Abstract:

In recent years, brown rice has gained growing attention due to its obvious advantage of being rich in bioactive compounds. The objective of this study was to evaluate the anti-aging potential of fermented brown rice filtrate （FBR） by Saccharomyces cerevisiae for cosmetics. Results for the FBR were compared to the brown rice aqueous solution （BR） that was not fermented. In this study, the total matter content of brown rice is almost unchanged before and after fermentation, but the small molecular matter increases obviously. In a DPPH free radical scavenging test, the antioxidant activity is greater in FRG compared to RG. In a human epidermal keratinocyte （HaCaT） proliferation test, FBR with a volume fraction of 0.2% and 1% shows the high proliferative capacity, while the BR with a volume fraction of 1% is characterized by high cytotoxicity to HaCaT. Meanwhile FBR shows anti-aging activity by increasing the expression of autophagy marker LC3B and Collagen I after AGEs-treated, while decreasing intracellular ROS production. Further study shows that FBR can promote the production of hyaluronic acid （HA） and the expression of its receptor CD44 in HaCaT, and enhance the expression of collagen I in human primary fibroblasts （FB）. Taken together, these data indicates that FBR has the potential of anti-aging may be applied to cosmetics products.

Key words: fermented brown rice filtrate, anti-aging, autophagy, glycation