磷脂酰胆碱包裹的白藜芦醇增强皮肤抗氧化和抗皱能力研究

doi:10.3969/j.issn.2097-2806.2025.06.010

Abstract

Abstract:

The objective of this research was to assess the potential of phosphatidylcholine-encapsulated resveratrol as a cosmetic ingredient. The hydrogen peroxide （H₂O₂） and ultraviolet A （UVA） induced human skin fibroblasts （HSF） models of skin damage were established to compare the antioxidant and anti-wrinkle properties between phosphatidylcholine-encapsulated resveratrol and unencapsulated resveratrol. The findings reveal that encapsulating resveratrol with phosphatidylcholine not only enhances skin absorption but also significantly improves its antioxidant capabilities. In the H₂O₂-induced HSF injury model, phosphatidylcholine-encapsulated resveratrol demonstrates a superior ability to neutralize reactive oxygen species （ROS） generated by H₂O₂ compared to the resveratrol group. Further analysis indicates that this enhanced functionality is associated with increased enzymatic activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and catalase （CAT） when treated with phosphatidylcholine-encapsulated resveratrol. Additionally, in UVA-irradiated HSF cells, phosphatidylcholine-encapsulated resveratrol effectively reduces the levels of matrix metalloproteinases-1 and-3 （MMP-1 and MMP-3） and increased the contents of Collagen Ⅰ and Collagen Ⅲ （Col-1 and Col-3）, demonstrating significant anti-wrinkle effects. These findings provide critical evaluation criteria and application references for enhancing cosmetic ingredients through phosphatidylcholine encapsulation, thereby advancing skincare formulations.

Key words: phosphatidylcholine-encapsulated resveratrol, resveratrol, human skin fibroblasts, antioxidant, anti-wrinkle

CLC Number:

TQ658

Hang Tie, Qi Li, Cuicui Zhu, Minjia Yuan, Liang Xu. Phosphatidylcholine-encapsulated resveratrol augments skin’s antioxidant and anti-wrinkle capabilities[J].China Surfactant Detergent & Cosmetics, 2025, 55(6): 756-766.

Figures/Tables 8

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References 23

[1]	Roh E, Kim J E, Kwon J Y, et al. Molecular mechanisms of green tea polyphenols with protective effects against skin photoaging[J]. Critical Reviews in Food Science and Nutrition, 2017, 57 (8) : 1631-1637. doi: 10.1080/10408398.2014.1003365 pmid: 26114360
[2]	Adamczyk-Grochala J, Wnuk M, Duda M, et al. Treatment with modified extracts of the microalga planktochlorella nurekis attenuates the development of stress-induced senescence in human skin cells[J]. Nutrients, 2020, 12 (4) : 1005.
[3]	Lee E S, Lee E Y, Yoon J, et al. Sarmentosamide, an anti-aging compound from a marine-derived streptomyces sp. Apmarine042[J]. Marine Drugs, 2020, 18 (9) : 463.
[4]	Afaq F, Mukhtar H. Botanical antioxidants in the prevention of photocarcinogenesis and photoaging[J]. Experimental Dermatology, 2006, 15 (9) : 678-684. doi: 10.1111/j.1600-0625.2006.00466.x pmid: 16881964
[5]	Luczaj W, Domingues M, Domingues P, et al. Changes in lipid profile of keratinocytes from rat skin exposed to chronic UVA or UVB radiation and topical application of cannabidiol[J]. Antioxidants, 2020, 9 (12) : 1178.
[6]	Liu Tao, Zhu Shan, Yang Yi, et al. Oroxylin a ameliorates ultraviolet radiation-induced premature skin aging by regulating oxidative stress via the sirt1 pathway[J]. Biomedicine & Pharmacotherapy, 2024, 171: 116110.
[7]	Zeng Yuhua, Zhou Linyun, Chen Qianzhao, et al. Resveratrol inactivates pi3k/akt signaling through upregulating bmp7 in human colon cancer cells[J]. Oncology Reports, 2017, 38 (1) : 456-464. doi: 10.3892/or.2017.5662 pmid: 28534975
[8]	Rauf A, Imran M, Butt M S, et al. Resveratrol as an anti-cancer agent: a review[J]. Critical Reviews in Food Science and Nutrition, 2018, 58 (9) : 1428-1447. doi: 10.1080/10408398.2016.1263597 pmid: 28001084
[9]	Boo Y C. Human skin lightening efficacy of resveratrol and its analogs: from in vitro studies to cosmetic applications[J]. Antioxidants, 2019, 8 (9) : 332.
[10]	Ferreira S, Domingues F. The antimicrobial action of resveratrol against listeria monocytogenes in food-based models and its antibiofilm properties[J]. Journal of the Science of Food and Agriculture, 2016, 96 (13) : 4531-4535. doi: 10.1002/jsfa.7669 pmid: 26867522
[11]	Igielska-Kalwat J, Firlej M, Lewandowska A, et al. In vivo studies of resveratrol contained in cosmetic emulsions[J]. Acta Biochimica Polonica, 2019, 66 (3) : 371-374. doi: 10.18388/abp.2019_2838 pmid: 31518087
[12]	Baek Y, Jeong E W, Lee H G. Encapsulation of resveratrol within size-controlled nanoliposomes: impact on solubility, stability, cellular permeability, and oral bioavailability[J]. Colloids and Surfaces B: Biointerfaces, 2023, 224: 113205.
[13]	Rahimpour Y, Hamishehkar H. Liposomes in cosmeceutics[J]. Expert Opinion on Drug Delivery, 2012, 9 (4) : 443-455.
[14]	Tavano L, Muzzalupo R, Picci N, et al. Co-encapsulation of lipophilic antioxidants into niosomal carriers: percutaneous permeation studies for cosmeceutical applications[J]. Colloids and Surfaces B: Biointerfaces, 2014, 114: 144-149.
[15]	Mozafari M R, Johnson C, Hatziantoniou S, et al. Nanoliposomes and their applications in food nanotechnology[J]. Journal of Liposome Research, 2008, 18 (4) : 309-327. doi: 10.1080/08982100802465941 pmid: 18951288
[16]	Shin S, Wang Liuxiang, Zheng Xinqiang, et al. Protective effect of (-)-epigallocatechin gallate against photo-damage induced by ultraviolet a in human skin fibroblasts[J]. Tropical Journal of Pharmaceutical Research, 2014, 13 (7) : 1079-1084.
[17]	Svobodova A R, Galandakova A, Sianska J, et al. Acute exposure to solar simulated ultraviolet radiation affects oxidative stress-related biomarkers in skin, liver and blood of hairless mice[J]. Biological & Pharmaceutical Bulletin, 2011, 34 (4) : 471-479.
[18]	Jadoon S, Karim S, Bin A M, et al. Anti-aging potential of phytoextract loaded-pharmaceutical creams for human skin cell longetivity[J]. Oxidative Medicine and Cellular Longevity, 2015, 2015: 709628.
[19]	Dattilo B M, Fritz G, Leclerc E, et al. The extracellular region of the receptor for advanced glycation end products is composed of two independent structural units[J]. Biochemistry, 2007, 46 (23) : 6957-6970. doi: 10.1021/bi7003735 pmid: 17508727
[20]	Fisher G J, Datta S C, Talwar H S, et al. Molecular basis of sun-induced premature skin ageing and retinoid antagonism[J]. Nature, 1996, 379 (6563) : 335-339.
[21]	Tomic N, Stevanovic M M, Filipovic N, et al. Resveratrol/selenium nanocomposite with antioxidative and antibacterial properties[J]. Nanomaterials, 2024, 14 (4) : 368.
[22]	Perazzo A, Gallier S, Liuzzi R, et al. Quantitative methods to detect phospholipids at the oil-water interface[J]. Advances in Colloid and Interface Science, 2021, 290: 102392.
[23]	Ishihara K, Mu M, Konno T. Water-soluble and amphiphilic phospholipid copolymers having 2-methacryloyloxyethyl phosphorylcholine units for the solubilization of bioactive compounds[J]. Journal of Biomaterials Science-Polymer Edition, 2018, 29 (7-9) : 844-862.

Phosphatidylcholine-encapsulated resveratrol augments skin’s antioxidant and anti-wrinkle capabilities

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