development of new hair care actives and products with hair strengthening effect. Figures and Tables | References | Related Articles | Metrics

Development and application

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Synthesis and washing aid properties of a polycarboxylate copolymer Gao Dangge, Li Pengyu, Yu Shuzhen, Liu Wei, Lv Bin 2023, 53 (3):  271-278.  doi: 10.3969/j.issn.1001-1803.2023.03.004 Abstract ( 170 )   HTML ( 18 )   PDF (1463KB) ( 141 )   Poly（ESA-AA-SAS-HPA） was synthesized by solution polymerization from epoxy succinic acid （ESA）, acrylic acid （AA）, sodium allyl sulfonate （SAS） and hydroxypropyl acrylate （HPA）. It was characterized by FT-IR,1H NMR, TG and DLS. The effects of chelating pH, temperature and additive mass fraction on the washing aid performance of Poly（ESA-AA-SAS-HPA） were investigated. The results show that, compared with sodium tripolyphosphate, it has good washing aid performance, with the chelating amount of 308.7 mg/g and the calcium dispersing power of 101.5 mg/g. Poly（ESA-AA-SAS-HPA） has good synergism with sodium dodecylbenzene sulfonate （LAS）, which can reduce the surface tension of the system to 45 mN/m. Figures and Tables | References | Related Articles | Metrics

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Preparation and performance evaluation of organic zirconium gelled fracturing fluid with high temperature resistance and slowly-cross-linking Jin Zhen, Zhang Chunsheng 2023, 53 (3):  279-284.  doi: 10.3969/j.issn.1001-1803.2023.03.005 Abstract ( 154 )   HTML ( 6 )   PDF (1218KB) ( 174 )   To solve the problems of poor temperature resistance, short cross-linking time and high residue content of fracturing fluids commonly used in field, an organic zirconium cross-linking agent YJ-3 was successfully synthesized from zirconium oxychloride, polyols, organic ligands, complex cross-linking agent and high temperature stabilizer. On this basis, a temperature resistant and slowly-cross-linking organic zirconium gelled fracturing fluid was prepared by selecting polyacrylamide thickener RG-1 and additives. Mass fractions of YJ-3 and RG-1 were optimized by single factor method, and the performance indexes of the fracturing fluid system were evaluated. Experimental results showed that the optimal composition of the fracturing fluid was 0.50% RG-1+2.0% YJ-3+0.15% Na2S2O3+0.8% cleanup additive. The cross-linking time was adjustable between 3 and 45 min. The viscosity was still higher than 50 mPa·s at 90 ℃ and 150 ℃ after shear at 170 s-1 for 2 h. The sand-carrying performance and filtration property were obviously better than that of conventional fracturing fluid system based on guar gum. Fracturing fluid could be completely broken within 1-3 h, without residue and easy to flow back. It is a kind of temperature-resistant, slowly-cross-linking and low-damage fracturing fluid with excellent performance, which has a broad application prospect. Figures and Tables | References | Related Articles | Metrics

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Cloning and expression of a novel gene encoding agarase from Microbulbifer sp. FG4 Zhang Lixiong, Liu Mingming, Chen Liang, Fang Zaiguang 2023, 53 (3):  285-291.  doi: 10.3969/j.issn.1001-1803.2023.03.006 Abstract ( 135 )   HTML ( 6 )   PDF (1731KB) ( 170 )   Based on the flanking sequence of the agarase gene of FG4 strain, the recombinant plasmid and vector were constructed to obtain the engineering strain which could express the agarase efficiently. In this study, the flanking sequence of FG4 agarase gene is obtained by chromosome walking. The cloned gene is recombined with the expression vector pET-22b （+）and transferred into the recipient BL21. After the successful construction of the expressed strain, the expression of agarase is induced by IPTG and the optimal fermentation conditions of the expressed strain are explored. This strain ensures the catalytic activity of agarase and lays the foundation for the agarase-based pharmaceutical industry, daily chemical industry and food industry. The cloning and expression of agarase gene from Microbulbifers sp. FG4 strain of Microbulbifers provides theoretical support for agarase application in different fields. Figures and Tables | References | Related Articles | Metrics

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Fermented brown rice filtrate: a substance with anti-aging potential Dai Liyun, Wei Yujie, Lu Zhen, Wang Yuling, Yan Huan, Wu Yue 2023, 53 (3):  292-299.  doi: 10.3969/j.issn.1001-1803.2023.03.007 Abstract ( 449 )   HTML ( 20 )   PDF (3075KB) ( 246 )   In recent years, brown rice has gained growing attention due to its obvious advantage of being rich in bioactive compounds. The objective of this study was to evaluate the anti-aging potential of fermented brown rice filtrate （FBR） by Saccharomyces cerevisiae for cosmetics. Results for the FBR were compared to the brown rice aqueous solution （BR） that was not fermented. In this study, the total matter content of brown rice is almost unchanged before and after fermentation, but the small molecular matter increases obviously. In a DPPH free radical scavenging test, the antioxidant activity is greater in FRG compared to RG. In a human epidermal keratinocyte （HaCaT） proliferation test, FBR with a volume fraction of 0.2% and 1% shows the high proliferative capacity, while the BR with a volume fraction of 1% is characterized by high cytotoxicity to HaCaT. Meanwhile FBR shows anti-aging activity by increasing the expression of autophagy marker LC3B and Collagen I after AGEs-treated, while decreasing intracellular ROS production. Further study shows that FBR can promote the production of hyaluronic acid （HA） and the expression of its receptor CD44 in HaCaT, and enhance the expression of collagen I in human primary fibroblasts （FB）. Taken together, these data indicates that FBR has the potential of anti-aging may be applied to cosmetics products. Figures and Tables | References | Related Articles | Metrics

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Study on skin care effects of Rosa graciliflora callus extract in human dermal fibroblast cells and 3D epidermal model Li Huiling, Zhou Chunxia, Zhang Zhang 2023, 53 (3):  300-307.  doi: 10.3969/j.issn.1001-1803.2023.03.008 Abstract ( 180 )   HTML ( 16 )   PDF (1511KB) ( 265 )   The Rosa graciliflora callus was induced from seedling leaves by plant tissue culture, and the Rosa graciliflora callus extract （RCE） was obtained by ultrasonic extraction and lyophilization. To investigate the active component of RCE and its skin care efficacy in vitro, the contents of amino acids, vitamins and organic acids were detected by LC-MS, and the effect of RCE on skin was evaluated by normal human dermal fibroblast （HDF） cells and 3D epidermal model. Cells viabilities and the content of collagen I （Col-I） were detected in HDF cells to evaluate the skin anti-aging function and the immunohistochemical staining （Ki67） and immunofluorescence staining （Filaggrin and Involucrin） were detected in 3D epidermal model to evaluate the skin barrier function of RCE. The results show that 4.27%±0.23% RCE powder can be obtained by ultrasonic extraction and lyophilization. In addition, LC-MS results show that RCE contained 3.88% amino acids, 0.003 5% vitamins, and 1.04% organic acids, and other experimental results show that RCE also contained 6.20% minerals, 43.80% sugar, 10.00% protein, 1.37% polyphenols, 14.6% ash and 21.90% water. Furthermore, cells assay results show that RCE can significantly promote HDF cells viability and type I collagen content. Compared with the blank control, 0.01% and 0.05% RCE can increase type I collagen content by 83% and 79%, respectively （P<0.01）, with the effect similar to the positive control vitamin C. Moreover, in the reconstructed 3D epidermal model assay, compared with the results of the model group, 0.01% RCE can significantly up-regulate the expression of Ki67, Filaggrin and Involucrin, the relative positive rates of Ki67, Filaggrin and Involucrin are increased by 308%, 853% and 349%, respectively, and the fluorescence intensities of Filaggrin and Involucrin are increased by 824% and 159%, respectively. The results indicate that RCE has the potential effect of anti-aging and improving skin barrier function and is expected to be used as a green raw material in cosmetics. Figures and Tables | References | Related Articles | Metrics

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Ultrasound-assisted enzymatic hydrolysis of flavonoids from camellia seed cake and the inhibitory activity on 5α-reductase type Ⅱ Ma Ling, Shen Huchi, Chen Diansong, Yang Jingguo, Wang Jing 2023, 53 (3):  308-315.  doi: 10.3969/j.issn.1001-1803.2023.03.009 Abstract ( 159 )   HTML ( 5 )   PDF (1927KB) ( 245 )   The process conditions of ultrasonic-assisted enzymatic hydrolysis of flavonoids from camellia seed cake were optimized by orthogonal tests, and the enzymatic reaction system of 5α-reductase type Ⅱ in vitro was established to investigate the activity of camellia seed cake extract on 5α-reductase type Ⅱ. Moreover, the molecular docking model was used to simulate and predict the inhibition of 5α-reductase type Ⅱ by flavonoids from camellia seed cake, which provided the basis for the development of camellia seed cake and other plants. The results show that the extraction process optimized by the orthogonal experiment is as follows: 50 ℃, 50% ethanol, adjusting pH to 6 for enzymatic hydrolysis, adding 2.5% of camellia seed cake for enzymatic hydrolysis for 1 h, and then ultrasonically extracting at 45 kHz, 50 ℃ for 30 min. The yield of total flavonoids is （10.13±0.32） mg/g under this optimized condition. It is preliminarily deduced that camellia seed cake mainly contains kaempferol 3-O-[2-O-β-D-galactose-6-O-α-L-rhamnose]-β-D-glucoside and kaempferol 3-O-[2-O-β-D-xylose-6-O-α-L-rhamnose]-β-D-glucoside by chromatography coupled with mass spectrometry （UPLC Q-TOF MS）. Camellia seed cake extract has a certain inhibitory activity of 5α-reductase type Ⅱ. Through molecular docking simulation, it is speculated that the binding energy of kaempferol 3-O-[2-O-β-D-xylose-6-O-α-L-rhamnose]-β-D-glucoside to 5α-reductase is close to that of finasteride, which is considered as the main active molecule of 5α-reductase inhibitor in the total flavonoid of camellia seed cake. Figures and Tables | References | Related Articles | Metrics

Reviews

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Research progress of nickel-based catalysts mediated by （quasi） ionic liquids Zhang Chenyun, Zhang Guoxin, Shen Jinghong, Jin Jianjiao, Xu Mingxing, Xin Bingwei 2023, 53 (3):  316-324.  doi: 10.3969/j.issn.1001-1803.2023.03.010 Abstract ( 191 )   HTML ( 9 )   PDF (1876KB) ( 340 )   Owing to their special compositions and structures, ionic liquids （ILs） and deep eutectic solvents （DESs）, which are collectively referred to as （quasi） ionic liquids, have unique physical and chemical properties, such as low melting point and vapor pressure. For decades, the use of （quasi） ionic liquids to prepare catalysts with novel structure and excellent performance has attracted wide attention in the field of water electrolysis. Nickel-based electrocatalysts, as a kind of excellent catalysts for water electrolysis, have been extensively studied in （quasi） ionic liquids. In this review, the research progress of nickel-based catalysts in （quasi） ionic liquids is reviewed according to their types. （1） IL or DES-mediated nickel and nickel-based alloy. Due to the good conductivity of （quasi） ionic liquids and the ability to change the order of metal activity, the （quasi） ionic liquids can be used as media to prepare nickel metal catalysts by electrodeposition methods or galvanic replacement reactions. Furthermore, nickel salts can be designed as a component of （quasi） ionic liquids to obtain binary or ternary alloys with other metal salts. （2） IL or DES-mediated nickel （hydro）oxide. The special liquid structures of （quasi） ionic liquids can regulate the growth process of （hydro）oxide, thereby optimizing the structures and properties of catalysts. （3） IL or DES-mediated nickel-based sulfide. （Quasi） ionic liquids can not only be used as media but also be rationally designed as S sources by designing the compositions. （4） IL or DES-mediated nickel-based phosphides. ILs as media and templates can overcome the shortcomings of conventional solvents. Novel structure and pure phase can be obtained. Meanwhile, the anion and cation of ILs can be rationally designed as phosphorus sources to obtain catalyst with excellent catalytic performance. （5） IL or DES-mediated nickel-based nitride. Conventional imidazolium-type IL cations contain nitrogen, while anions can also involve nitrogen. Therefore, the use of ILs to prepare nickel-based nitrides or nitrogen-doped nickel-based catalysts has attracted much attention. In summary, combining with the work of our research group, as described in this review, （quasi） ionic liquids can be used as media, templates, and reactants for preparation of nickel-based catalysts, by which not only excellent performance will be obtained, but also the reaction system is simplified, thus to reduce emissions and realize atomic economy. Finally, the possible future development direction of this field is proposed. Figures and Tables | References | Related Articles | Metrics

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Research status and prospect of adaptogenic plants in skin health Liu Xiaoxing, Chen Chunyu, Li Li, Yi Fan 2023, 53 (3):  325-332.  doi: 10.3969/j.issn.1001-1803.2023.03.011 Abstract ( 199 )   HTML ( 20 )   PDF (1605KB) ( 227 )   Adaptogenic plants are a class of medicinal plants that can non-specifically enhance human resistance, act in multiple ways and with multiple targets, and affect the neuro-endocrine-immune system. The species resources of adaptive plants are abundant. At present, there are relatively complete researches on the effect of its oral administration on the human body, but there are few studies providing its external useon the skin. Based on bibliometrics, patent analysis and mechanisms of efficacy of adaptogenic plants in cosmetic applications, this paper finds out that the effect of adaptogenic plants on the skin are mainly focusing on anti-aging. It is further speculated that the common mechanism may be the direct action on the skin, affecting the central HPA-like axis involved in CRH in the skin; or it can directly act on the antioxidant defense system in skin cells and inhibit cell damage caused by oxidative stress. This study can provide a theoretical basis for the application of adaptogenic plants in the field of skin health. And getting fully use of the wide variety of high-value adaptogenic plant resources is also conducive to the better development of the cosmetic industry in China. Figures and Tables | References | Related Articles | Metrics

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Research on the design of daily washing packaging box based on parent-child emotion Xu Liang, Xie Hongyuan 2023, 53 (3):  333-338.  doi: 10.3969/j.issn.1001-1803.2023.03.012 Abstract ( 141 )   HTML ( 5 )   PDF (1149KB) ( 187 )   The emotional design of the packaging of parent-child daily toiletries meets the psychological needs of the audience for the purpose of cultivating parent-child feelings. Therefore, the design of daily toiletries packaging box is studied based on parent-child feelings. The consumer psychology of daily toiletries consumers is analyzed, and the design style favored by consumers is determined. According to different styles, from the perspective of parent-child emotion, the safety principle of protecting children’s health, the aesthetic principle of stimulating children’s aesthetics, and the interactive principle of conveying parent-child emotion are taken as the design criteria. using the basic visual elements such as text, graphics, color, combined with different tactile elements and modeling structural elements, the design of parent-child daily use coefficient packaging box is completed by several methods such as interactive experience method, first-level induction and combination method. Figures and Tables | References | Related Articles | Metrics

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Research hotspots in the field of skin microecology based on bibliometrics Di Feiqian, Cheng Wenjing, Li Luyao, Zhang Jiachan, Wang Changtao, An Quan 2023, 53 (3):  339-348.  doi: 10.3969/j.issn.1001-1803.2023.03.013 Abstract ( 183 )   HTML ( 3 )   PDF (2441KB) ( 306 )   Skin microbiome is closely related to skin health. It plays an important role in maintaining skin health, resisting the invasion of foreign microorganisms and participating in skin tissue metabolism. It also provides ideas for the research and development of skin therapeutic drugs and skin care products. In recent years, the literatures on skin microecology are increasing year by year. With the help of literature measurement method and knowledge map visualization software VOSviewer, this paper made quantitative statistics and comparative analysis on the distribution trend, distribution volume of major countries, distribution institutions and research topics from 2010 to 2022. The results show that in recent years, the research in the field of skin microecology has attracted worldwide attention. Its research topics mainly include “skin diseases caused by microecological imbalance and the relationship, pathogenesis and mechanism between some skin diseases and skin microorganisms”, “skin problems caused by harmful flora”, “diversity of skin microorganisms and influencing factors of colonization”, and “genetic correlation of skin microbial community”. The majority researches focus on the imbalance of skin microecology and the diversity of skin microorganisms. Then, the differences in research fields between China and the United States are analyzed and compared. The United States researchers mainly focus on the technical means of skin microorganisms, and the Chinese mainly focus on the research of three skin diseases. Figures and Tables | References | Related Articles | Metrics

Analytical methods and testing

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Determination of 18 preservatives in infant cosmetics by gas chromatography-mass spectrometry Li Yuli, Xiao Ruina, Li Yanan, Tang Qikui, Wang Qilin, Han Xiaoping 2023, 53 (3):  349-354.  doi: 10.3969/j.issn.1001-1803.2023.03.014 Abstract ( 160 )   HTML ( 9 )   PDF (1171KB) ( 192 )   A gas chromatography-mass spectrometry （GC-MS） method was established for the determination of 18 preservatives compounds （methyl chloride isothiazolinone, etc.） in infant cosmetics. The cosmetic samples were extracted with 0.1 mg/mL ascorbic acid methanol as the solvent, separated on an Agilent DB-5 MS chromatographic column （30 m×0.25 mm×0.25 μm） by temperature programming with split injection technique, analyzed by GC-MS, and then quantified by an external standard curve. The results indicate that the effective separation and determination of 18 preservatives can be realized within 20 minutes. The method has good linear relationship with the correlation coefficients （R） greater than 0.998, the limits of detection （LOD） are in the ranges of 0.15-1.50 μg/kg, and the average recoveries at three spiked levels are in the range of 76.7%-104.9%. The method is accurate, rapid, simple, and quite suitable for the simultaneous determination of 18 preservatives in infant cosmetics. Figures and Tables | References | Related Articles | Metrics

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Determination of methyl salicylate in 6 kinds of cosmetics bases by GC Wang Yajing, Jia Boyang, Wu Guoqing, Di Zheng 2023, 53 (3):  355-360.  doi: 10.3969/j.issn.1001-1803.2023.03.015 Abstract ( 371 )   HTML ( 16 )   PDF (1219KB) ( 178 )   A method for the determination of methyl salicylate in 6 kinds of cosmetics bases by gas chromatography （GC） was established, and gas chromatography-mass spectrometry （GC-MS） was used to analyze for qualitative verification. The wax-based samples were extracted with ethyl acetate-absolute ethanol, and the other bases were extracted with anhydrous ethanol, and then were determined by GC equipped with hydrogen flame ionization detector （FID）. GC-MS adopt full SCAN and selective ion monitoring （SIM） methods to confirm the results. Linear relationship between the mass concentration of methyl salicylate and its peak area is kept in the range of 5-500 mg/L （r=0.999 9）, with the detection limit of 4 μg/g, and the quantification limit of 100 μg/g. The average recovery rate of the six bases spiked at high, medium and low concentrations are 96.9%-108.2%, and the RSDs （n=6） are all less than 2.9%. The method is used to analyze different commercially available samples. The content of methyl salicylate in one batch of the lip balm is 0.083%, which exceeds the maximum concentration recommended by Scientific Committee on Consumer Safety （SCCS）. The method is fast and accurate, suitable for the qualitative and quantitative analysis of methyl salicylate in different cosmetic bases, and provides technical support for the determination and screening of methyl salicylate in cosmetics. Figures and Tables | References | Related Articles | Metrics

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Determination of polysaccharides in Gleditsia sinensis seed by high performance gel filtration chromatography Wang Zeyun, Zhang Boli 2023, 53 (3):  361-364.  doi: 10.3969/j.issn.1001-1803.2023.03.016 Abstract ( 151 )   HTML ( 7 )   PDF (1069KB) ( 132 )   High performance gel filtration chromatography was used to determine the polysaccharide components in sapiens. The hydrogel column Ultra-hydrogel™ Linear 300 mm×7.8 mmid×2 was used as the separation column with the mobile phase of 0.1mol/L sodium nitrate, the flow rate of 0.8 mL/min, and the column temperature at 40 ℃. Test results show that the polysaccharide components 1# （Mw=2 115 637）, 2#（Mw=12 516）, 3#（Mw=418） are in the range of 0.10-5.00 g/L, the peak areas and mass concentrations appear good linear correlation with the correlation coefficient γ of 0.999 5, 0.999 2, and 0.999 0（n=5）, respectively. The detection limits are 0.056, 0.053, and 0.050 g/L, respectively, the recoveries of this method are 96.7%-102.0%, 96.0%-101.6%, and 94.0%-103.6%, respectively, and the relative standard deviations are 0.63%-1.81%, 0.81%-1.94%, and 1.01%-2.03%（n=6）, respectively. The method is simple, has good repeatability and recovery, and can be used for the determination of polysaccharide components in sapiens. Figures and Tables | References | Related Articles | Metrics