化妆品中耐热大肠菌群快速检测方法的研究

doi:10.3969/j.issn.1001-1803.2019.06.011

Abstract

Abstract:

The study aimed to investigate the rapid detection method of thermotolerant coliforms in cosmetics. The 3M Petrifilm ^TM coliform count plate method and Loop-mediated isothermal amplification（LAMP）technique were used to rapidly detect thermotolerant coliform bacteria in artificially contaminated cosmetics, and compared with the traditional flat plate detection method. The results show that the 3M Petrifilm ^TM coliform test method and the traditional detection method have no statistical difference, and the operation procedure is simple. The LAMP technique has strong specificity, sensitivity of 2.03 μg/L, and is time-consuming. 3M Petrifilm ^TM coliform count plate method and LAMP technique can be used for rapid detection of thermotolerant coliforms in cosmetics.

Key words: cosmetics, coliforms, count plate, Loop-mediated isothermal amplification technique

CLC Number:

TQ658

WEN Xia,LI Cai-ling,LIU Jing-xia,YANG Xiu-jiang,ZHANG Shu-yao,XIE Xiao-bao. Research on rapid detection method of thermotolerant coliforms in cosmetics[J].China Surfactant Detergent & Cosmetics, 2019, 49(6): 410-414.

Figures/Tables 7

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References 13

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来源	菌株名称	编号
标准菌株	大肠杆菌（Escherichia coli）（ATCC No.8039）	A
	铜绿假单胞菌（Pseudomonas aeruginosa）（ATCC No.9027）	B
	金黄色葡萄球菌（Staphylococcus aureus）（ATCC No.6538）	C
从污染的化妆品中分离的菌株	洋葱伯克霍尔德菌（Burkholderia cepacia）	D
	阴沟肠杆菌（Enterobacter cloacae）	E
	肺炎克雷伯氏菌（Klebsiella pneumonia）	F
	粘质沙雷氏菌（Serratia marcescens）	G
	沃氏葡萄球菌（Staphylococcus warneri）	H
	弗氏柠檬酸杆菌（Citrobacter freundii）	I

目的基因	序列（5’~3’）	大小	产物/ bp
F3	CGTTGAATACGACACCCCG	19	229
B3	CGTCATCAACCATGTCGCA	19
FIP	CTGAGCAGCACCGGTGATCATGTT- TTCACTACGCACACGTAGACTG	46	82
BIP	GATGCCGCAGACTCGTGAGCTTTT- ATGATGTACGGAACGCCTAC	34
LF	TTTAACATAGTCGGCGTGCCC	21	125
LB	ATCCTGCTGGGTCGTCAG	18

	细菌编号	A	B	C	D	E	F	G	H	I	H₂O
爽肤水①	平板法	2.1×10⁶	0	0	0	2.5×10⁶	2.8×10⁶	1.7×10⁶	0	2.5×10⁶	0
	Petrifilm法	2.3×10⁶	0	0	0	2.7×10⁶	2.6×10⁶	1.5×10⁶	0	2.2×10⁶	0
	LAMP法	+	-	-	-	-	+	+	-	+	-
爽肤水②	平板法	2.3×10⁶	0	0	0	2.5×10⁶	1.4×10⁶	4.7×10⁶	0	2.6×10⁶	0
	Petrifilm法	4.3×10⁶	0	0	0	2.5×10⁶	1.6×10⁶	5.5×10⁶	0	4.2×10⁶	0
	LAMP法	+	-	-	-	-	+	+	-	+	-
爽肤水③	平板法	5.1×10⁶	0	0	0	4.5×10⁶	1.8×10⁶	3.7×10⁶	0	6.5×10⁶	0
	Petrifilm法	2.9×10⁶	0	0	0	3.7×10⁶	2.4×10⁶	3.5×10⁶	0	7.2×10⁶	0
	LAMP法	+	-	-	-	-	+	+	-	+	-

	细菌编号	A	B	C	D	E	F	G	H	I	H₂O
乳液①	平板法	1.8×10⁶	0	0	0	2.0×10⁶	2.1×10⁶	1.7×10⁶	0	1.3×10⁶	0
	Petrifilm法	1.5×10⁶	0	0	0	2.1×10⁶	2.2×10⁶	1.8×10⁶	0	1.5×10⁶	0
	LAMP法	+	-	-	-	-	+	+	-	+	-
乳液②	平板法	2.8×10⁵	0	0	0	6.5×10⁵	2.8×10⁵	6.7×10⁵	0	8.5×10⁵	0
	Petrifilm法	4.3×10⁵	0	0	0	5.7×10⁵	2.6×10⁵	5.4×10⁵	0	7.2×10⁵	0
	LAMP法	+	-	-	-	-	+	+	-	+	-
乳液③	平板法	6.1×10⁵	0	0	0	5.6×10⁵	2.8×10⁵	3.7×10⁵	0	6.5×10⁵	0
	Petrifilm法	7.3×10⁵	0	0	0	4.8×10⁵	2.6×10⁵	4.5×10⁵	0	4.7×10⁵	0
	LAMP法	+	-	-	-	-	+	+	-	+	-

	细菌编号	A	B	C	D	E	F	G	H	I	H₂O
面霜①	平板法	2.4×10⁶	0	0	0	2.1×10⁶	2.1×10⁶	1.3×10⁶	0	2.4×10⁶	0
	Petrifilm法	2.1×10⁶	0	0	0	1.7×10⁶	1.8×10⁶	1.6×10⁶	0	2.1×10⁶	0
	LAMP法	+	-	-	-	-	+	+	-	+	-
面霜②	平板法	1.7×10⁶	0	0	0	1.5×10⁶	2.0×10⁶	2.0×10⁶	0	2.6×10⁶	0
	Petrifilm法	1.3×10⁶	0	0	0	1.7×10⁶	1.6×10⁶	1.8×10⁶	0	2.2×10⁶	0
	LAMP法	+	-	-	-	-	+	+	-	+	-
面霜③	平板法	2.1×10⁵	0	0	0	5.5×10⁵	2.8×10⁵	6.7×10⁵	0	2.4×10⁵	0
	Petrifilm法	2.3×10⁵	0	0	0	5.7×10⁵	2.6×10⁵	7.5×10⁵	0	5.2×10⁵	0
	LAMP法	+	-	-	-	-	+	+	-	+	-

Research on rapid detection method of thermotolerant coliforms in cosmetics

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