ROS响应型汉黄芩素-透明质酸“防御-修复”协同防治皮肤光老化水凝胶敷料的构建及其性能研究

doi:10.3969/j.issn.2097-2806.2026.07.007

Abstract

Abstract:

In this study, a ROS-responsive wogonin-loaded hyaluronic acid hydrogel dressing（HA-WOG）was prepared, and its formulation and preparation process were optimized. Orthogonal tests were conducted to investigate the effects of the mass fraction of oxidized hyaluronic acid（OHA）and the dosage of crosslinker adipic acid dihydrazide（ADH）on the forming performance of hydrogels. Fourier-transform infrared spectroscopy（FTIR）and scanning electron microscopy（SEM）were employed to characterize the material structure, and its drug release behavior, drug stability and antioxidant activity were evaluated. The results show that HA-WOG is pale yellow, transparent and elastic, featuring a stable three-dimensional network structure constructed via Schiff base reaction. In vitro release experiments demonstrate its outstanding ROS-responsive drug release property. The cumulative drug release rate reaches 89.99% under a 2% H₂O₂ oxidative environment, which is markedly higher than 53.26% of the PBS control group. Hydrogel encapsulation extremely significantly improves the stability of wogonin（WOG）（P < 0.001）. The free radical scavenging capacity of HA-WOG against DPPH, ABTS⁺ and hydroxyl radicals exhibits favorable concentration- and time-dependent manner. This hydrogel possesses high swelling capacity, ROS-triggered controllable drug release and excellent antioxidant activity, which has promising application prospects in the repair of photoaged skin.

Key words: hydrogel, ROS-responsive, baicalin, antioxidant, in vitro release

CLC Number:

TQ658

Jiabao Liu, Xiaolan Chen, Wenyuan Chen, Zhanzhi Wang. Development and evaluation of a ROS-responsive baicalin-hyaluronic acid “defense-repair” synergistic hydrogel dressing for photoaging-preventing[J].China Surfactant Detergent & Cosmetics, 2026, 56(7): 893-901.

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References 18

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水平	因素
水平	A w （OHA）/%	B c （ADH）/（mol/L）	C V（ADH）/μL
1	5	0.2	200
2	6	0.3	300
3	7	0.4	400

试验号	因素			含水率/ %	降解率/ %	溶胀率/ %	综合评分
试验号	A	B	C	含水率/ %	降解率/ %	溶胀率/ %	综合评分
1	1	1	1	94.47	79.14	1 194	0.644
2	1	2	2	95.28	80.01	1 167	0.700
3	1	3	3	91.32	77.05	1 241	0.509
4	2	1	2	94.09	79.76	1 205	0.702
5	2	2	3	93.76	79.02	1 221	0.682
6	2	3	1	93.29	78.13	1 230	0.654
7	3	1	3	91.97	75.04	1 244	0.434
8	3	2	1	90.11	74.89	1 256	0.319
9	3	3	2	89.79	74.76	1 258	0.300
K₁	0.626	0.609	0.547
K₂	0.679	0.567	0.567
K₃	0.359	0.488	0.550
R	0.320	0.121	0.020

来源	偏差平方和	自由度	F比	F临界值	p值
A	0.022	2	16.5	6.94	< 0.05
B	0.005	2	3.75	6.94	> 0.05
C	0.003	2	2.25	6.94	> 0.05

试验号	含水率/%	降解率/%	溶胀率/%
1	93.25	80.57	1 208
2	92.16	79.01	1 195
3	93.14	80.12	1 209
平均值	92.85	79.90	1 204
RSD%	0.64	1.01	0.65

Development and evaluation of a ROS-responsive baicalin-hyaluronic acid “defense-repair” synergistic hydrogel dressing for photoaging-preventing

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