当归提取物对毛发生长作用的体外与体内研究

doi:10.3969/j.issn.1001-1803.2020.12.009

Abstract

Abstract:

The effects of the extract of Angelica sinensis on anti-hair loss and hair growth were studied. The efficacy of the extract of Angelica sinensis was evaluated by using different methods such as the model of 5α-reductase inhibition, animal test and human efficacy test. The results showed that this material could inhibit the activity of 5α-reductase in vitro with a dose of 0.5 g/mL. Animal test showed that it could obviously promote hair growth of C57BL/6 mice, and it could induce the hair follicle to enter from telogen into anagen. Human efficacy test showed that the hair loss symptoms could be reduced after using the scalp care essence containing the extract of Angelica sinensis for 7 weeks. In conclusion, the extract of Angelica sinensis could stimulate the hair growth of C57BL/6 mice, and it could help the patients with hair loss symptoms.

Key words: Angelica sinensis, 5α-reductase, hair growth

CLC Number:

TQ658

GAO He-yi,LEI Deng-feng,CHENG Shu-jun,CHEN Zhi-jie,LIU Ren-long. Study on the effects of Angelica sinensis on hair growth in vitro and in vivo[J].China Surfactant Detergent & Cosmetics, 2020, 50(12): 867-874.

Figures/Tables 11

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References 17

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项目	名称	w/%
A相	去离子水	至100
	丙二醇	4
	1,3-丁二醇	5
	汉生胶	0.1
B相	当归提取液（生药质量浓度1 g/mL）	50
C相	1,2-己二醇	0.5
C相	苯氧乙醇	0.5

项目	指标
菌落总数/（CFU·mL^-1）	≤1 000
霉菌、酵母菌/（CFU·mL^-1）	≤100
大肠杆菌/mL	不得检出
绿脓杆菌/mL	不得检出
金黄色葡萄球菌/mL	不得检出
汞/（mg·kg^-1）	≤1
铅/（mg·kg^-1）	≤10
砷/（mg·kg^-1）	≤2
镉/（mg·kg^-1）	≤5

组别	皮肤由粉红到灰的时间/d	灰色皮肤到长出毛的时间/d
空白对照组	18.80±1.94	25.40±1.02
25%样品组	20.20±2.48	25.40±2.33
50%样品组	21.40±4.22	25.60±4.08
100%样品组	14.60±2.87^*	20.40±2.58^*
阳性对照组	12.60±4.03^*	20.40±3.26^*

组别	当归提取物^*		何首乌提取物^[14]		生姜汁^[15]		复配原料^[16]
组别	皮肤变灰时间/d	长毛发时间/d	皮肤变灰时间/d	长毛发时间/d	皮肤变灰时间/d	长毛发时间/d	皮肤变灰时间/d	长毛发时间/d
空白对照组	17~20	24~26	14~16	19~21	14~16	19~21	14~22	20~28
阳性对照	8~16	17~23	7~10	13~17	7~10	12~14	7~12	9~15
高剂量样品	12~17	18~23	8~10	12~16	9~12	15~17	10~14	14~18
中剂量样品	17~26	21~30	12~15	13~17	9~14	16~19	10~19	15~24
低剂量样品	17~23	23~28	12~16	16~19	12~15	17~19	19~29	23~29

指标	空白对照组	阳性对照组	25%样品组	50%样品组	100%样品组
毛囊总数/个	350.00±35.36	445.83±36.80^*	245.83±36.80	308.33±30.28	437.50±46.77^*
新生毛囊数量/个	33.33±12.91	108.33±25.82^*	29.17±10.21	58.33±12.91^*	95.83±18.82^*
新生系数	0.09±0.03	0.24±0.06^*	0.12±0.03	0.19±0.05^*	0.22±0.02^*

Study on the effects of Angelica sinensis on hair growth in vitro and in vivo

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组别	受试人数	观察时间/h	斑贴试验反应人数
组别	受试人数	观察时间/h	0级	1级	2级	3级	4级
头皮护理精华液	34	0.5	34	0	0	0	0
	34	24	34	0	0	0	0
	34	48	34	0	0	0	0
空白对照	34	0.5	33	1（31#）	0	0	0
	34	24	34	0	0	0	0
	34	48	34	0	0	0	0

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