诃子水提物体内外抗菌作用及止痒效果研究

doi:10.3969/j.issn.1001-1803.2021.02.008

Abstract

Abstract:

Investigated the effects of Terminalia chebula Retz. （TRW） with water extraction on Malassezia restricta （M. restricta） in vivo and in vitro as well as its antipruritic effects on guinea pig skin pruritus model. The minimal inhibitory concentration （MIC） in vitro of TRW against M. restricta was detected by the test tube dilution method. The results show that the MIC values against M. restricta is 7.20 mg/mL. Experimental cutaneous M. restricta infection was achieved in guinea pigs by daily inoculum for 7 consecutive days, which exproled the antifungal activity of TRW in vivo. The results show that compared with the model group, the high and medium dose of TRW display a reduction of skin lesions score in the guinea pig models （P<0.05） after 7 days of administration, the negative conversion rates of low, middle and high doses of TRW are 37.5%, 50% and 62.5%, respectively, which reduce the damage of pathological tissues in guinea pigs. Histamine phosphate inducing skin pruritus in guinea pigs was adopted to observe the effect on pruritus in experimental animals. The results show that TRW obviously inhibites skin pruritus induced by histamine in guinea pigs （P<0.05）.

Key words: Terminalia chebula Retz., Malassezia restricta, fungal infection model, relieve itching

CLC Number:

TQ658

WU Xiao-shi,PAN Jing-ling,DENG Rong-rong,MO Zi-tong,TANG Chun-ping,SHEN Zhi-bing. Study of antifungal effect in vivo and in vitro and antipruritic effects of water extract of Terminalia chebula Retz.[J].China Surfactant Detergent & Cosmetics, 2021, 51(2): 127-131.

Figures/Tables 6

Tab. 1

Tab. 2

Tab. 3

Tab. 4

Fig. 1

Tab. 5

References 20

[1]	Piérard-Franchimont C, Xhauflaire-Uhoda E, Piérard G E. Revisiting dandruff[J]. Int. J. Cosmet. Sci., 2006,28(5) : 311-318. doi: 10.1111/j.1467-2494.2006.00326.x pmid: 18489295
[2]	Kerr K, Darcy T, Henry J, et al. Epidermal changes associated with symptomatic resolution of dandruff: biomarkers of scalp health[J]. International Journal of Dermatology, 2011,50(1) : 102-113. doi: 10.1111/j.1365-4632.2010.04629.x pmid: 21182510
[3]	Lee Y W, Byun H J, Kim B J, et al. Distribution of malassezia species on the scalp in korean seborrheic dermatitis patients[J]. Annals of Dermatology, 2011,23(2) : 156-161. pmid: 21747613
[4]	Zhang Qing, Zhang Yi, Wu Jiahua, et al. Application of common active ingredients in scalp care products[J]. China Surfactant Detergent & Cosmetics, 2019,42(11) : 43-48.
[5]	Lamore S D, Wondrak C G T. The topical antimicrobial zinc pyrithione is a heat shock response inducer that causes DNA damage and PARP-dependent energy crisis in human skin cells[J]. Cell Stress and Chaperones, 2010,15(3) : 309-322. pmid: 19809895
[6]	State Pharmacopoeia Committee. Pharmacopoeia of the People’s Republic of China: Volume 1, 2005 Edition[J]. Beijing: Chemical Industry Press, 2005: 129.
[7]	Yang Junrong, Sun Fangyun, Li Zhihong, et al. Chemical constituents from Terminalia chebula Retz[J]. Natural Product Research and Development, 2008 (3) : 450-451.
[8]	Hazra B, Sarkar R, Mandal B N. Comparative study of the antioxidant and reactive oxygen species scavenging properties in the extracts of the fruits of Terminalia chebula, Terminalia belerica and Emblica officinalis[J]. BMC Complementary & Alternative Medicine, 2010,10(20) : 1-15.
[9]	Kumar N, Gangappa D. Chebulagic acid from Terminalia chebula causes G1 arrest, inhibits NFκB and induces apoptosis in retinoblastoma cells[J]. BMC Complementary and Alternative Medicine, 2014,14(1) .
[10]	Zhao Xiaoxia, Guo Zhifeng. Inhibitory effect of low temperature extract of Terminalia chebula on glioma C6 cells[J]. Shandong Medical Journal, 2015,55(14) : 32-34.
[11]	Yang Chang, Li Fei, Hou Yuefei, et al. Comparision of contents of aconitum alkaloids between the decoctions of aconitum processed with myrobalan and co-decoctions of aconitum and myrobalan-principal of aconitum processed with myrobalan Ⅱ[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2013,19(4) : 130-132.
[12]	Xiang Li, Zhou Tiejun, Ye Yingchun, et al. Effects of tannins extracted from Terminalia chebula Retz on Candida albicans and its biofilm[J]. Chongqing Medicine, 2013,42(2) : 134-137.
[13]	Sajal S, Prince S, Neena C, et al. Ellagic Acid derivatives from terminalia chebula retz. downregulate the expression of quorum sensing genes to attenuate pseudomonas aeruginosa PAO1 virulence[J]. Plos One, 2013,8(1) : 53441.
[14]	Ma Lijie, Ma Yuan, Zhang Suyu, et al. The effect of khoxote extract on electrophysiological characteristics of isolated guinea pig atrium[J]. Journal of Medicine & Pharmacy of Chinese Minorities, 2006,12(5) : 55-56.
[15]	Liu Y, Bao L, Xuan L, et al. Chebulagic acid inhibits the LPS-induced expression of TNF-α and IL-1β in endothelial cells by suppressing MAPK activation[J]. Experimental & Therapeutic Medicine, 2015,10(1) : 263-268. doi: 10.3892/etm.2015.2447 pmid: 26170946
[16]	Zhao Lu, Miao Cuiping, Yang Xiujuan, et al. Research progress in Terminalia chebula and its predictive analysis on Q-marker[J]. Chinese Traditional and Herbal Drugs, 2020,51(10) : 2732-2744.
[17]	Jing Jianlin, Zhao Ping, Li Fuwei. The effect of pH on the MIC of Terminilia chebula Retz. to bacteria[J]. Nei Mongol Journal of Traditional Chinese Medicine, 2008,8(8) : 52-55.
[18]	Pang Jinglin, Liang Yuting, Liu Xiaoyun, et al. Studies on screening of traditional chinese medicines with resistance to Malassezia restricta and its active ingredient[J]. Chin. J. Derm. Venereol., 2020,34(7) : 759-764.
[19]	Kim Y R, Kim J H, Shin H J, et al. Clinical Evaluation of a new-formula shampoo for scalp seborrheic dermatitis containing extract of rosa centifolia petals and epigallocatechin gallate: A randomized, double-blind, controlled study[J]. Annals of Dermatology, 2014,26(6) : 733-738. pmid: 25473226
[20]	Li Yang. Clinical evaluation of a plant-formula shampoo for scalp seborrheic dermatitis[D]. Changchun: Jilin University, 2017.

菌种	ρ （受试药）/（mg·mL^-1）		ρ （对照药）/（μg·mL^-1）
菌种	TRW	TRA	ZPT
M. restricta	7.20±2.30	33.54±5.26	1.03±0.09

组别	涂菌后评分/分	真菌学培养阳性率/%
模型组	8.00±0	100
ZPT组	8.57±0.53	100
基质组	7.57±0.53	100
TRW低剂量组	7.50±1.31	100
TRW中剂量组	7.13±0.83	100
TRW高剂量组	8.63±0.74	100

组别	皮损评分/分
组别	用药第1 d	用药第4 d	用药第7 d
模型组	8.00±0	6.86±0.69	4.83±0.75
ZPT组	8.57±0.53	5.00±0.58	1.20±1.10
基质组	7.57±0.53	5.00±0.58	4.29±0.49
TRW低剂量组	7.50±1.31	5.00±0.93	3.71±0.95^*
TRW中剂量组	7.13±0.83	4.38±0.74	2.38±1.60^*
TRW高剂量组	8.63±0.74	4.00±0.76	2.00±1.69^*

组别	真菌学转阴率/%			组别	真菌学转阴率/%
组别	给药第1 d	给药第4 d	给药第7 d	组别	给药第1 d	给药第4 d	给药第7 d
模型组	0	0	25	TRW低剂量组	0	25	37.5
ZPT组	0	50	75	TRW中剂量组	0	25	50
基质组	0	0	25	TRW高剂量组	0	50	62.5

组别	致痒阈/μg
模型组	30.63± 23.21
正常组	28.12± 20.34
ZPT组	56.25± 26.02^*
TRW低剂量组	233.75±183.14^{* +}
TRW中剂量组	293.13±197.30^{* +}
TRW高剂量组	292.50±249.71^*+

Study of antifungal effect in vivo and in vitro and antipruritic effects of water extract of Terminalia chebula Retz.

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 20

Related Articles 15

Metrics

Comments

Recommended 10

[1]	Jingxuan Liu, Jianming Jin, Hua Wu. Botanical cosmetic ingredients （VII）Research and development of plant antifungal [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 259-266.
[2]	Cuicui Hu, Daihong Zhou, Xinwan Chen, Jialing Zhong, Canquan Mao. Efficacies and mechanisms of a formula of Chinese medicinal plants （MHC-20） against Streptococcus pyogenes [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 282-289.
[3]	Wu Bi, Xiaohong Pan, Xiaoqin Tu, Shuai Yin, Hui Sun. Analysis of the mechanism of anti-sensitive skin effect of cosmetic raw material Stephania tetrandra based on network pharmacology [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 305-312.
[4]	Yaoyao Li. Study on the anti-aging and antioxidant effects of isosinensetin [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 313-319.
[5]	Wanping Zhang, Qi Peng, Dongmei Zhang, Shilian Zheng, Wen Jiang, Lihao Gu. Mechanism and research progress of chemical leukoderma induced by 4-substituted phenols [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 320-328.
[6]	Mengran Xu, Hua Zhao. Research progress on the evaluation methods of cosmetic after-sun repair efficacy [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 329-336.
[7]	Na Ta, Mengxin Feng, Jiamin Gao, Fenglan Zhang, Gangli Wang. Supervision and use analysis of sunscreen with potential endocrine disruption risk [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 337-343.
[8]	Wenrui Zhou, Jianbiao He, Qian Jiao, Zidi Wang, Qianqian Su, Yan Jia. Research progress of the correlation between sensory evaluation and instrumental analysis of cosmetics in O/W emulsion system [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 344-352.
[9]	Liyuan Zhang, Linqi Yan, Qiaoyuan Cheng, Lvye Qi, Rong Wang, Liuqian Huang. Determination of 14 kinds of α-hydroxy acids and hydroxy esters in cosmetics [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 353-359.
[10]	Wei Xu, Po Zou, Changyu Li, Ming Yang, Yan Lu, Huiliang Li. Determination of 36 stimulants in cosmetics by ultra performance liquid chromatography-tandem mass spectrometry [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 360-368.
[11]	Kangfu Zhou, Yixuan Zhi, Feifei Wang, Yazhuo Shang. New emulsion system and its application in cosmetics （VI）Microemulsion [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 139-148.
[12]	Hongling Zhang, Lin Cheng, Haiyan Wang, Feiya Luo, Huiliang Zhang, Lei Sun. Using DPRA alternative method to evaluate the skin sensitization of 3 coumarins [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 156-160.
[13]	Zhen Xie, Wei Huang, Jinsong Zhang, Shuhuai Chen, Linji Qu, Rong Kuang. Study on biomarkers of corneal injury in the evaluation of eye irritation of cosmetics [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 161-167.
[14]	Huiyi Li, Yue Zhou, Qian Wu, Lidi Du, Jiaying Xie, Jianhua Tan. Evaluation of cosmetics in melanin inhibition efficacy [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 168-174.
[15]	Xinyu Peng, Haiyan Liang, Zixian Wen, Meiting Li, Xin Li, Xiaofeng Qiu. Impact of rheology modifiers on Bag-on-valve spray products [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 181-187.