三七发酵液多糖抗衰老活性研究

doi:10.3969/j.issn.1001-1803.2019.06.004

Abstract

Abstract:

The fermentation liquor of Sanqi（Panax notoginseng）was obtained by the fermentation of Sanqi with yellow rice wine yeast. Sanqi polysaccharides were separated from such liquor and then purified. The anti-aging effect of polysaccharides in fermentation liquor of Sanqi was evaluated both on biochemical level and cellular level. The results show that, the semi-inhibitory concentrations（IC₅₀）against DPPH free radical, hydroxyl radical and superoxide anion free radical are 1.528, 0.798 and 0.216 g/L, respectively. The polysaccharides from the fermentation liquor of Sanqi can significantly reduce both the levels of intracellular reactive oxygen and the content of MMP-1 in fibroblast culture medium, and they can also significantly increase the content of cell type I collagen. In the safe mass concentration range of fermented sanqi polysaccharides on fibroblasts, 10 senescence-related factors were detected, only col-Iα（type I collagen）is down-regulated, and all other factors are up-regulated, among which the expression of angiogenic factor Ang-1 is the highest. The above results indicate that the polysaccharides from Sanqi fermentation liquor has certain anti-aging effect.

Key words: yellow rice wine yeast, Sanqi polysaccharides, free radical, anti-aging, cosmetic

CLC Number:

TQ658

LIU Ping-ping,YU Dan,WANG Chang-tao,YANG Fan,LI Meng,ZHANG Jia-chan. Study on anti-aging activity of polysaccharides in Sanqi fermentation liquor[J].China Surfactant Detergent & Cosmetics, 2019, 49(6): 369-376.

Figures/Tables 15

Tab. 1

Tab. 2

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Tab. 3

Fig. 8

Fig. 9

Fig. 10

Fig. 11

Fig. 12

References 24

[1]	Qian Li, Jiang Qing, Weng Wenjie . Progress in studies on the effects of bioactive components of panax notoginseng on motor system[J]. Chinese Journal of Sports Medicine, 2014,33(2) : 155-158.
[2]	Hu Yanhong . Discussion on the intervention effect of total saponin of panax notoginseng on cerebral microvascular endothelial cells induced by hypoischemia and reperfusion based on the rip1-rip3-mlkl signaling pathway[D]. Beijing: University of Chinese Medicine, 2017.
[3]	Cheng Jiangxue . Research on patent information analysis and patent protection of Chinese medicine extract composition[D]. Beijing: University of Chinese Medicine, 2016.
[4]	Yu Dan. Analysis of active substances and antioxidant properties extracted by microbiological method [C]// Proceedings of the 11 ^th China cosmetics academic symposium. Beijing: China flavor and fragrance cosmetics industry association , 2016: 6.
[5]	Jiu Ying . Development and research of new food raw materials on the ground of sanqi[D]. Kunming: Kunming University of Science and Technology, 2015.
[6]	Calixto J B, Campos M M, Otuki M F , et al. Anti-inflammatory compounds of plant origin. Part II. modulation of pro-inflammatory cytokines, chemokines and adhesion molecules[J]. Planta Medica, 2004,70(2) : 93-103. doi: 10.1055/s-2004-815483
[7]	Liang W, Ge S, Yang L , et al. Ginsenosides Rb1 and Rg1 promote proliferation and expression of neurotrophic factors in primary Schwann cell cultures[J]. Brain Research, 2010,1357(1) : 19-25. doi: 10.1016/j.brainres.2010.07.091
[8]	Ohtani K, Mizutani K, Hatono S , et al. Sanchinan-A, a reticuloendothelial system activating arabinogalactan from sanchi-ginseng (roots of Panax notoginseng)[J]. Planta Medica, 1987,53(2) : 166-169. doi: 10.1055/s-2006-962664
[9]	Gao H, Wang F, Lien E J , et al. Immunostimulating polysaccharides from Panax notoginseng[J]. Pharmaceutical Research, 1996,13(8) : 1196-1200. doi: 10.1023/A:1016060119425
[10]	Li Yuan, Li Qiang, Jiang Ze . Research progress of flavonoids in sanqi[J]. Pharmaceutical Research, 2012,31(2) : 109-111.
[11]	Birt D F, Hendrich S, Wang W . Dietary agents in cancer prevention: flavonoids and isoflavonoids[J]. Pharmacology & Therapeutics, 2001,90(2) : 157-177.
[12]	Androutsopoulos V P, Papakyriakou A, Vourloumis D , et al. Dietary flavonoids in cancer therapy and prevention: substrates and inhibitors of cytochrome P450 CYP1 enzymes[J]. Pharmacology & Therapeutics, 2010,126(1) : 9-20.
[13]	Lu Qi, Li Xianggao . Study on constituents of volatile oil of Panax notoginseng[J]. Chinese Journal of Pharmacy, 1987,22(9) : 528-530.
[14]	Li Liming, Ren Bin, Guo Jiewen , et al. Studies on volatile components of pseudo-ginseng in different specifications[J]. Chinese Medicine, 2013,36(6) : 934-938.
[15]	Huang Fang, Meng Yiwen . Research progress of active polysaccharides[J]. Natural Product Research and Development, 1998,1(5) : 90-97.
[16]	Chen W, Zhang Y C, Zhou M M , et al. Experimental study on effect of the polysaccharides in Pananx notoginseng on oxidation index of the rat serum under the microwave radiation[J]. Chinese Journal of Radiological Health, 2009,18(2) : 184-185.
[17]	Chen X X, Gu C H, Yang M J , et al. Study on the immunoregulation effect of Panax notoginseng polysaccharides on mice[J]. Jiangsu Journal of Preventive Medicine, 2007,18(3) : 10-12.
[18]	Jiang Wenru, Bi Liangjia, Zhao Archa . Experimental study on the artificial bone repair of bone defect by combining sanqi polysaccharide with self-solidified calcium phosphate[J]. Chinese Medicine Science and Technology, 2008,15(5) : 355-356.
[19]	Wang Jian . Study on isolation, purification and immunological activity of Panax notoginseng polysaccharides[D]. Hangzhou: Zhejiang university, 2001.
[20]	Sun haiyun, Li Jianguo, Cui Yali , et al. Improvement of extraction method of casein in cow mammary epithelial cell culture medium and its effect on cell secretion function[J]. Chinese Journal of Veterinary Medicine, 2014,34(6) : 982-985.
[21]	Yu Dan, Wang Changtao, Zhao Dan , et al. Analysis of active substances and efficacy of ginseng fermentation solution[J]. Household Chemical Industry, 2008,48(2) : 94-98.
[22]	Zhao Dan, Xu Danni, Wang Dongdong , et al. Detection of ingredients and evaluation of whitening and anti-aging efficacy of the fermentation liquor of ganoderma lucidum[J]. Chemical Industry for Daily Use, 2016,46(4) : 226-230, 242.
[23]	Ma Linxiao . Effects of Panax notoginseng polysaccharides on the gene expression of human fibroblasts[D]. Kunming: Kunming Medical University, 2015.
[24]	Qiao Liang, Lu Shuliang. Pathogenesis of diabetes mellitus combined with refractory wound angiogenesis disorder [C]// 11 ^th annual meeting of burn surgery society of Chinese medical association. Shanghai:Academic Conference of Chinese Medical Association of Burn Surgery , 2010.

试剂	V/μL
10×Fast RT Buffer	2.0
RT Enzyme Mix	1.0
FQ-RT Primer Mix	2.0
RNase-Free ddH₂O	5.0

基因	方向	引物	基因	方向	引物
GADPH	F	GAGTCAACGGATTTGGTCGT	CTGF	F	GTTTGGCCCAGACCCAACTA
GADPH	R	CAGCAGGATCTGGATCTCCC	CTGF	R	GGCTCTGCTTCTCTAGCCTG
col-Iα	F	GTGCTAAAGGTGCCAATGGT	TGF-β1	F	CCCAGCATCTGCAAAGCTC
col-Iα	R	ACCAGGTTCACCGCTGTTA	TGF-β1	R	GTCAATGTACAGCTGCCGCA
α-SMA	F	CTCTTAAGAAGGGACGCGGC	VEGF-A	F	TGAGAGACTCTGGCATGATC
α-SMA	R	CAGAATCATCGCTCTGGCCT	VEGF-A	R	CCCAGGTCAGACGGACAGAA
SDF-1	F	TTTGAGATGCTTGACGTTGG	PDGF-A	F	GGTCGCTCCTGAAGCCAG
SDF-1	R	GTGGTCGTGCTGGTCCTC	PDGF-A	R	GGAGGAGAAACAGGGAGTGC
Ang-1	F	CCGACTTCATGTTTTCCACA	bFGF	F	ACCGGATGGGGGTAGTGAGCAA
Ang-1	R	ACCGGATTTCTCTTCCCAGA	bFGF	R	GGGTCTCCCGCATACTCTGGAGA

项目	IC₅₀/（g·L^-1）
项目	DPPH自由基	羟基自由基	超氧阴离子自由基
V_C溶液	0.869	1.458	0.985
三七发酵液多糖	1.528	0.798	0.216
未发酵三七多糖	1.872	0.827	0.413

Study on anti-aging activity of polysaccharides in Sanqi fermentation liquor

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 15

References 24

Related Articles 15

Metrics

Comments

Recommended 10

[1]	Jingxuan Liu, Jianming Jin, Hua Wu. Botanical cosmetic ingredients （VII）Research and development of plant antifungal [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 259-266.
[2]	Wu Bi, Xiaohong Pan, Xiaoqin Tu, Shuai Yin, Hui Sun. Analysis of the mechanism of anti-sensitive skin effect of cosmetic raw material Stephania tetrandra based on network pharmacology [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 305-312.
[3]	Yaoyao Li. Study on the anti-aging and antioxidant effects of isosinensetin [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 313-319.
[4]	Mengran Xu, Hua Zhao. Research progress on the evaluation methods of cosmetic after-sun repair efficacy [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 329-336.
[5]	Liyuan Zhang, Linqi Yan, Qiaoyuan Cheng, Lvye Qi, Rong Wang, Liuqian Huang. Determination of 14 kinds of α-hydroxy acids and hydroxy esters in cosmetics [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 353-359.
[6]	Wei Xu, Po Zou, Changyu Li, Ming Yang, Yan Lu, Huiliang Li. Determination of 36 stimulants in cosmetics by ultra performance liquid chromatography-tandem mass spectrometry [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 360-368.
[7]	Kangfu Zhou, Yixuan Zhi, Feifei Wang, Yazhuo Shang. New emulsion system and its application in cosmetics （VI）Microemulsion [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 139-148.
[8]	Zhen Xie, Wei Huang, Jinsong Zhang, Shuhuai Chen, Linji Qu, Rong Kuang. Study on biomarkers of corneal injury in the evaluation of eye irritation of cosmetics [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 161-167.
[9]	Xiaohong Pan, Ziqi Gao, Zhen Chen, Shuai Yin, Haiping Huang, Bin Hu. Discussion on the current situation of research and management on the stability of cosmetic products in China [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 201-208.
[10]	Li Lu, Fang Fang, Youlong Feng, Ling Cao. Screening for illegal addition of sulfonamides in cosmetic products using ultra-performance liquid chromatographytriple quadrupoletandem mass spectrometry with precursor ion scanning [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 216-223.
[11]	Ren Wang, Yuanyang Wu, Jia Qiao, Linqi Yan, Cen Chen, Liyuan Zhang. Study on phenoxyethanol content in children’s cosmetics on the mark and preliminary risk assessment [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 224-230.
[12]	Yixiang Lu, Liting Wu, Jimin Jiang, Hailu Chen, Xuan Huang. Determination of tolnaftate and liranaftate in cosmetics by high performance liquid chromatography and verification by high performance liquid chromatography-tandem mass spectrometry [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 231-238.
[13]	Liyuan Zhang, Qiaoyuan Cheng, Cen Chen, Zehua Li, Liuqian Huang, Lvye Qi. Determination of 3 kinds of α-hydroxy acids and their esters in cosmetics by high performance liquid chromatography [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 102-106.
[14]	Linling Lu, Hui Lu, Chunyan Min, Yefei Qian. Determination of functional components of Glycyrrhizae, Ginseng and Scutellariae in facial masks by UHPLC-MS/MS [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 107-113.
[15]	Huiduan Long, Yixiang Lu, Jianglan Qin, Keming Zhang. Simultaneous determination of 24 coumarin compounds in cosmetics by high performance liquid chromatography and verification by liquid chromatography-tandem mass spectrometry [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 114-122.