高效液相色谱法测定唇用化妆品中对位红含量

doi:10.3969/j.issn.1001-1803.2019.06.012

Abstract

Abstract:

A high performance liquid chromatography（HPLC）method for the rapid determination of para red in lip cosmetics was established. The sample was dispersed by trichloromethane, ultrasonically extracted with acetonitrile, filtered, separated by a Waters SunFire C₁₈ column（4.6 mm×250 mm, 5 μm）, and eluted equivalently with acetonitrile-water solution（volume ratio 85:15）as mobile phase. The elution was performed at a flow rate of 1.0 mL/min and the column temperature was set at 30 ℃. The analysis was done using photo-diode array detector. External standard approach was used for quantification. The results indicate that para red has a good linear relationship in the range of 0.1-10 mg/L and the correlation coefficient is 0.999 9. The limit of detection（LOD）is 0.20 μg/g, while the limit of quantification（LOQ）is within 0.60 μg/g. The average recoveries were in the range of 94.3%-103.8% at three spiked concentrations. The method is simple, rapid, accurate and reproducible, and is suitable for the determination of para red in lip cosmetics.

Key words: cosmetics, high performance liquid chromatography, para red

CLC Number:

TQ658

HOU Guang-yue,ZHOU Li-li,BIAN Ying-fang,XU Shi-ming,DU Ying,ZOU Hui-ling. Determination of para red in lip cosmetics by high performance liquid chromatography[J].China Surfactant Detergent & Cosmetics, 2019, 49(6): 415-418.

Figures/Tables 4

References 17

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待测物	加入量/（mg·L^-1）	日内测定浓度/（mg·L^-1）	日内RSD/%	日内RE/%	日间测定浓度/（mg·L^-1）	日间RSD/%	日间RE/%
对位红	0.10	0.103±0.007	6.57	2.67	0.103±0.007	6.61	2.50
	0.50	0.486±0.008	1.61	-2.90	0.483±0.008	1.63	-3.43
	10.00	9.915±0.051	0.52	-0.85	9.895±0.053	0.54	-1.05

类型	0.60 μg/g		2.00 μg/g		4.00 μg/g
类型	平均回收率/%	RSD/%	平均回收率/%	RSD/%	平均回收率/%	RSD/%
唇膏	95.8	7.6	99.2	4.6	99.4	5.7
唇蜜	103.8	5.2	95.5	6.8	95.3	3.9
唇彩	96.9	5.3	98.7	8.9	100.2	4.2
唇釉	94.3	5.9	96.8	3.1	98.8	7.2

Determination of para red in lip cosmetics by high performance liquid chromatography

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