高效液相色谱法同时测定化妆品中的12种防腐剂

doi:10.13218/j.cnki.csdc.2018.03.011

Abstract

Abstract: HPLC method was established for simultaneous determination of 12 kinds of preservatives in cosmetics.Samples were extracted by methanol,then separated by Phenomenex Luna C₁₈ column (4.6 mm×250 mm,5 μm) using acetonitrile and 0.1% phosphoric acid as mobile phase with gradient elution,the detection wavelengths were 280 and 254 nm,and the column temperature was 30 ℃.The calibration curves shows good linear relationship in the range of injection volume and all the correlation coefficients are above 0.996,the average recoveries spiked at 60%,100%,140% are in the range of 95.2%-112.1% with RSD of 0.36%-2.64% (n=9).The method shows good reproducibility and durability through detection of 40 batches of samples.It can be used for simultaneous determination of 12 kinds of preservatives in cosmetics.

Key words: cosmetics, high performance liquid chromatography, preservative

CLC Number:

TQ658

HAN Xiao-ping, LI Ya-nan, ZHOU Pei. Simultaneous determination of 12 kinds of preservatives in cosmetics by high performance liquid chromatography[J].China Surfactant Detergent & Cosmetics, 2018, 48(3): 178-182.

References

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Simultaneous determination of 12 kinds of preservatives in cosmetics by high performance liquid chromatography

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