Abstract: A reversed-phase high performance liquid chromatography for the determination of finasteride in anti-calvous hair cosmetics was established.The sample was analyzed on a Agilent Zorbax Extend C₁₈ column (250 mm×4.6 mm,5 μm) with detecting wavelength of 220 nm at 30 ℃,and eluted by mobile phase composed of acetonitrile (CH₃CN) and KH₂PO₄ (10 mmol·L^-1) with a gradient model,at a flow rate 1.0 mL min^-1.Results showed that as the mass concentration of finasteride is in the range of 0.8-20 mg·L^-1,the relationship between the mass concentration and the corresponding peak area shows good linearity (R²=0.999 0).The recoveries of finasteride achieve 90.3%-102.0%.

Key words: sterile hair cosmetics, finasteride, high performance liquid chromatography