冷水鱼胶原蛋白的提取、特性及在美容护肤品中的应用

doi:10.3969/j.issn.2097-2806.2025.08.013

Abstract

Abstract:

Collagen is a fibrous protein and an important part of the organism. Due to its good biological properties in beauty, it has become one of the most promising functional raw materials in skin care products. Cold-water fish collagen has excellent moisturising properties, anti-aging activity, biocompatibility and safety, leading to its increasingly widespread application in beauty and skin care products. The article provides a detailed overview of the methods used to extract collagen from cold-water fish and summarizes the differences between collagen from cold-water fish and warm-water fish in terms of collagen structure, amino acid composition, thermal stability and other physicochemical properties. Cold-water fish collagen has a lower content of proline and hydroxyproline, but the serine content is significantly higher than that of the warm-water fish. Among the aquatic collagen, the thermal stability of cold-water fish collagen is poor. In addition, the article describes the factors that cause skin aging and the effects of skin aging, summarizes the mechanisms by which collagen and its derivatives delay and improve skin aging, and introduces the forms of application and product types of cold-water fish collagen in beauty and skin care products. By summarizing and analyzing the extraction methods, properties and applications of cold-water fish collagen, we aim to provide a theoretical basis for the study of collagen from cold-water fish sources and the development of raw materials for beauty and skin care products.

Key words: collagen, cold-water fish, properties, skin care products

CLC Number:

TQ658

Kuan Ou, Junjie Zhang, Xiaofeng Qiu, Rui Duan. Extraction, properties and application of cold-water fish collagen in cosmetic skin care products[J].China Surfactant Detergent & Cosmetics, 2025, 55(8): 1058-1065.

Figures/Tables 5

Tab. 1

Tab. 2

Tab. 3

Fig. 1

Fig. 2

References 58

[1]	常杰. 冷水鱼养殖与营养调控[M]. 北京: 中国农业科学技术出版社, 2015: 4-24.
[2]	范兆廷, 姜作发, 韩英. 冷水性鱼类养殖学[M]. 北京: 中国农业出版社, 2008: 4-6.
[3]	Arumugam G K S, Sharma D, Balakrishnan R M, et al. Extraction, optimization and characterization of collagen from sole fish skin[J]. Sustainable Chemistry and Pharmacy, 2018, 9: 19-26.
[4]	Rajabimashhadi Z, Gallo N, Salvatore L, et al. Collagen derived from fish industry waste: progresses and challenges[J]. Polymers, 2023, 15 (3) : 544.
[5]	Furtado Meison, Chen Liang, Chen Zehao, et al. Development of fish collagen in tissue regeneration and drug delivery[J]. Engineered Regeneration, 2022, 3 (3) : 217-231.
[6]	李秋雨, 刘红梅, 李彦, 等. 草鱼鱼鳞柠檬法脱灰工艺的优化[J]. 农产品加工, 2020 (19) : 47-50.
[7]	Vázquez J A, Hermida-Merino C, Hermida-Merino D, et al. Characterization of gelatin and hydrolysates from valorization of farmed salmon skin by-products[J]. Polymers, 2021, 13 (16) : 2828.
[8]	Derkach S R, Kuchina Y A, Baryshnikov A V, et al. Tailoring cod gelatin structure and physical properties with acid and alkaline extraction[J]. Polymers, 2019, 11 (10) : 1724.
[9]	于颖, 肖刚, 陈晚华. 鱼皮胶原蛋白的提取工艺及特性研究[J]. 上海医药, 2019, 40 (3) : 70-73.
[10]	Martins E, Fernandes R, Alves A L, et al. Skin byproducts of reinhardtius hippoglossoides (Greenland halibut) as ecosustainable source of marine collagen[J]. Applied Sciences, 2022, 12 (21) : 11282.
[11]	Zheng Y, Li Y, Ke C, et al. Comparison of structural and physicochemical characteristics of skin collagen from chum salmon (cold-water fish) and nile tilapia (warm-water fish)[J]. Foods, 2024, 13 (8) : 1213.
[12]	窦容容, 赵春青, 李桂敏, 等. 鱼皮胶原蛋白的提取、特性及其应用研究进展[J]. 食品研究与开发, 2022, 43 (9) : 212-218.
[13]	Wang L, Liang Q, Wang Z, et al. Preparation and characterisation of type Ⅰ and Ⅴ collagens from the skin of amur sturgeon (Acipenser schrenckii)[J]. Food Chemistry, 2014, 148: 410-414. doi: 10.1016/j.foodchem.2013.10.074 pmid: 24262576
[14]	Soo M H, Hyoung J L, Jun H K, et al. Characterization of acid-and pepsin-soluble collagens from flatfish skin[J]. Food Science and Biotechnology, 2010, 19 (1) : 27-33.
[15]	Nilsuwan K, Fusang K, Pripatnanont P, et al. Properties and characteristics of acid-soluble collagen from salmon skin defatted with the aid of ultrasonication[J]. Fishes, 2022, 7 (1) : 51.
[16]	Atef M, Ojagh S M, LatifI A M, et al. Biochemical and structural characterization of sturgeon fish skin collagen (Huso huso)[J]. Journal of Food Biochemistry, 2020, 44 (8) : e13256.
[17]	Tylingo R, Mania S, Panek A, et al. Isolation and characterization of acid soluble collagen from the skin of african catfish (Clarias gariepinus), salmon (Salmo salar) and baltic cod (Gadus morhua)[J]. Journal of Biotechnology & Biomaterials, 2016, 6 (2) : 1-6.
[18]	Sousa R O, Alves A L, Carvalho D N, et al. Acid and enzymatic extraction of collagen from atlantic cod (Gadus morhua) swim bladders envisaging health-related applications[J]. Journal of Biomaterials Science, Polymer Edition, 2020, 31 (1) : 20-37.
[19]	Sun J, Zhang J, Zhao D, et al. Characterization of turbot (Scophthalmus maximus) skin and the extracted acid-soluble collagen[J]. Journal of Ocean University of China, 2019, 18 (3) : 687-692.
[20]	Martins E, Reis R L, Silva T H. In vivo skin hydrating efficacy of fish collagen from Greenland halibut as a high-value active ingredient for cosmetic applications[J]. Marine Drugs, 2023, 21 (2) : 57.
[21]	马帅, 王静, 曹爱玲, 等. 不同部位鲽鱼皮酸溶性胶原蛋白的制备及理化特性分析[J]. 中国食品学报, 2018, 18 (8) : 154-161.
[22]	Sorushanova A, Delgado L M, Wu Z, et al. The collagen suprafamily: from biosynthesis to advanced biomaterial development[J]. Advanced Materials, 2019, 31 (1) : 1801651.
[23]	Shenoy M, Abdul N S, Qamar Z, et al. Collagen structure, synthesis, and its applications: a systematic review[J]. Cureus, 2022, 14 (5) : e24856.
[24]	Felician F F, Xia C, Qi W, et al. Collagen from marine biological sources and medical applications[J]. Chemistry & Biodiversity, 2018, 15 (5) : e1700557.
[25]	Gelse K, Pöschl E, Aigner T. Collagens—structure, function, and biosynthesis[J]. Advanced Drug Delivery Reviews, 2003, 55 (12) : 1531-1546. doi: 10.1016/j.addr.2003.08.002 pmid: 14623400
[26]	Yan M, Li B, Zhao X, et al. Characterization of acid-soluble collagen from the skin of walleye pollock (Theragra chalcogramma)[J]. Food Chemistry, 2008, 107 (4) : 1581-1586.
[27]	Saito M, Takenouchi Y, Kunisaki N, et al. Complete primary structure of rainbow trout type I collagen consisting of α₁(I)α₂(I) α₃(I) heterotrimers[J]. European Journal of Biochemistry, 2001, 268 (10) : 2817-2827. pmid: 11358497
[28]	王柯, 郭志文, 段蕊, 等. 水产胶原蛋白热稳定性与环境温度相关性研究进展[J]. 食品安全质量检测学报, 2024, 15 (2) : 251-257.
[29]	Akita M, Nishikawa Y, Shigenobu Y, et al. Correlation of proline, hydroxyproline and serine content, denaturation temperature and circular dichroism analysis of type Ⅰ collagen with the physiological temperature of marine teleosts[J]. Food Chemistry, 2020, 329: 126775.
[30]	Sun L, Hou H, Li B, et al. Characterization of acid-and pepsin-soluble collagen extracted from the skin of nile tilapia (Oreochromis niloticus)[J]. International Journal of Biological Macromolecules, 2017, 99: 8-14.
[31]	Anand S, Kamath S, Chuang L, et al. Biochemical and thermo-mechanical analysis of collagen from the skin of asian sea bass (Lates calcarifer) and australasian snapper (Pagrus auratus), an alternative for mammalian collagen[J]. European Food Research and Technology, 2013, 236 (5) : 873-882.
[32]	Alves A L, Marques A L, Martins E, et al. Cosmetic potential of marine fish skin collagen[J]. Cosmetics, 2017, 4 (4) : 39.
[33]	Sinthusamran S, Benjakul S, Kishimura H. Comparative study on molecular characteristics of acid soluble collagens from skin and swim bladder of seabass (Lates calcarifer)[J]. Food Chemistry, 2013, 138 (4) : 2435-2441. doi: 10.1016/j.foodchem.2012.11.136 pmid: 23497906
[34]	Xu S, Gu M, Wu K, et al. Unraveling the role of hydroxyproline in maintaining the thermal stability of the collagen triple helix structure using simulation[J]. The Journal of Physical Chemistry B, 2019, 123 (36) : 7754-7763.
[35]	Paul E J, Padmapriya A B. Thermally stable collagen from piranha and rohu with improved physical, biochemical, and morphological properties[J]. Journal of Applied Polymer Science, 2021, 138 (32) : 50796.
[36]	Le T M T, Nguyen V M, Tran T T, et al. Comparison of acid-soluble collagen characteristic from three important freshwater fish skins in Mekong Delta Region, Vietnam[J]. Journal of Food Biochemistry, 2020, 44 (9) : e13397.
[37]	Kimura S. Wide distribution of the skin type Ⅰ collagen alpha 3 chain in bony fish[J]. Comparative Biochemistry and Physiology B, Comparative Biochemistry, 1992, 102 (2) : 255-260.
[38]	Burjanadze T V. Hydroxyproline content and location in relation to collagen thermal stability[J]. Biopolymers: Original Research on Biomolecules, 1979, 18 (4) : 931-938.
[39]	刘凤鸣, 李八方, 侯虎. 等电点法纯化太平洋鳕鱼皮胶原蛋白及其结构特性研究[J]. 食品工业科技, 2019, 40 (10) : 201-206.
[40]	叶童. 三文鱼鱼皮胶原/壳聚糖自聚集行为研究[D]. 上海: 上海海洋大学, 2023.
[41]	Yan M, Li B, Zhao X, et al. Effect of concentration, pH and ionic strength on the kinetic self-assembly of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin[J]. Food Hydrocolloids, 2012, 29 (1) : 199-204.
[42]	裘丙毅, 高志红. 现代化妆品科学与技术[M]. 北京: 中国轻工业出版社, 2016: 43-79.
[43]	Al-Atif H. Collagen supplements for aging and wrinkles: a paradigm shift in the fields of dermatology and cosmetics[J]. Dermatology Practical & Conceptual, 2022, 12 (1) : e2022018.
[44]	Li Chongyang, Fu Yu, Dai Hongjie, et al. Recent progress in preventive effect of collagen peptides on photoaging skin and action mechanism[J]. Food Science and Human Wellness, 2022, 11 (2) : 218-229.
[45]	石径, 田顺风, 马淑平, 等. 胶原蛋白肽改善和延缓皮肤衰老作用功效及机制的研究进展[J]. 食品科学, 2024 (21) : 316-322.
[46]	Shin J, Kwon S, Choi J, et al. Molecular mechanisms of dermal aging and antiaging approaches[J]. International Journal of Molecular Sciences, 2019, 20 (9) : 2126.
[47]	Quan T, He T, Shao Y, et al. Elevated cysteine-rich 61 mediates aberrant collagen homeostasis in chronologically aged and photoaged human skin[J]. The American Journal of Pathology, 2006, 169 (2) : 482-490.
[48]	Quan Taihao, He Tianyuan, Voorhees John J, et al. Ultraviolet irradiation induces Smad7 via induction of transcription factor AP-1 in human skin fibroblasts[J]. The Journal of Biological Chemistry, 2005, 280 (9) : 8079-8085.
[49]	Zhao X, Zhang X, Liu D. Collagen peptides and the related synthetic peptides: a review on improving skin health[J]. Journal of Functional Foods, 2021, 86: 104-680.
[50]	武亚琪, 琚海燕, 吕永钢. 天然胶原多肽及其护肤功效的研究进展[J]. 生物医学工程学杂志, 2022, 39 (6) : 1254-1262.
[51]	Luca D C, Mikhal' Chik V E, Suprun V M, et al. Skin antiageing and systemic redox effects of supplementation with marine collagen peptides and plant-derived antioxidants: a single-blind case-control clinical study[J]. Oxidative Medicine and Cellular Longevity, 2016: 4389410.
[52]	Chen T, Hou H, Fan Y, et al. Protective effect of gelatin peptides from pacific cod skin against photoaging by inhibiting the expression of MMPs via MAPK signaling pathway[J]. Journal of Photochemistry & Photobiology B: Biology, 2016, 165: 34-41.
[53]	辛炫英, 谢庭岩, 付兴培, 等. 黄线狭鳕鱼皮胶原蛋白肽对D-半乳糖致衰老小鼠改善作用[J]. 食品研究与开发, 2023, 44 (21) : 39-45, 166.
[54]	Chen T, Hou H. Protective effect of gelatin polypeptides from pacific cod (Gadus macrocephalus ) against UV irradiation-induced damages by inhibiting inflammation and improving transforming growth factor-β/Smad signaling pathway[J]. Journal of Photochemistry & Photobiology B: Biology, 2016, 162: 633-640.
[55]	Qin X, Xu Y, Wei Y, et al. Effects of topical application of different molecular weight marine fish skin collagen oligopeptides on UVB-induced photoaging rat skin[J]. Journal of Cosmetic Dermatology, 2021, 21 (5) : 2205-2214.
[56]	付晓婷, 刘楠楠, 裴方欣. 一种含海藻酶解物和胶原蛋白的多功能乳液及其制备方法: CN201610128836.9[P]. 2018-11-06.
[57]	孔惠, 邢晓平, 孙安琪, 等. 鲑鱼皮胶原蛋白肽的保湿性研究[J]. 日用化学工业, 2017, 47 (7) : 389-393.
[58]	Avila Rodríguez M I, Rodríguez Barroso L G, Sánchez M L. Collagen: a review on its sources and potential cosmetic applications[J]. Journal of Cosmetic Dermatology, 2018, 17 (1) : 20-26. doi: 10.1111/jocd.12450 pmid: 29144022

生物分类	来源	前处理	提取方法				文献
生物分类	来源	前处理	溶剂	温度/℃	料液比（g/mL）	时间/h	文献
鲑科	大西洋鲑鱼（Salmo salar）	① 0.1 mol/L NaOH； ② H₃PO₄（pH 5~5.2）	水	45	1:2	16	[7]
	大马哈鱼（Oncorhynchus keta）	0.1 mol/L NaHCO₃	0.5 mol/L乙酸	4	1:30	24	[11]
	虹鳟（Oncorhynchus mykiss）	① 0.05 mol/L NaOH； ② 30%异丙醇	0.5 mol/L乙酸	4	1:40	48	[15]
鲟科	史氏鲟（Acipenser schrenckii）	① 3.5% NaCl； ② 0.5% Na₂CO₃	0.1%胃蛋白酶的0.01 mol/L HCl溶液	4	1:100	48	[13]
鲟科	欧洲鳇（Huso huso）	① 0.5 mol/L NaOH； ② 10%正丁醇	0.5 mol/L乙酸和胃蛋白酶	4	1:15	48	[16]
鳕科	大西洋鳕鱼（Gadus morhua）	乙醇	水	50	1:3	3	[8]
	波罗的海鳕鱼（Gadus Morhua）	① 0.1 mol/L NaOH； ② 3% H₂O₂溶液	0.5 mol/L乙酸	4	1:6	72	[17]
	大西洋鳕鱼（Gadus morhua）	① 0.15 mol/L NaOH； ② 10% 2-丙醇	0.5 mol/L乙酸和胃蛋白酶	4	1:10	72	[18]
鲆科	褐牙鲆（Paralichthys olivaceus）	0.1 mol/L NaOH	0.5 mol/L乙酸和胃蛋白酶	4	1:10	48	[14]
鲆科	大菱鲆（Scophthalmus maximus）	① 0.1 mol/L NaOH； ② 10%正丁醇	0.5 mol/L乙酸	4	1:50	48	[19]
鲽科	马舌鲽（Reinhardtius hippoglossoides）	① 10%乙醇； ② 0.1 mol/L NaOH	0.5 mol/L乙酸	4	1:20	96	[10]
	马舌鲽（Reinhardtius hippoglossoides）	① 10%乙醇； ② 0.1 mol/L NaOH	0.5 mol/L乙酸	4	1:20	96	[20]
	角木叶鲽（Pleuronichthys coconuts）	① 0.1 mol/L NaOH； ② 10%正丁醇	0.5 mol/L乙酸	4	1:30	24	[21]

种类	生存水域	热变性温度（Td） /℃
南亚野鲮（Labeo rohita）	暖水性鱼类	38.27^[35]
食人鲳（Pygocentrus nattereri）	暖水性鱼类	37.4^[35]
罗非鱼（Oreochromis mossambicus）	暖水性鱼类	30^[36]
鳕鱼（Gadus）	冷水性鱼类	16.8^[37]
鲑鱼（Salmon salar）	冷水性鱼类	20.6^[33]
鲽鱼（Pleuronectidae）	冷水性鱼类	18^[38]

影响因素	溶解性
pH	在等电点处溶解性最小
胶原蛋白浓度	浓度增加，溶解性逐渐降低
离子强度	盐浓度增加，溶解性逐渐降低
溶剂类型	溶解度随溶剂类型而改变

Extraction, properties and application of cold-water fish collagen in cosmetic skin care products

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 58

Related Articles 15

Metrics

Comments

Recommended 10

[1]	Yawen Li, Weixin Li, Fenglun Zhang, Shihong Ma, Jianxin Jiang, Liwei Zhu. Synergistic properties of the binary mixed system of natural green surfactants: Camellia oleifera saponin and sodium cocoyl glycinate [J]. China Surfactant Detergent & Cosmetics, 2025, 55(7): 831-843.
[2]	Jun Li, Han Wu. Study on the preparation and properties of graphene-polyvinyl chloride composites [J]. China Surfactant Detergent & Cosmetics, 2025, 55(5): 611-615.
[3]	Ting Sun, Xinzhi Liang, Minghao Pang, Xia Xin, Ning Feng, Hongguang Li. The molecular weight of carbon dots calculated from colligative properties and their application in estimating surface adsorption capacity [J]. China Surfactant Detergent & Cosmetics, 2025, 55(4): 422-429.
[4]	Guofang Gao, Yadan Feng, Ziwei Diao, Yongqiang Sun, Zhiyong Hu, Hailin Zhu. Effect of sodium laurate on the properties of sodium lauroyl glutamate [J]. China Surfactant Detergent & Cosmetics, 2025, 55(4): 446-452.
[5]	Xiaoyu Li,Jianping Jiang,Lifeng Xiao. Performance analysis of nanostructured lipid carriers for chemical sunscreen agents [J]. China Surfactant Detergent & Cosmetics, 2025, 55(2): 185-193.
[6]	Chujie Zhao,Liqing Wu,Qiuxing He,Zheng Yang,Lvyangguang Ye,Lihong Yuan. Current status of sea cucumber aquaculture and the research progress on skincare benefits in China [J]. China Surfactant Detergent & Cosmetics, 2025, 55(2): 225-234.
[7]	Jian Wang, Yuhui Fan, Danfeng Li, Ningwen Cheng, Ling Li, Yufeng Yu. Skin care efficacy study of recombinant humanized collagen based on in vitro level [J]. China Surfactant Detergent & Cosmetics, 2024, 54(9): 1030-1038.
[8]	Yu Lang, Tao Geng, Jingjie Zhou, Jinyuan Sun, Huibin Liang, Chunyu Wang. Synthesis and properties of narrow-distribution ethoxylate of aliphatic amine [J]. China Surfactant Detergent & Cosmetics, 2024, 54(6): 640-647.
[9]	Biluan Zhong, Jingru Liao, Zemin Huang, Ruoran Yao, Bing Du, Yu Zhang. Study on the anti-aging effect of red slate fish gelatin collagen in mice skin caused by D-galactose [J]. China Surfactant Detergent & Cosmetics, 2024, 54(5): 550-557.
[10]	Wenrui Zhou, Jianbiao He, Qian Jiao, Zidi Wang, Qianqian Su, Yan Jia. Research progress of the correlation between sensory evaluation and instrumental analysis of cosmetics in O/W emulsion system [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 344-352.
[11]	Kuankuan Gao, Suzhen Yang, Tingting Han, Yan Li, Chunying Yuan, Xinyu Mao. Research progress of royal jelly acid and its skin care efficacy [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 209-215.
[12]	Haiwei Wang, Wenbo Xiao, Xiuling Jia, Yang Xiao, Zuliang Du. Study on the growth of honeycomb-like film of basic zinc carbonate induced by bovine serum albumin LB film template [J]. China Surfactant Detergent & Cosmetics, 2024, 54(12): 1431-1436.
[13]	Chunhong Pu, Rongchang Wang, Zhongjie Hua, Jiachan Zhang, Chao Chen. Study on the repair performance of fish collagen peptides based on the UVA induced fibroblast photodamage model [J]. China Surfactant Detergent & Cosmetics, 2024, 54(11): 1375-1381.
[14]	Yueying Ma, Qi Liu, Meng Jia, Junjie Wang, Wei Wang, Hua Zhao. A study on the method of evaluating the efficacy of a firming cosmetic [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 73-79.
[15]	Gu Weiwei. Optimal design method for packaging elements of daily skin care products under visual effects [J]. China Surfactant Detergent & Cosmetics, 2023, 53(9): 1094-1100.