部分市售抑菌牙膏对7种常见口腔致病菌的体外抑制评价

doi:10.3969/j.issn.1001-1803.2020.12.008

Abstract

Abstract:

In order to investigate the efficacy of commercial toothpastes with bacteriostatic effect or antibacterial effect, the cylinder plate method was introduced to test the bacteriostatic effect on common oral pathogenic bacteria. Lactobacillus acidophilus （LA）, Enterococcus faecalis （EF）, Staphylococcus aureus （SA）, Porphyromonas gingivalis （PG）, S. mutans （SM）,Streptococcus mutans clarke （SMC）, and Pseudomonas aeruginosa （PA） were mainly selected. The results show that 16 kinds of toothpastes tested have different degrees of inhibition zones against 7 common oral pathogenic bacteria. MIC and MBC experiments of the tested toothpastes were carried out simultaneously, and the advantages of the experimental methods and the actual status of the selected commercial antibacterial toothpastes were briefly analyzed. Some suggestions are provided for the specification of the efficacy claims and nominal terms of toothpaste promotion.

Key words: antibacterial toothpaste, oral pathogenic bacteria, cylinder plate method

CLC Number:

TQ658

QIU Xi-bin,SONG Fan,XU Ren-wei,LIN Shi-han,LIN Zhao,HUANG Shu-ling,XU Ya-xuan. Evaluation of in vitro inhibition of some commercial antibacterial toothpastes against 7 common oral pathogenic bacteria[J].China Surfactant Detergent & Cosmetics, 2020, 50(12): 861-866.

Figures/Tables 4

Tab. 1

Fig. 1

Tab. 2

Determination of susceptibility（$\bar{x}$±s, n=3）"

样品编号	ρ/（g·mL^-1）	抑菌圈直径/mm
样品编号	ρ/（g·mL^-1）	LA	EF	SA	PG	SM	SMC	PA
①	1.0	18.56±0.22	10.17±0.38	25.14±1.26	14.17±0.12	18.12±0.42	18.96±0.06	19.46±0.07
①	0.5	15.68±0.28	8.52±0.09	21.14±0.11	9.21±0.22	15.68±0.15	15.91±0.12	16.52±0.46
②	1.0	24.42±1.02	19.17±0.15	18.56±0.32	13.84±0.11	13.92±0.22	19.68±0.12	17.96±0.05
②	0.5	21.19±0.34	16.59±0.42	15.72±0.24	9.01±0.06	9.02±0.02	16.94±0.19	15.19±0.04
③	1.0	25.52±0.94	18.51±0.37	18.96±0.25	19.49±0.20	14.65±0.09	18.17±0.53	18.19±0.53
③	0.5	20.98±0.24	15.68±0.04	16.71±0.41	16.52±0.67	10.62±1.02	16.87±0.72	16.12±0.04
④	1.0	17.47±0.02	14.18±0.16	13.17±0.12	—	12.17±0.42	—	14.05±0.34
④	0.5	16.09±0.50	9.51±0.07	8.56±0.15	—	9.59±0.07	—	9.14±0.13
⑤	1.0	24.19±0.27	13.68±0.52	14.17±0.18	—	13.54±0.09	18.98±0.12	19.05±0.12
⑤	0.5	20.68±0.02	9.92±0.08	10.56±1.03	—	8.66±0.28	18.17±0.14	16.47±0.32
⑥	1.0	17.24±0.52	13.52±0.09	19.21±0.25	13.04±0.24	14.62±0.02	—	14.01±0.15
⑥	0.5	15.79±0.25	9.12±0.06	16.17±0.53	9.79±0.08	10.72±0.03	—	1.05±0.52
⑦	1.0	17.62±0.28	13.79±0.54	26.54±1.32	14.11±0.14	12.51±0.71	13.04±0.12	17.97±0.03
⑦	0.5	15.89±0.32	8.27±0.02	20.56±0.03	8.49±0.07	8.55±0.16	9.02±0.34	15.21±0.09
⑧	1.0	26.02±0.09	14.17±0.20	18.92±0.33	13.11±0.32	13.64±0.07	14.27±0.11	18.64±0.39
⑧	0.5	21.14±0.57	10.56±0.68	16.53±0.41	9.16±0.33	9.62±0.83	9.11±0.92	16.52±0.53
⑨	1.0	18.05±0.15	13.21±0.47	13.11±0.42	—	—	—	14.21±0.22
⑨	0.5	15.66±0.23	9.51±0.47	9.21±0.11	—	—	—	9.64±0.15
⑩	1.0	10.77±1.18	10.14±0.91	25.04±1.44	—	—	—	13.92±0.37
⑩	0.5	9.04±0.02	8.38±0.09	20.97±0.09	—	—	—	8.99±0.03
k	1.0	19.17±0.13	11.74±0.05	19.41±0.19	—	—	—	14.14±0.43
k	0.5	17.29±0.68	9.06±0.31	16.98±0.25	—	—	—	9.12±0.14
l	1.0	14.02±0.02	14.07±0.31	14.15±0.09	—	—	14.27±0.18	13.85±0.48
l	0.5	11.17±0.77	11.47±0.58	9.52±0.56	—	—	10.01±0.06	9.01±0.13
m	1.0	17.94±0.96	14.19±0.10	13.72±0.55	13.12±0.15	14.01±0.07	14.56±0.13	14.32±0.34
m	0.5	15.88±0.21	10.74±0.07	10.02±1.47	8.68±0.29	10.12±0.03	11.14±0.47	8.78±0.11
n	1.0	24.78±0.91	19.17±0.10	13.82±0.45	12.19±0.47	12.14±0.42	13.92±0.51	13.94±0.08
n	0.5	20.47±0.12	16.56±0.50	8.74±0.13	8.44±0.06	8.89±0.40	9.03±0.21	9.24±0.12
o	1.0	18.26±0.18	14.68±0.12	14.04±0.07	—	—	—	—
o	0.5	15.92±0.13	10.91±1.23	8.85±0.03	—	—	—	—
p	1.0	14.05±0.33	13.45±0.19	—	—	—	—	14.66±0.11
p	0.5	9.87±0.78	9.52±0.51	—	—	—	—	9.54±0.54
q	1.0	14.17±0.20	13.12±0.72	—	—	—	13.14±0.71	—
q	0.5	8.62±0.12	9.04±0.05	—	—	—	9.01±0.06	—
r	1.0	13.15±0.12	12.13±1.33	14.23±0.22	—	11.05±0.42	—	14.02±0.42
r	0.5	8.62±0.27	8.42±0.04	10.12±1.01	—	8.96±0.36	—	9.59±0.43
s	1.0	12.05±0.92	14.05±0.41	12.05±1.22	—	—	—	12.01±0.99
s	0.5	8.79±0.03	10.05±0.47	9.02±0.41	—	—	—	9.09±0.22
t	1.0	17.01±0.09	13.12±0.83	13.92±0.39	12.05±0.72	—	11.14±1.38	—
t	0.5	15.59±0.07	9.14±0.33	10.52±1.04	8.47±0.15	—	8.74±0.68	—
诺氟沙星	0.005	29.21±1.22	32.16±0.55	30.56±1.73	30.98±0.27	34.05±0.72	30.14±0.17	29.74±0.32
阴性对照	/	—	—	—	—	—	—	—

Tab. 2

Tab. 3

References 21

[1]	Chen Hong, Ding Xi, Zhang Xiuhua, et al. Comparison of the antibacterial potential of five Chinese herbal toothpastes against common oral bacteria[J]. Chinese Journal of Microecology, 2007 (4) : 351-353.
[2]	Li Junke. The cause of periodontal disease[J]. Journal of Hebei Medical University, 2004.
[3]	Xu Xin, He Jinzhi, Zhou Xuedong. Oral microbiota a promising predictor of human oral and systemic diseases[J]. West China Journal of Stomatology, 2015,33(6) : 555-560. pmid: 27051943
[4]	Feng Xipin, Hu Deyu, Li Gang, et al. Consensus of Chinese stomatological experts on the efficacy of toothpaste and functional toothpaste[J]. Chinese Journal of Stomatology, 2017. doi: 10.3760/cma.j.cn112144-20200908-00496 pmid: 33171567
[5]	Liu Yiran, Cao Ling, Chen Xiaochi, et al. Experimental study on bacteriostasis in vitro of Longliqi DL toothpaste containing compound enzyme on several species of oral bacterium[J]. Oral Biomedicine, 2012,3(4) : 205-207.
[6]	Xue Yi, Tan Julian, Wu Baoyi, et al. Antimicrobial effect of 15 kinds of medicated toothpaste against black pigmented Bactcroides species[J]. Chinese Journal of Conservative Dentistry, 1993 (4) : 209-238, 250.
[7]	Xu Fanxin. Impacts of FOXO1 and periopathogens on oral mucosal wound healing[D]. Dalian: Dalian University of Technology, 2014.
[8]	Dai Jing, Chen Yanbin, Chen Shan, et al. In vitro antibacterial effect of benzalkonium chloride on five common oral pathogens[J]. Chinese Journal of Tissue Engineering Research, 2018,22(18) : 2849-2854.
[9]	Li Jin. An antibacterial repair whitening toothpaste[D]. Guangzhou: South China University of Technology, 2014.
[10]	Tian Shuange. Study on three kinds of inhibition zone tests applied in evaluation of antifungal activity of oregano oil[J]. Flavour Fragrance Cosmetics, 2019 (1) : 37-41.
[11]	Pharmacopoeia of the People’s Republic of China. Chinese Pharmacopoeia Commission [S]. 2010 Edition. Beijing: Chemical Industry Press, 2010.
[12]	Florez A B, Delgado S, Mayo B. Antimicrobial suscep tibility of lactic acid bacteria isolated from acheese environment[J]. Can. J. Microbiol., 2005 (51) : 51-58.
[13]	Ministry of Health of the People’s Republic of China. Technology Standard for Disinfection[S]. 2002 Edition. Beijing: Ministry of Health of the People’s Republic of China, 2002.
[14]	Liu Ruyun. Evaluation and comparison of several common antimicrobial test methods[J]. Modern Enterprise Education, 2013 (14) : 341-342.
[15]	Fu Changchun. Analysis of factors influencing the determination of antibiotic titer by Cylinder plate method[J]. Northwest Pharmaceutical Journal, 2008 (5) : 310-311.
[16]	Zhou Fang, Xiong Haitao, Zhang Jiang, et al. The inhibitory effect of antimicrobiai peptide against four kinds of harmful microorganisms by the oxford cup method[J]. Feed Industry, 2018 (6) : 48-51.
[17]	Jiang Zuolai, Ji Haihong, Liu Ping. Effect of yunnan white medicine cream on plaque-induced gingivitis[J]. China Medical Herald, 2008 (7) : 55-56.
[18]	Li mingyu, Zhu Cailian, Liu Zheng. In vitro study on the inhibitory effect of medicated toothpaste on some oral bacterias[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2004 (1) : 24-26.
[19]	Li Jie, Li Gang, Yang Jucai. Analysis of bacteriostatic characteristics of twelve kinds of toothpastes[J]. Journal of Practical Stomatology, 1998 (3) : 14.
[20]	Liu Shuping, Jin Bo, Li Zhen. Investigate the bacteriostasis of some toothpastes and the bacteriostatic agents of toothpastes[J]. Journal of Chengdu Medical College, 2014,9(5) : 545-548.
[21]	Zhang Xinhai, Gong Shu. Study on the bacteriostatic effect of toothpaste[J]. Guangzhou Chemical Industry, 2010,38(5) : 174-175, 184.

样品编号及项目		LA	EF	SA	PG	SM	SMC	PA
①	MIC	1:8	1:16	1:512	1:32	1:128	1:64	1:128
①	MBC	1:4	1:8	1:256	1:16	1:64	1:32	1:64
②	MIC	1:256	1:32	1:64	1:32	1:64	1:128	1:64
②	MBC	1:128	1:16	1:32	1:16	1:32	1:64	1:32
③	MIC	1:256	1:64	1:32	1:64	1:32	1:64	1:64
③	MBC	1:128	1:32	1:16	1:32	1:16	1:32	1:32
④	MIC	1:64	1:16	1:32	—	1:16	—	1:32
④	MBC	1:32	1:8	1:16	—	1:8	—	1:16
⑤	MIC	1:256	1:8	1:32	—	1:16	1:64	1:128
⑤	MBC	1:128	1:4	1:16	—	1:8	1:32	1:64
⑥	MIC	1:64	1:16	1:64	1:16	1:16	—	1:32
⑥	MBC	1:32	1:8	1:32	1:8	1:8	—	1:16
⑦	MIC	1:64	1:8	1:512	1:32	1:8	1:8	1:64
⑦	MBC	1:32	1:4	1:256	1:16	1:4	1:4	1:32
⑧	MIC	1:256	1:16	1:64	1:16	1:16	1:16	1:64
⑧	MBC	1:128	1:8	1:32	1:8	1:8	1:8	1:32
⑨	MIC	1:64	1:16	1:16	—	—	—	1:16
⑨	MBC	1:32	1:8	1:8	—	—	—	1:8
⑩	MIC	1:16	1:4	1:256	—	—	—	1:16
⑩	MBC	1:8	1:2	1:128	—	—	—	1:8
k	MIC	1:64	1:8	1:128	—	—	—	1:16
k	MBC	1:32	1:4	1:64	—	—	—	1:8
l	MIC	1:8	1:16	1:16	—	—	1:16	1:16
l	MBC	1:4	1:8	1:8	—	—	1:8	1:8
m	MIC	1:64	1:16	1:16	1:16	1:16	1:32	1:16
m	MBC	1:32	1:8	1:8	1:8	1:8	1:16	1:8
n	MIC	1:256	1:128	1:8	1:16	1:8	1:16	1:16
n	MBC	1:128	1:64	1:4	1:8	1:4	1:8	1:8
o	MIC	1:64	1:16	1:8	—	—	—	—
o	MBC	1:32	1:8	1:4	—	—	—	—
p	MIC	1:8	1:8	—	—	—	—	1:16
p	MBC	1:4	1:4	—	—	—	—	1:8
q	MIC	1:8	1:8	—	—	—	1:16	—
q	MBC	1:4	1:4	—	—	—	1:8	—
r	MIC	1:8	1:8	1:16	—	1:8	—	1:16
r	MBC	1:4	1:4	1:8	—	1:4	—	1:8
s	MIC	1:16	1:8	1:8	—	—	—	1:8
s	MBC	1:8	1:4	1:4	—	—	—	1:4
t	MIC	1:64	1:8	1:16	1:8	—	1:8	—
t	MBC	1:32	1:4	1:8	1:4	—	1:4	—
阴性对照	/	—	—	—	—	—	—	—

Evaluation of in vitro inhibition of some commercial antibacterial toothpastes against 7 common oral pathogenic bacteria

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 21

Related Articles 15

Metrics

Comments

Recommended 10

牙膏编号	相关标称功效	价格/元
①	口腔抑菌牙膏	88
②	平衡菌群牙膏	59
③	酵素抑菌牙膏	66
④	杀灭抑制牙周细菌	38
⑤	草本抑菌牙膏	38
⑥	减少口中细菌	35
⑦	有效阻止菌体	60
⑧	细菌清洁率99%	46
⑨	口腔细菌防火墙	78
⑩	杀死有害细菌	85
k	深层溶菌牙膏	68
l	抑杀细菌牙膏	58
m	真正杀菌抑菌牙膏	90
n	医学级抑菌牙膏	60
o	抑制灭杀口腔细菌	75
p	牙菌隔离牙膏	53
q	清新口气	19
r	美白健齿	9
s	特效去渍	13
t	祛火抗敏	22

[1]	Jingxuan Liu, Jianming Jin, Hua Wu. Botanical cosmetic ingredients （VII）Research and development of plant antifungal [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 259-266.
[2]	Cuicui Hu, Daihong Zhou, Xinwan Chen, Jialing Zhong, Canquan Mao. Efficacies and mechanisms of a formula of Chinese medicinal plants （MHC-20） against Streptococcus pyogenes [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 282-289.
[3]	Wu Bi, Xiaohong Pan, Xiaoqin Tu, Shuai Yin, Hui Sun. Analysis of the mechanism of anti-sensitive skin effect of cosmetic raw material Stephania tetrandra based on network pharmacology [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 305-312.
[4]	Yaoyao Li. Study on the anti-aging and antioxidant effects of isosinensetin [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 313-319.
[5]	Wanping Zhang, Qi Peng, Dongmei Zhang, Shilian Zheng, Wen Jiang, Lihao Gu. Mechanism and research progress of chemical leukoderma induced by 4-substituted phenols [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 320-328.
[6]	Mengran Xu, Hua Zhao. Research progress on the evaluation methods of cosmetic after-sun repair efficacy [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 329-336.
[7]	Na Ta, Mengxin Feng, Jiamin Gao, Fenglan Zhang, Gangli Wang. Supervision and use analysis of sunscreen with potential endocrine disruption risk [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 337-343.
[8]	Wenrui Zhou, Jianbiao He, Qian Jiao, Zidi Wang, Qianqian Su, Yan Jia. Research progress of the correlation between sensory evaluation and instrumental analysis of cosmetics in O/W emulsion system [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 344-352.
[9]	Liyuan Zhang, Linqi Yan, Qiaoyuan Cheng, Lvye Qi, Rong Wang, Liuqian Huang. Determination of 14 kinds of α-hydroxy acids and hydroxy esters in cosmetics [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 353-359.
[10]	Wei Xu, Po Zou, Changyu Li, Ming Yang, Yan Lu, Huiliang Li. Determination of 36 stimulants in cosmetics by ultra performance liquid chromatography-tandem mass spectrometry [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 360-368.
[11]	Kangfu Zhou, Yixuan Zhi, Feifei Wang, Yazhuo Shang. New emulsion system and its application in cosmetics （VI）Microemulsion [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 139-148.
[12]	Hongling Zhang, Lin Cheng, Haiyan Wang, Feiya Luo, Huiliang Zhang, Lei Sun. Using DPRA alternative method to evaluate the skin sensitization of 3 coumarins [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 156-160.
[13]	Zhen Xie, Wei Huang, Jinsong Zhang, Shuhuai Chen, Linji Qu, Rong Kuang. Study on biomarkers of corneal injury in the evaluation of eye irritation of cosmetics [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 161-167.
[14]	Huiyi Li, Yue Zhou, Qian Wu, Lidi Du, Jiaying Xie, Jianhua Tan. Evaluation of cosmetics in melanin inhibition efficacy [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 168-174.
[15]	Xinyu Peng, Haiyan Liang, Zixian Wen, Meiting Li, Xin Li, Xiaofeng Qiu. Impact of rheology modifiers on Bag-on-valve spray products [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 181-187.