滨海刺芹愈伤组织提取物中4种多酚含量测定及抗氧化、抗炎活性研究

doi:10.3969/j.issn.2097-2806.2024.01.008

Abstract

Abstract:

The preparation of extracts from callus of Eryngium maritimum was conducted by hot water reflux method. A method for simultaneous determination of rosmarinic acid, cryptochlorogenic acid, hyperoside, quercetin-3-O-（6''-O-acetyl）-β-D-glucopyranoside in the callus extract of Eryngium maritimum was established based on high performance liquid chromatography. The scavenging ability of the extracts on DPPH and ABTS free radicals were determined. The screening samples were also assayed by the CCK-8 method to determine the concentration of drug administration and a model of HaCaT cell inflammation induced by Cutibacterium acnes was established to evaluate its anti-inflammatory efficacy. The results show that the extracts contain the highest amount of cryptochlorogenic acid at 5.23 mg/g, and the contents of rosmarinic acid, quercetin-3-O-（6''-O-acetyl）-β-D-glucopyranoside and hyperin were 1.30, 0.50 and 0.08 mg/g, respectively. The extracts display antioxidant and anti-inflammatory abilities. The IC₅₀ values of the extracts on DPPH and ABTS free radical scavenging ability are 0.323 9 and 0.296 5 mg/mL, respectively, and also have significant inhibitory effects on the secretion of IL-1β and IL-8 in HaCaT cells induced by Cutibacterium acnes （P<0.05）.

Key words: Eryngium maritimum L., high performance liquid chromatography, polyphenol, anti-oxidant, anti-inflammatory

CLC Number:

TQ658

Xue Xiao, Huirong Zhang, Shuowen Li, Sunhua Li, Miaomiao Guo, Li Li. Determination of four polyphenols and study on antioxidant and anti-inflammatory activities of extracts from callus of Eryngium maritimum L.[J].China Surfactant Detergent & Cosmetics, 2024, 54(1): 57-64.

Figures/Tables 13

Tab. 1

Fig. 1

Tab. 2

Tab. 3

Fig. 2

Tab. 4

Tab. 5

Tab. 6

Fig. 3

Fig. 4

Fig. 5

Fig 6

Fig 7

References 21

[1]	Kikowska M, Thiem B. In vitro systems of selected Eryngium species (E. planum, E. campestre, E. maritimum, and E. alpinum) for studying production of desired secondary metabolites (phenolic acids, flavonoids, triterpenoid saponins, and essential oil)[J]. Plant Cell and Tissue Differentiation and Secondary Metabolites: Fundamentals and Applications, 2021: 869-901.
[2]	Isermann M, Rooney P. Biological flora of the British Isles: Eryngium maritimum[J]. Journal of Ecology, 2014, 102 (3) : 789-821. doi: 10.1111/jec.2014.102.issue-3
[3]	Minasiewicz J, Borzyszkowska S, Żółkoś K, et al. Population genetic structure of the rare species Eryngium maritimum L. (Apiaceae) in the Gulf of Gdańsk: implications for conservation and management[J]. Biodiversity Research and Conservation, 2011, 24 (2) : 39-48. doi: 10.2478/v10119-011-0023-9
[4]	Kholkhal W, Ilias F, Bekhechi C, et al. Eryngium maritimum: A rich medicinal plant of polyphenols and flavonoids compounds with antioxidant, antibacterial and antifungal activities[J]. Current Research Journal of Biological Sciences, 2012, 4 (4) : 437-443.
[5]	Meot-Duros L, Le Floch G, Magné C. Radical scavenging, antioxidant and antimicrobial activities of halophytic species[J]. Journal of Ethnopharmacology, 2008, 116 (2) : 258-262. doi: 10.1016/j.jep.2007.11.024 pmid: 18164885
[6]	Traversier M, Gaslonde T, Lecso M, et al. Comparison of extraction methods for chemical composition, antibacterial, depigmenting and antioxidant activities of Eryngium maritimum[J]. International Journal of Cosmetic Science, 2020, 42 (2) : 127-135. doi: 10.1111/ics.12595 pmid: 31799709
[7]	Mejri H, Tir M, Feriani A, et al. Does Eryngium maritimum seeds extract protect against CCl4 and cisplatin induced toxicity in rats: Preliminary phytochemical screening and assessment of its in vitro and in vivo antioxidant activity and antifibrotic effect[J]. Journal of Functional Foods, 2017, 37: 363-372. doi: 10.1016/j.jff.2017.07.054
[8]	Pereira C G, Locatelli M, Innosa D, et al. Unravelling the potential of the medicinal halophyte Eryngium maritimum L.: In vitro inhibition of diabetes-related enzymes, antioxidant potential, polyphenolic profile and mineral composition[J]. South African Journal of Botany, 2019, 120 (11) : 204-212. doi: 10.1016/j.sajb.2018.06.013
[9]	Yurdakök B, Baydan E. Cytotoxic effects of Eryngium kotschyi and Eryngium maritimum on Hep2, HepG2, Vero and U138 MG cell lines[J]. Pharmaceutical Biology, 2013, 51 (12) : 1579-1585. doi: 10.3109/13880209.2013.803208 pmid: 24028780
[10]	Chen Zhijie, Wu Jiaqi, Ma Yan, et al. Advances in the biosynthesis and regulation of phenolic acids in plant food ingredients and their bioactivities[J]. Food Science, 2018, 39 (7) : 321-328. doi: 10.7506/spkx1002-6630-201807047
[11]	Muzo T, Alexandra L. Standardization of the in vitro micropropagation protocol of Eryngium (Eryngium maritimum) from axillary meristems in the LePlant company[D]. Ambato: Technical University of Ambato, 2021.
[12]	Fang Huiyong, Zhu Hong, Ding Haimai, et al. Research progress on effect factors of secondary metabolites content in callus[J]. China Journal of Chinese Materia Medical, 2014, 39 (15) : 2846-2850.
[13]	Fan Wenxia, Yang Weipeng, Liu Hanshui. Research progress on culture technologies, chemical components, and pharmacological activities of Saussurea involucrata cells[J]. China Journal of Chinese Materia Medical, 2021, 46 (14) : 3522-3528.
[14]	Efferth T. Biotechnology applications of plant callus cultures[J]. Engineering, 2019, 5 (1) : 50-59.
[15]	Qiang B, Miao J, Phillips N, et al. Recent advances in the tissue culture of American ginseng (Panax quinquefolius)[J]. Chemistry & Biodiversity, 2020, 17 (10) : e2000366.
[17]	Zhu T, Wu W, Yang S, et al. Polyphyllin I inhibits Propionibacterium acnes-induced inflammation in vitro[J]. Inflammation, 2019, 42 (1) : 35-44. doi: 10.1007/s10753-018-0870-z
[18]	Lin Y, Tang G, Jiao Y, et al. Propionibacterium acnes induces intervertebral disc degeneration by promoting iNOS/NO and COX-2/PGE2 activation via the ROS-dependent NF-κB pathway[J]. Oxidative Medicine and Cellular Longevity, 2018 (11) : 3692752.
[19]	Mo Qiuting, Li Meng, Wang Dongdong, et al. Evaluation of anti-oxidation and whitening efficacy of Salvia miltiorrhiza fermentation broth in vitro[J]. China Surfactant Detergent ＆ Cosmetics, 2021, 51 (10) : 981-989.
[20]	Cha Yufeng, Huang Jiawen, Zan Yi, et al. Evaluation of antioxidant and whitening efficacy of Prunus mume extract[J]. China Surfactant Detergent ＆ Cosmetics, 2022, 52 (2) : 172-179.
[21]	Yang Qi, Xie Chunliang, Zhou Yingjun, et al. Antioxidant activity in vitro of probiotics fermented blueberry drink[J]. Food and Fermentation Industry, 2022, 48 (9) : 112-116.

编号	种类	回归方程	相关系数（r）	线性范围/ （mg/L）
1	隐绿原酸	y=17.147x-66.697	0.999 8	9~450
2	槲皮素-3-O-（6''-O-乙酰基）-β-D-吡喃葡萄糖苷	y=27.292x+2.858 7	0.999 8	2~100
3	金丝桃苷	y=16.094x+1.085 5	0.999 3	1~ 50
4	迷迭香酸	y=24.637x+7.484 9	0.999 8	8~400

成分	峰面积/（mAU·s）					RSD/%
成分	混标-1	混标-2	混标-3	混标-4	混标-5	RSD/%
隐绿原酸	1 846.16	1 859.83	1 859.87	1 863.17	1 866.65	0.42
槲皮素-3-O-（6''-O-乙酰基）-β-D-吡喃葡萄糖苷	1 151.55	1 153.67	1 153.59	1 151.91	1 152.89	0.08
金丝桃苷	445.12	443.24	461.26	444.29	431.05	2.42
迷迭香酸	2 120.35	2 135.98	2 136.58	2 141.29	2 140.23	0.40

成分	含量/（mg/g）			平均含量/（mg/g）	RSD/%
成分	样品1	样品2	样品3	平均含量/（mg/g）	RSD/%
隐绿原酸	5.35	5.22	5.13	5.23	2.11
槲皮素-3-O-（6''-O-乙酰基）-β-D-吡喃葡萄糖苷	0.51	0.50	0.50	0.50	1.15
金丝桃苷	0.08	0.08	0.08	0.08	0
迷迭香酸	1.32	1.27	1.32	1.30	2.22

成分	峰面积/（mAU·s）				RSD/%
成分	0 h	6 h	12 h	24 h	RSD/%
隐绿原酸	1 836.16	1 829.83	1 863.17	1 866.65	1.01
槲皮素-3-O-（6''-O-乙酰基）-β-D-吡喃葡萄糖苷	1 154.55	1 153.67	1 159.91	1 152.89	0.27
金丝桃苷	445.12	433.24	444.29	441.05	1.23
迷迭香酸	2 120.35	2 135.98	2 131.29	2 140.23	0.40

成分	加标回收率/%			平均回收率/%	RSD/%
成分	样品1	样品2	样品3	平均回收率/%	RSD/%
隐绿原酸	98.21	101.54	100.35	100.03	1.69
槲皮素-3-O-（6''-O-乙酰基）-β-D-吡喃葡萄糖苷	99.25	98.74	101.27	99.75	1.34
金丝桃苷	98.26	96.88	98.59	97.91	0.93
迷迭香酸	99.57	102.86	97.24	99.89	2.83

Determination of four polyphenols and study on antioxidant and anti-inflammatory activities of extracts from callus of Eryngium maritimum L.

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 13

References 21

Related Articles 15

Metrics

Comments

Recommended 10

[1]	Liyuan Zhang, Linqi Yan, Qiaoyuan Cheng, Lvye Qi, Rong Wang, Liuqian Huang. Determination of 14 kinds of α-hydroxy acids and hydroxy esters in cosmetics [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 353-359.
[2]	Hongling Zhang, Lin Cheng, Haiyan Wang, Feiya Luo, Huiliang Zhang, Lei Sun. Using DPRA alternative method to evaluate the skin sensitization of 3 coumarins [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 156-160.
[3]	Weilan Liu, Li Li, Chunnian He, Yinmao Dong, Xiao Ling, Miaomiao Guo. Study on the inhibitory effect of total glycosides of Paeonia veitchii and its main component paeoniflorin on acne-associated inflammation [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 188-195.
[4]	Huiduan Long, Yixiang Lu, Jianglan Qin, Keming Zhang. Simultaneous determination of 24 coumarin compounds in cosmetics by high performance liquid chromatography and verification by liquid chromatography-tandem mass spectrometry [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 114-122.
[5]	Shen Yixin, Tang Lirong, Zhang Hui. Percutaneous permeation characteristics of tea polyphenols and its protective effect on human skin fibroblasts under the oxidative damage of ultraviolet A [J]. China Surfactant Detergent & Cosmetics, 2023, 53(9): 1035-1043.
[6]	Fang Tinghuan, Zheng Ting, Jiang Qing, Li Xiaoxia, Tang Lirong. Synergistic effect of silk peptides thermophilus fermentation on the skin anti-inflammatory and anti-aging activities [J]. China Surfactant Detergent & Cosmetics, 2023, 53(9): 1057-1064.
[7]	Feng Li,Zou Baisong. Simultaneous determination of 11 components in Danzhi Fangshaishuang SFP50 by HPLC [J]. China Surfactant Detergent & Cosmetics, 2023, 53(8): 968-975.
[8]	Wang Hongdan,Xu Weichang,Wang Yuan,Kong Xiangwen,Sun Lijuan. Determination of asiaticoside and madecassoside in Centella asiatica （L.） Urban by HPLC-ELSD [J]. China Surfactant Detergent & Cosmetics, 2023, 53(8): 976-980.
[9]	Han Yudi, Jin Liying, Sun Xihan, Lin Changqing, Cui Chengbi. Preparation of a lotion containing Rhodiola rosea and Panax ginseng and its antioxidant and whitening effects [J]. China Surfactant Detergent & Cosmetics, 2023, 53(6): 665-672.
[10]	Yu Haiying, Qiao Zhenyun, Li Qiyan, Hu Defu, Niu Shuijiao, Wang Faping. Determination and isomer distribution of a cosmetic raw material hydroxypropyl tetrahydropyrantriol by HPLC [J]. China Surfactant Detergent & Cosmetics, 2023, 53(6): 721-724.
[11]	Gao Ruifang, Chai Ge, Li Xiangsheng. Determination of 47 kinds of dyestuffs in oxidative hair dye by high performance liquid chromatography [J]. China Surfactant Detergent & Cosmetics, 2023, 53(6): 725-732.
[12]	Zhang Huirong, Guo Miaomiao, Chen Chen, Pan Qianyin, Zhang Ying, Li Li. The preparation process of three leguminous plant peptides and their activities [J]. China Surfactant Detergent & Cosmetics, 2023, 53(4): 423-429.
[13]	Wu Yuanyang, Zhang Liyuan, Wang Ren, He Tingting. Determination of 10 prohibited stines in 3 different matrixes of cosmetics by HPLC-MS/MS [J]. China Surfactant Detergent & Cosmetics, 2023, 53(4): 481-486.
[14]	Yani Xu, Kaiye Yang, Rongtao Zhu, Guangrong Liu, Ping Han, Zhiyun Du. Effects of compositions of bioactive peptides on skin aging in mice [J]. China Surfactant Detergent & Cosmetics, 2023, 53(12): 1421-1428.
[15]	Zheng Lixia, Gao Huiqin, Li Sa, Guo Congjia, Hao Qianying, Wu Guotai. Determination of ferulic acid and baicalin content in Gui Qin toothpaste and its pharmacodynamic study [J]. China Surfactant Detergent & Cosmetics, 2023, 53(11): 1345-1350.

时间/min	φ（A）/%	φ（B）/%	流速/（mL/min）
0	95	5	1.0
15	90	10	1.0
50	60	40	1.0
60	60	40	1.0