高效液相色谱法同时测定化妆品中24种美白成分

doi:10.3969/j.issn.2097-2806.2024.04.015

Abstract

Abstract:

The study aimed to establish a high performance liquid chromatography （HPLC） method for simultaneous determination of 24 kinds of whitening ingredient in cosmetics. The mobile phase was methanol and monopotassium phosphate solution with gradient elution, the chromatographic column was SHISEIDO MGⅡ C₁₈ column （5 μm, 4.6 mm×250 mm）, the temperature of the column was set at 25 ℃ with a flow rate of 1.0 mL/min, and the detection wavelengths were 230 nm and 250 nm. The results show that the linear correlation of 24 whitening active ingredients is good in the range of 0.3-608.0 μg/mL, and the linear relationships are all higher than 0.999. The limits of detection are 8.1-438.4 μg/g, the limits of quantitation are 60.4-1 461.4 μg/g and the average recoveries are 87.34%-103.78%, with RSD of 0.16%-4.35%. The method is simple, reliable and can be used for the determination of whitening components in cosmetics.

Key words: cosmetic, whitening components

CLC Number:

TQ658

Wei Tao, Xiang Li. Simultaneous determination of 24 whitening components in cosmetics by high performance liquid chromatography[J].China Surfactant Detergent & Cosmetics, 2024, 54(4): 484-489.

Figures/Tables 4

Tab. 1

The information of reference standards"

序号	标准品名称	标准品英文缩写	批号	纯度/%	厂家
1	维生素C磷酸酯镁	MAP	E0026719	81.0	北京曼哈格生物科技有限公司
2	抗坏血酸葡糖苷	AA-2G	E0026417	99.5	北京曼哈格生物科技有限公司
3	烟酸	NA	G137932	99.9	德国Dr.Ehrenstorfer公司
4	曲酸	KA	E0024808	99.9	北京曼哈格生物科技有限公司
5	熊果苷	ARB	FS1625224	99.9	天津阿尔塔科技有限公司
6	烟酰胺	NAM	FS1606921	99.9	天津阿尔塔科技有限公司
7	3-O-乙基抗坏血酸	EAC	E0026419	97.9	北京曼哈格生物科技有限公司
8	红景天苷	SAL	MUST-22040414	99.82	成都曼斯特生物科技有限公司
9	4-甲氧基水杨酸钾	4MSK	0724-RB-0001	97.3	成都曼斯特生物科技有限公司
10	覆盆子酮葡糖苷	INCL	08J-WDF-14-3	98	美国PANPHY公司
11	阿魏酸	FA	MUST-22011214	99.98	成都曼斯特生物科技有限公司
12	芍药苷	PAE	MUST-22042201	98.24	成都曼斯特生物科技有限公司
13	甘草苷	LIQ	MUST-22042114	99.16	成都曼斯特生物科技有限公司
14	白藜芦醇	RES	MUST-21112413	99.82	成都曼斯特生物科技有限公司
15	甘草素	LIG	MUST-22041602	99.66	成都曼斯特生物科技有限公司
16	丹皮酚	PA	MUST-22041402	99.97	成都曼斯特生物科技有限公司
17	4-正丁基间苯二酚	RUC	R30O9S73751	99	上海源叶生物科技有限公司
18	4-（α-甲基苯甲基）间苯二酚	4RE	D22A11M111826	98	上海源叶生物科技有限公司
19	甘草酸二钾盐	DG	C11684807	99	上海麦克林生化科技有限公司
20	汉黄芩素	WOG	MUST-22030917	99.77	成都曼斯特生物科技有限公司
21	二甲氧基甲苯基-4-苯基间苯二酚	DP	M28HT180825	98	上海源叶生物科技有限公司
22	4-己基间苯二酚	4HR	FS1601208	98.8	天津阿尔塔科技有限公司
23	光甘草定	GLA	MUST-22041308	99.86	成都曼斯特生物科技有限公司
24	四氢厚朴酚	TET	D25GB172581	98	上海源叶生物科技有限公司

Tab. 1

Fig. 1

Fig. 2

Tab. 2

Regression equations, correlation coefficients, linear ranges, detection limits, and recoveries of 24 kinds of whitening ingredients （n=6）"

序号	化合物	线性方程	线性范围/ （μg/mL）	r	检出限/（μg/g）	定量限/（μg/g）	回收率/%	RSD/%
1	MAP	A=16.660 4ρ-0.024 9	4.8~160.1	0.999 96	87.9	484.6	100.60	0.18
2	AA-2G	A=14.187 9ρ+0.100 7	0.5~521.3	0.999 97	21.4	69.5	103.51	0.16
3	NA	A=12.793 1ρ+0.053 7	3.1~103.7	0.999 70	173.3	577.8	97.07	0.31
4	KA	A=19.695 2ρ-0.020 1	3.1~104.1	0.999 95	141.8	516.0	101.11	0.24
5	ARB	A=2.906 9ρ+0.005 8	3.4~114.3	0.999 91	231.2	770.7	99.51	1.09
6	NAM	A=27.444 5ρ+0.030 8	0.6~608.0	0.999 80	18.4	60.4	98.73	1.04
7	EAC	A=23.977 3ρ+0.029 6	0.3~271.0	0.999 98	21.7	72.3	100.23	0.49
8	SAL	A=8.604 1ρ-0.003 7	1.4~47.5	0.999 70	49.1	140.2	102.85	0.91
9	4MSK	A=26.486 4ρ+0.075 5	3.2~106.4	0.999 96	86.3	350.4	98.48	0.20
10	INCL	A=5.500 0ρ+0.154 6	6.0~199.1	0.999 60	373.3	1 399.7	87.34	4.35
11	FA	A=43.280 2ρ-0.195 8	1.6~55.0	0.999 80	71.7	249.8	93.89	0.32
12	PAE	A=13.018 8ρ+0.005 6	3.2~107.8	0.999 94	128.9	368.1	89.28	1.03
13	LIQ	A=26.576 4ρ-0.007 6	1.9~64.2	0.999 94	67.7	251.3	98.03	1.64
14	RES	A=46.174 4ρ-0.023 8	1.7~57.6	0.999 95	28.8	254.9	95.79	0.23
15	LIG	A=42.318 3ρ-0.045 0	1.9~62.4	0.999 97	25.9	231.3	96.93	0.35
16	PA	A=41.464 2ρ+0.006 7	3.1~102.7	0.999 70	83.3	314.6	94.88	0.30
17	RUC	A=17.408 1ρ+0.007 3	3.2~108.3	0.999 80	162.5	649.8	99.02	0.56
18	4RE	A=46.177 1ρ-0.062 9	1.7~56.4	0.999 80	54.6	176.3	97.16	0.27
19	DG	A=6.128 4ρ-0.043 6	0.5~52.8	0.999 80	438.4	1 461.4	103.78	0.65
20	WOG	A=23.484 4ρ-0.111 9	1.7~57.6	0.999 97	45.9	177.3	96.65	0.47
21	DP	A=27.050 7ρ-0.023 2	3.0~101.2	0.999 60	67.5	269.7	96.65	0.67
22	4HR	A=14.595 8ρ-0.018 5	3.1~104.3	0.999 95	164.7	432.6	97.31	1.07
23	GLA	A=59.496 2ρ+0.019 5	1.3~41.7	0.999 92	18.8	166.5	96.42	0.63
24	TET	A=35.204 7ρ-0.034 9	1.0~32.3	0.999 98	8.1	102.8	101.57	0.60

Tab. 2

References 12

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Simultaneous determination of 24 whitening components in cosmetics by high performance liquid chromatography

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