Abstract: A method for simultaneous determination of six kinds of anti-dandruff agent including hexamidine diisethionate,salicylic acid,pyrithione zinc,ketoconazole,climbazole and octopirox in hair shampoo was established by high performance liquid chromatography with an Atlantis T₃ column (4.6 mm×250 mm,5 μm).A mixture of acetonitrile-methanol aqueous solution (0.01 mol/L KH₂PO₄ and 0.5 mmol/L Na₂EDTA) was at a flow rate of 1.0 mL/min used as HPLC mobile phase for gradient elution at room temperature;while 5 μL of which was injected for analysis.Following are the results.The linearity range of hexamidine diisethionate,salicylic acid,ketoconazole and climbazole is 4-200 mg/L,pyrithione zinc is 2-100 mg/L,as well as 5-250 mg/L for octopirox.All of which have a fine linear relation with the correlation coefficients greater than 0.999.The spiked recoveries of six components vary from 96.2% to 102.0%.The relative standard deviations of intra and inter day precision are 0.17%-0.89% and 0.27%-1.30%.The limits of detection range from 5 to 25 mg/kg,also 15-75 mg/kg as the limits of quantification.

Key words: shampoo, anti-dandruff agent, high performance liquid chromatography